CatLogenzyme kinetics catalog

Values CatLog read from this paper

Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)

PMID 10493791doi:10.1021/bi990517rOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 44 records (103 values) from this paper: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.2.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)Escherichia coliATP—0.057117.8pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)Escherichia coliD-glutamate—0.064105pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)Escherichia coliUDP-N-acetylmuramoyl-L-alanine6.720.0051343pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317AEscherichia coliATP—2.180.01pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317AEscherichia coliD-glutamate—0.0440.48pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.0220.0570.38pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35AEscherichia coliATP—0.11437.5pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35AEscherichia coliD-glutamate—0.09544.8pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35AEscherichia coliUDP-N-acetylmuramoyl-L-alanine4.270.08450.8pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157AEscherichia coliATP—0.030.0112pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157AEscherichia coliUDP-N-acetylmuramoyl-L-alanine3.3 × 10⁻⁴0.3040.0011pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157KEscherichia coliATP—0.0850.0043pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157KEscherichia coliD-glutamate—0.0560.0065pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157KEscherichia coliUDP-N-acetylmuramoyl-L-alanine3.7 × 10⁻⁴0.2180.0017pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183AEscherichia coliATP—0.130.72pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183AEscherichia coliD-glutamate—0.8060.115pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.0930.0591.58pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301AEscherichia coliATP—0.1535pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301AEscherichia coliD-glutamate—0.0958pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.770.0419.2pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115AEscherichia coliATP—0.3280.0132pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115AEscherichia coliD-glutamate—0.1280.033pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.00430.0460.093pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliATP—0.1350.118pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliD-glutamate—0.1850.087pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.0160.120.133pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliATP—0.1290.093pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliD-glutamate—0.2160.055pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.0120.140.085pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268AEscherichia coliATP—0.1436.5pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268AEscherichia coliD-glutamate—0.26419.3pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268AEscherichia coliUDP-N-acetylmuramoyl-L-alanine5.120.012427pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271AEscherichia coliATP—1.580.18pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271AEscherichia coliD-glutamate—0.0535.7pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.30.01618.7pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302AEscherichia coliATP—21.57pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302AEscherichia coliD-glutamate—0.07442.3pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302AEscherichia coliUDP-N-acetylmuramoyl-L-alanine3.130.06548.2pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425AEscherichia coliATP—0.1832.8pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425AEscherichia coliD-glutamate—5.160.103pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.530.05310pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194FEscherichia coliATP—0.1443.7pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194FEscherichia coliD-glutamate—0.05310pH 9.4, 37 °COpen
UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194FEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.530.1114.8pH 9.4, 37 °COpen