EC 6.3.2.9: UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase
LigasesOpen in Browse
CatLog read 68 records for EC 6.3.2.9 from 3 papers, for 2 organisms. Names in these papers: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD), UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 12 records for EC 6.3.2.9 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.2.9 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | 44 |
| Evidence of a functional requirement for a carbamoylated lysine residue in MurD, MurE and MurF synthetases as established by chemical rescue experiments | Escherichia coli | 21 |
| Purification and biochemical characterization of Mur ligases from Staphylococcus aureus | Staphylococcus aureus | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | adenosine 5'-triphosphate | — | 5.4 | — | pH 8.8, 37 °C | PMID 20659527 | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), adenosine 5'-triphosphate |
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | D-glutamic acid | — | 0.13 | — | pH 8.8, 37 °C | PMID 20659527 | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), D-glutamic acid |
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | UDP-N-acetylmuramoyl-L-alanine | — | 0.041 | — | pH 8.8, 37 °C | PMID 20659527 | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | ATP | — | 0.057 | 117.8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD), ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | D-glutamate | — | 0.064 | 105 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD), D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 6.72 | 0.005 | 1343 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD), UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317A | Escherichia coli | ATP | — | 2.18 | 0.01 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D317A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317A | Escherichia coli | D-glutamate | — | 0.044 | 0.48 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D317A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D317A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.022 | 0.057 | 0.38 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D317A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35A | Escherichia coli | ATP | — | 0.114 | 37.5 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D35A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35A | Escherichia coli | D-glutamate | — | 0.095 | 44.8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D35A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant D35A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 4.27 | 0.084 | 50.8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant D35A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157A | Escherichia coli | ATP | — | 0.03 | 0.0112 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant E157A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 3.3 × 10⁻⁴ | 0.304 | 0.0011 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant E157A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157K | Escherichia coli | ATP | — | 0.085 | 0.0043 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant E157K, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157K | Escherichia coli | D-glutamate | — | 0.056 | 0.0065 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant E157K, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant E157K | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 3.7 × 10⁻⁴ | 0.218 | 0.0017 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant E157K, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183A | Escherichia coli | ATP | — | 0.13 | 0.72 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H183A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183A | Escherichia coli | D-glutamate | — | 0.806 | 0.115 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H183A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H183A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.093 | 0.059 | 1.58 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H183A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301A | Escherichia coli | ATP | — | 0.153 | 5 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H301A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301A | Escherichia coli | D-glutamate | — | 0.095 | 8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H301A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant H301A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.77 | 0.04 | 19.2 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant H301A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115A | Escherichia coli | ATP | — | 0.328 | 0.0132 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K115A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115A | Escherichia coli | D-glutamate | — | 0.128 | 0.033 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K115A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K115A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.0043 | 0.046 | 0.093 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K115A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | ATP | — | 0.135 | 0.118 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | D-glutamate | — | 0.185 | 0.087 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.016 | 0.12 | 0.133 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | ATP | — | 0.129 | 0.093 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198F, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | D-glutamate | — | 0.216 | 0.055 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198F, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.012 | 0.14 | 0.085 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant K198F, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268A | Escherichia coli | ATP | — | 0.14 | 36.5 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N268A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268A | Escherichia coli | D-glutamate | — | 0.264 | 19.3 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N268A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N268A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 5.12 | 0.012 | 427 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N268A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271A | Escherichia coli | ATP | — | 1.58 | 0.18 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N271A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271A | Escherichia coli | D-glutamate | — | 0.053 | 5.7 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N271A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant N271A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.3 | 0.016 | 18.7 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant N271A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302A | Escherichia coli | ATP | — | 2 | 1.57 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R302A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302A | Escherichia coli | D-glutamate | — | 0.074 | 42.3 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R302A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R302A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 3.13 | 0.065 | 48.2 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R302A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425A | Escherichia coli | ATP | — | 0.183 | 2.8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R425A, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425A | Escherichia coli | D-glutamate | — | 5.16 | 0.103 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R425A, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant R425A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.53 | 0.053 | 10 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant R425A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194F | Escherichia coli | ATP | — | 0.144 | 3.7 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant Y194F, ATP |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194F | Escherichia coli | D-glutamate | — | 0.053 | 10 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant Y194F, D-glutamate |
| UDP-MurNAc-L-alanine:D-glutamate ligase (MurD)variant Y194F | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine | 0.53 | 0.111 | 4.8 | pH 9.4, 37 °C | PMID 10493791 | Open record: UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) variant Y194F, UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | adenosine 5'-triphosphateno acid added | — | 0.06 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | adenosine 5'-triphosphate0.5 M formate added (stock titrated to assay pH with KOH) | — | 0.138 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | adenosine 5'-triphosphate0.5 M acetate added (stock titrated to assay pH with KOH) | — | 0.088 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | D-glutamic acidno acid added | — | 0.072 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | D-glutamic acid0.5 M formate added (stock titrated to assay pH with KOH) | — | 0.04 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | D-glutamic acid0.5 M acetate added (stock titrated to assay pH with KOH) | — | 0.132 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | UDP-N-acetylmuramoyl-L-alanineno acid added | 6.67 | 0.005 | 1.3 × 10³ calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine0.5 M formate added (stock titrated to assay pH with KOH) | 1.82 | 0.005 | 360 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine0.5 M acetate added (stock titrated to assay pH with KOH) | 1.48 | 0.005 | 300 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD), UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | adenosine 5'-triphosphateno acid added | — | 0.215 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | adenosine 5'-triphosphate0.5 M acetate added (stock titrated to assay pH with KOH) | — | 0.216 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | D-glutamic acidno acid added | — | 0.283 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | D-glutamic acid0.5 M acetate added (stock titrated to assay pH with KOH) | — | 0.188 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanineno acid added | 0.0108 | 0.165 | 0.0660 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198A | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine0.5 M acetate added (stock titrated to assay pH with KOH) | 0.282 | 0.256 | 1.10 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198A, UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | adenosine 5'-triphosphateno acid added | — | 0.119 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | adenosine 5'-triphosphate0.5 M formate added (stock titrated to assay pH with KOH) | — | 0.144 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, adenosine 5'-triphosphate |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | D-glutamic acidno acid added | — | 0.688 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | D-glutamic acid0.5 M formate added (stock titrated to assay pH with KOH) | — | 0.485 | — | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, D-glutamic acid |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | UDP-N-acetylmuramoyl-L-alanineno acid added | 0.0073 | 0.122 | 0.060 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, UDP-N-acetylmuramoyl-L-alanine |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198F | Escherichia coli | UDP-N-acetylmuramoyl-L-alanine0.5 M formate added (stock titrated to assay pH with KOH) | 0.0895 | 0.559 | 0.160 calc. | pH 9.4, 37 °C | PMID 11722566 | Open record: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) variant K198F, UDP-N-acetylmuramoyl-L-alanine |