Values CatLog read from this paper
Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis
PMID 10488127doi:10.1074/jbc.274.39.27815Open in Browse
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CatLog read 9 records (27 values) from this paper: pepstatin-insensitive carboxyl proteinase PCP, pepstatin-insensitive carboxyl proteinase XCP from Pseudomonas sp., Xanthomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pepstatin-insensitive carboxyl proteinase PCP | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 59.3 | 0.0074 | 8 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase PCPvariant D124A | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 39 | 0.0067 | 5.8 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP variant D124A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase PCPvariant D225A | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 65.2 | 0.0296 | 2.2 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP variant D225A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase PCPvariant D265A | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 59.5 | 0.0091 | 6.5 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP variant D265A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase PCPvariant D84A | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 0.055 | 0.149 | 0.37 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP variant D84A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase PCPvariant E217A | Pseudomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 56 | 0.0101 | 5.5 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase PCP variant E217A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase XCP | Xanthomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 68.4 | 0.0058 | 1.18 × 10⁴ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase XCP, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase XCPvariant E230A | Xanthomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 21.8 | 0.0111 | 2 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase XCP variant E230A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |
| pepstatin-insensitive carboxyl proteinase XCPvariant E235A | Xanthomonas sp. | Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu | 34.9 | 0.0188 | 1.9 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase XCP variant E235A, Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu |