EC 2.7.1.21: thymidine kinase

TransferasesOpen in Browse

CatLog read 145 records for EC 2.7.1.21 from 14 papers, for 17 organisms. Names in these papers: thymidine kinase, thymidine kinase 1 (CeTK1), thymidine kinase (EBV TK), thymidine kinase (TmTK) and 16 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 80 records for EC 2.7.1.21 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.21 in Browse.

Papers

Papers with records for EC 2.7.1.21
Paper	Organisms	Records
Comparative active-site mutation study of human and Caenorhabditis elegans thymidine kinase 1	Caenorhabditis elegans, Homo sapiens	24
Thymidine kinase 1 regulatory fine-tuning through tetramer formation	Anolis carolinensis, Arabidopsis thaliana and 7 more	20
Characterization of three essential residues in the conserved ATP-binding region of Epstein-Barr virus thymidine kinase	Epstein-Barr virus (human herpesvirus 4)	14
Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes	Thermotoga maritima	14
Trypanosoma brucei thymidine kinase is tandem protein consisting of two homologous parts, which together enable efficient substrate binding	Trypanosoma brucei	14
Characterization of herpes simplex virus type 1 thymidine kinase mutants engineered for improved ganciclovir or acyclovir activity	Herpes Simplex Virus type 1	12
Substrate diversity of herpes simplex virus thymidine kinase. Impact Of the kinematics of the enzyme	Herpes simplex virus type 1	10
Synthesis of N3-substituted carboranyl thymidine bioconjugates and their evaluation as substrates of recombinant human thymidine kinase 1	Homo sapiens	10
Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana	Arabidopsis thaliana	9
Conservative mutations of glutamine-125 in herpes simplex virus type 1 thymidine kinase result in a ganciclovir kinase with minimal deoxypyrimidine kinase activities	Herpes simplex virus type 1	7
Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major	Leishmania major	7
Deoxyribonucleoside kinases activate nucleoside antibiotics in severely pathogenic bacteria	Staphylococcus aureus	2
Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases	Dictyostelium discoideum	1
Identification of key metabolic enzymes involved in the activation of obeldesivir and remdesivir to the active triphosphate metabolite	Homo sapiens	1

