Values CatLog read from this paper
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101
PMID 1562589doi:10.1016/0167-4838(92)90272-fOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (47 values) from this paper: pepstatin-insensitive carboxyl proteinase from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.2 | 0.1 | 62 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-alpha-aspartyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.9 | 0.098 | 60 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-alpha-aspartyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine0.1 M sodium formate buffer pH 3.5; kcat = Vmax/[E] | 5.2 | 0.125 | 42 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine0.1 M sodium citrate buffer pH 3.5; 6.4 nM enzyme | — | 0.1188 | — | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-leucyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 4 | 0.043 | 93 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-leucyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-alanyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 11.4 | 0.12 | 95 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-alanyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-alpha-aspartyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 1 | — | 8 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-alpha-aspartyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-arginyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 0.9 | 0.077 | 12 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-arginyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-leucyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 3.8 | 0.03 | 127 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-leucyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.8 | — | 523 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-leucyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.5 | 0.06 | 92 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-leucyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alpha-aspartyl-L-leucine | 6.9 | 0.068 | 101 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alpha-aspartyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 14.5 | 0.095 | 153 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-asparaginyl-L-glutaminyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.5 | 0.052 | 110 calc. | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-asparaginyl-L-glutaminyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-isoleucyl-L-alpha-glutamyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.9 | 0.003 | 2.3 × 10³ | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-isoleucyl-L-alpha-glutamyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-prolyl-L-valyl-L-seryl-L-tyrosyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.2 | — | 274 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-prolyl-L-valyl-L-seryl-L-tyrosyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-lysyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 2.2 | 0.123 | 18 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-lysyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| pepstatin-insensitive carboxyl proteinase | Pseudomonas sp. | L-seryl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.5 | — | 100 | pH 3.5, 37 °C | Open record: pepstatin-insensitive carboxyl proteinase, L-seryl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |