CatLogenzyme kinetics catalog

EC 3.4.21.100: sedolisin

HydrolasesOpen in Browse

CatLog read 24 records for EC 3.4.21.100 from 2 papers, for 1 organism. Names in these papers: pepstatin-insensitive carboxyl proteinase, pepstatin-insensitive carboxyl proteinase PCP.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 3.4.21.100 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.100 in Browse.

Papers

PaperOrganismsRecords
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101Pseudomonas sp.18
Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesisPseudomonas sp.6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine6.20.162pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-alpha-aspartyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine5.90.09860pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine0.1 M sodium formate buffer pH 3.5; kcat = Vmax/[E]5.20.12542pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine0.1 M sodium citrate buffer pH 3.5; 6.4 nM enzyme—0.1188—pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-leucyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine40.04393pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-alanyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine11.40.1295pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-alpha-aspartyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine1—8pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-arginyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine0.90.07712pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-leucyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine3.80.03127pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine6.8—523pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-leucyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine5.50.0692pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alpha-aspartyl-L-leucine6.90.068101pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine14.50.095153pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-asparaginyl-L-glutaminyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine5.50.052110 calc.pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-isoleucyl-L-alpha-glutamyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine6.90.0032.3 × 10³pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-prolyl-L-valyl-L-seryl-L-tyrosyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine5.2—274pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-lysyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine2.20.12318pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinasePseudomonas sp.L-seryl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine5.5—100pH 3.5, 37 °CPMID 1562589Open
pepstatin-insensitive carboxyl proteinase PCPPseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu59.30.00748 × 10³pH 3.5, 37 °CPMID 10488127Open
pepstatin-insensitive carboxyl proteinase PCPvariant D124APseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu390.00675.8 × 10³pH 3.5, 37 °CPMID 10488127Open
pepstatin-insensitive carboxyl proteinase PCPvariant D225APseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu65.20.02962.2 × 10³pH 3.5, 37 °CPMID 10488127Open
pepstatin-insensitive carboxyl proteinase PCPvariant D265APseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu59.50.00916.5 × 10³pH 3.5, 37 °CPMID 10488127Open
pepstatin-insensitive carboxyl proteinase PCPvariant D84APseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu0.0550.1490.37pH 3.5, 37 °CPMID 10488127Open
pepstatin-insensitive carboxyl proteinase PCPvariant E217APseudomonas sp.Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu560.01015.5 × 10³pH 3.5, 37 °CPMID 10488127Open