CatLogenzyme kinetics catalog

Values CatLog read from this paper

Evidence of a functional requirement for a carbamoylated lysine residue in MurD, MurE and MurF synthetases as established by chemical rescue experiments

PMID 11722566doi:10.1046/j.0014-2956.2001.02524.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (28 values) from this paper: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.2.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliadenosine 5'-triphosphateno acid added—0.06—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliadenosine 5'-triphosphate0.5 M formate added (stock titrated to assay pH with KOH)—0.138—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliadenosine 5'-triphosphate0.5 M acetate added (stock titrated to assay pH with KOH)—0.088—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliD-glutamic acidno acid added—0.072—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliD-glutamic acid0.5 M formate added (stock titrated to assay pH with KOH)—0.04—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliD-glutamic acid0.5 M acetate added (stock titrated to assay pH with KOH)—0.132—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliUDP-N-acetylmuramoyl-L-alanineno acid added6.670.0051.3 × 10³ calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliUDP-N-acetylmuramoyl-L-alanine0.5 M formate added (stock titrated to assay pH with KOH)1.820.005360 calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)Escherichia coliUDP-N-acetylmuramoyl-L-alanine0.5 M acetate added (stock titrated to assay pH with KOH)1.480.005300 calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliadenosine 5'-triphosphateno acid added—0.215—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliadenosine 5'-triphosphate0.5 M acetate added (stock titrated to assay pH with KOH)—0.216—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliD-glutamic acidno acid added—0.283—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliD-glutamic acid0.5 M acetate added (stock titrated to assay pH with KOH)—0.188—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliUDP-N-acetylmuramoyl-L-alanineno acid added0.01080.1650.0660 calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198AEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.5 M acetate added (stock titrated to assay pH with KOH)0.2820.2561.10 calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliadenosine 5'-triphosphateno acid added—0.119—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliadenosine 5'-triphosphate0.5 M formate added (stock titrated to assay pH with KOH)—0.144—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliD-glutamic acidno acid added—0.688—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliD-glutamic acid0.5 M formate added (stock titrated to assay pH with KOH)—0.485—pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliUDP-N-acetylmuramoyl-L-alanineno acid added0.00730.1220.060 calc.pH 9.4, 37 °COpen
UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (MurD)variant K198FEscherichia coliUDP-N-acetylmuramoyl-L-alanine0.5 M formate added (stock titrated to assay pH with KOH)0.08950.5590.160 calc.pH 9.4, 37 °COpen