Values CatLog read from this paper
Purification and biochemical characterization of Mur ligases from Staphylococcus aureus
PMID 20659527doi:10.1016/j.biochi.2010.07.009Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (13 values) from this paper: MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase), MurC ligase (UDP-MurNAc:L-Ala ligase), MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) and 1 more from Staphylococcus aureus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 6.3.2.7, EC 6.3.2.8, EC 6.3.2.9, EC 6.3.2.10
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MurC ligase (UDP-MurNAc:L-Ala ligase) | Staphylococcus aureus | adenosine 5'-triphosphate | — | 2 | — | pH 8.6, 37 °C | Open record: MurC ligase (UDP-MurNAc:L-Ala ligase), adenosine 5'-triphosphate |
| MurC ligase (UDP-MurNAc:L-Ala ligase) | Staphylococcus aureus | L-alanine | — | 0.44 | — | pH 8.6, 37 °C | Open record: MurC ligase (UDP-MurNAc:L-Ala ligase), L-alanine |
| MurC ligase (UDP-MurNAc:L-Ala ligase) | Staphylococcus aureus | UDP-N-acetylmuramic acid | — | 0.28 | — | pH 8.6, 37 °C | Open record: MurC ligase (UDP-MurNAc:L-Ala ligase), UDP-N-acetylmuramic acid |
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | adenosine 5'-triphosphate | — | 5.4 | — | pH 8.8, 37 °C | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), adenosine 5'-triphosphate |
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | D-glutamic acid | — | 0.13 | — | pH 8.8, 37 °C | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), D-glutamic acid |
| MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase) | Staphylococcus aureus | UDP-N-acetylmuramoyl-L-alanine | — | 0.041 | — | pH 8.8, 37 °C | Open record: MurD ligase (UDP-MurNAc-L-Ala:D-Glu ligase), UDP-N-acetylmuramoyl-L-alanine |
| MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase) | Staphylococcus aureus | adenosine 5'-triphosphate | — | 0.53 | — | pH 8.6, 37 °C | Open record: MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase), adenosine 5'-triphosphate |
| MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase) | Staphylococcus aureus | L-lysine | — | 0.55 | — | pH 8.6, 37 °C | Open record: MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase), L-lysine |
| MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase) | Staphylococcus aureus | L-ornithine | — | 37 | — | pH 8.6, 37 °C | Open record: MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase), L-ornithine |
| MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase) | Staphylococcus aureus | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | — | 0.087 | — | pH 8.6, 37 °C | Open record: MurE ligase (UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase), UDP-N-acetylmuramoyl-L-alanyl-D-glutamate |
| MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase) | Staphylococcus aureus | adenosine 5'-triphosphate | — | 0.11 | — | pH 8.6, 37 °C | Open record: MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase), adenosine 5'-triphosphate |
| MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase) | Staphylococcus aureus | D-alanyl-D-alanine | — | 0.24 | — | pH 8.6, 37 °C | Open record: MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase), D-alanyl-D-alanine |
| MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase) | Staphylococcus aureus | UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine | — | 0.046 | — | pH 8.6, 37 °C | Open record: MurF ligase (UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase), UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine |