Values CatLog read from this paper
Substrate diversity of herpes simplex virus thymidine kinase. Impact Of the kinematics of the enzyme
PMID 10542226doi:10.1074/jbc.274.45.31967Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (18 values) from this paper: thymidine kinase from Herpes simplex virus type 1.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidine kinase | Herpes simplex virus type 1 | 3'-azidodeoxythymidine | 0.056 | 0.0052 | 11 calc. | pH 7.2 | Open record: thymidine kinase, 3'-azidodeoxythymidine |
| thymidine kinase | Herpes simplex virus type 1 | acyclovir | — | 0.2 | — | pH 7.2 | Open record: thymidine kinase, acyclovir |
| thymidine kinase | Herpes simplex virus type 1 | thymidine | 0.35 | 2 × 10⁻⁴ | 1.8 × 10³ calc. | pH 7.2 | Open record: thymidine kinase, thymidine |
| thymidine kinasevariant E225L | Herpes simplex virus type 1 | 3'-azidodeoxythymidine | 0.032 | 0.017 | 1.9 calc. | pH 7.2 | Open record: thymidine kinase variant E225L, 3'-azidodeoxythymidine |
| thymidine kinasevariant E225L | Herpes simplex virus type 1 | thymidine | 0.016 | 0.0123 | 1.3 calc. | pH 7.2 | Open record: thymidine kinase variant E225L, thymidine |
| thymidine kinasevariant H58L | Herpes simplex virus type 1 | thymidine | 0.006 | 0.112 | 0.054 calc. | Open record: thymidine kinase variant H58L, thymidine | |
| thymidine kinasevariant H58L/M128F/Y172F | Herpes simplex virus type 1 | thymidine | 0.04 | 0.183 | 0.22 calc. | Open record: thymidine kinase variant H58L/M128F/Y172F, thymidine | |
| thymidine kinasevariant M128I | Herpes simplex virus type 1 | acyclovir | — | 0.51 | — | pH 7.2 | Open record: thymidine kinase variant M128I, acyclovir |
| thymidine kinasevariant M128I | Herpes simplex virus type 1 | thymidine | 0.51 | 3.6 × 10⁻⁴ | 1.4 × 10³ calc. | pH 7.2 | Open record: thymidine kinase variant M128I, thymidine |
| thymidine kinasevariant Y172F | Herpes simplex virus type 1 | thymidine | 0.46 | 5.1 × 10⁻⁴ | 900 calc. | Open record: thymidine kinase variant Y172F, thymidine |