Records

Records for EC 2.7.1.21 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)	Herpes Simplex Virus type 1	acyclovir	0.015	0.417	0.036	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK), acyclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)	Herpes Simplex Virus type 1	ganciclovir	0.1	0.0476	2.1	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK), ganciclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)	Herpes Simplex Virus type 1	thymidine	0.46	3.8 × 10⁻⁴	1.2 × 10³	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK), thymidine
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR11	Herpes Simplex Virus type 1	acyclovir	4.7 × 10⁻⁴	0.0056	0.083	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR11, acyclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR11	Herpes Simplex Virus type 1	ganciclovir	0.0056	0.00641	0.87	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR11, ganciclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR11	Herpes Simplex Virus type 1	thymidine	0.007	9.5 × 10⁻⁴	7.3	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR11, thymidine
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR26	Herpes Simplex Virus type 1	acyclovir	2.8 × 10⁻⁴	0.00337	0.083	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR26, acyclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR26	Herpes Simplex Virus type 1	ganciclovir	0.0089	0.01755	0.051	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR26, ganciclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR26	Herpes Simplex Virus type 1	thymidine	0.0062	0.0013	4.8	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR26, thymidine
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR39	Herpes Simplex Virus type 1	acyclovir	4.3 × 10⁻⁴	0.00979	0.044	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR39, acyclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR39	Herpes Simplex Virus type 1	ganciclovir	0.0035	0.00333	1.1	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR39, ganciclovir
Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK)variant SR39	Herpes Simplex Virus type 1	thymidine	0.017	0.00264	6.3	37 °C	PMID 12192082	Open record: Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) variant SR39, thymidine
SaTK (thymidine kinase)	Staphylococcus aureus	3'-azido-3'-deoxythymidine	1.48	0.0116	129	pH 8	PMID 17526755	Open record: SaTK (thymidine kinase), 3'-azido-3'-deoxythymidine
SaTK (thymidine kinase)	Staphylococcus aureus	thymidine	0.44	0.0028	160	pH 8	PMID 17526755	Open record: SaTK (thymidine kinase), thymidine
thymidine kinase	Herpes simplex virus type 1	3'-azidodeoxythymidine	0.056	0.0052	11 calc.	pH 7.2	PMID 10542226	Open record: thymidine kinase, 3'-azidodeoxythymidine
thymidine kinase	Herpes simplex virus type 1	acyclovir	—	0.2	—	pH 7.2	PMID 10542226	Open record: thymidine kinase, acyclovir
thymidine kinase	Herpes simplex virus type 1	thymidine	0.35	2 × 10⁻⁴	1.8 × 10³ calc.	pH 7.2	PMID 10542226	Open record: thymidine kinase, thymidine
thymidine kinasevariant E225L	Herpes simplex virus type 1	3'-azidodeoxythymidine	0.032	0.017	1.9 calc.	pH 7.2	PMID 10542226	Open record: thymidine kinase variant E225L, 3'-azidodeoxythymidine
thymidine kinasevariant E225L	Herpes simplex virus type 1	thymidine	0.016	0.0123	1.3 calc.	pH 7.2	PMID 10542226	Open record: thymidine kinase variant E225L, thymidine
thymidine kinasevariant H58L	Herpes simplex virus type 1	thymidine	0.006	0.112	0.054 calc.		PMID 10542226	Open record: thymidine kinase variant H58L, thymidine
thymidine kinasevariant H58L/M128F/Y172F	Herpes simplex virus type 1	thymidine	0.04	0.183	0.22 calc.		PMID 10542226	Open record: thymidine kinase variant H58L/M128F/Y172F, thymidine
thymidine kinasevariant M128I	Herpes simplex virus type 1	acyclovir	—	0.51	—	pH 7.2	PMID 10542226	Open record: thymidine kinase variant M128I, acyclovir
thymidine kinasevariant M128I	Herpes simplex virus type 1	thymidine	0.51	3.6 × 10⁻⁴	1.4 × 10³ calc.	pH 7.2	PMID 10542226	Open record: thymidine kinase variant M128I, thymidine
thymidine kinasevariant Y172F	Herpes simplex virus type 1	thymidine	0.46	5.1 × 10⁻⁴	900 calc.		PMID 10542226	Open record: thymidine kinase variant Y172F, thymidine
thymidine kinase	Trypanosoma brucei	2'-deoxyadenosine	0.047	8.4	0.0056	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 2'-deoxyadenosine
thymidine kinase	Trypanosoma brucei	2'-deoxyguanosine	0.34	2.6	0.13	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 2'-deoxyguanosine
thymidine kinase	Trypanosoma brucei	2'-deoxyinosine	0.8	2.5	0.32	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 2'-deoxyinosine
thymidine kinase	Trypanosoma brucei	2'-deoxyuridinefull-length TK (allele 1); N-terminal His6-tagged	5.1	0.08	63	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 2'-deoxyuridine
thymidine kinase	Trypanosoma brucei	2'-deoxyuridineexpressed as His6-TrxA fusion	5.3	0.43	12	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 2'-deoxyuridine
thymidine kinase	Trypanosoma brucei	5-fluoro-2'-deoxyuridine	3.6	0.03	120	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, 5-fluoro-2'-deoxyuridine
thymidine kinase	Trypanosoma brucei	adenosine 5'-triphosphatethymidine fixed at 0.5 μM; cosubstrate thymidine (0.5 μM)	—	0.18	—	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, adenosine 5'-triphosphate
thymidine kinase	Trypanosoma brucei	adenosine 5'-triphosphatethymidine fixed at 5 μM; cosubstrate thymidine (5 μM)	—	0.22	—	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, adenosine 5'-triphosphate
thymidine kinase	Trypanosoma brucei	adenosine 5'-triphosphatethymidine fixed at 50 μM; cosubstrate thymidine (50 μM)	3.1	0.21	15	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, adenosine 5'-triphosphate
thymidine kinase	Trypanosoma brucei	adenosine 5'-triphosphatedeoxyinosine fixed at 0.5 mM; full-length TK (allele 1)	—	0.32	—	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, adenosine 5'-triphosphate
thymidine kinase	Trypanosoma brucei	adenosine 5'-triphosphatethymidine fixed at 50 μM; cosubstrate thymidine (50 μM)	3.9	0.098	40	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, adenosine 5'-triphosphate
thymidine kinase	Trypanosoma brucei	thymidinefull-length TK (allele 1); N-terminal His6-tagged	3.1	0.0078	400	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, thymidine
thymidine kinase	Trypanosoma brucei	thymidineexpressed as His6-TrxA fusion	3.5	0.046	76	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, thymidine
thymidine kinase	Trypanosoma brucei	uridine	1.5	7.8	0.19	pH 7.8, 37 °C	PMID 22442154	Open record: thymidine kinase, uridine
thymidine kinase (EBV TK)	Epstein-Barr virus (human herpesvirus 4)	adenosine 5'-triphosphate	0.0281	0.03	0.9369	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK), adenosine 5'-triphosphate
thymidine kinase (EBV TK)	Epstein-Barr virus (human herpesvirus 4)	cytidine 5'-triphosphate	0.01867	0.0492	0.3796	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK), cytidine 5'-triphosphate
thymidine kinase (EBV TK)	Epstein-Barr virus (human herpesvirus 4)	guanosine 5'-triphosphate	0.02127	0.0405	0.525	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK), guanosine 5'-triphosphate
thymidine kinase (EBV TK)	Epstein-Barr virus (human herpesvirus 4)	thymidine 5'-triphosphate	0.01047	0.0719	0.1456	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK), thymidine 5'-triphosphate
thymidine kinase (EBV TK)variant G294A	Epstein-Barr virus (human herpesvirus 4)	adenosine 5'-triphosphate	0.01058	0.0487	0.2174	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant G294A, adenosine 5'-triphosphate
thymidine kinase (EBV TK)variant G294A	Epstein-Barr virus (human herpesvirus 4)	cytidine 5'-triphosphate	0.00882	0.0884	0.0998	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant G294A, cytidine 5'-triphosphate
thymidine kinase (EBV TK)variant G294A	Epstein-Barr virus (human herpesvirus 4)	guanosine 5'-triphosphate	0.00762	0.0687	0.1109	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant G294A, guanosine 5'-triphosphate
thymidine kinase (EBV TK)variant K297R	Epstein-Barr virus (human herpesvirus 4)	adenosine 5'-triphosphate	0.00723	0.0876	0.0825	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant K297R, adenosine 5'-triphosphate
thymidine kinase (EBV TK)variant K297R	Epstein-Barr virus (human herpesvirus 4)	cytidine 5'-triphosphate	0.01419	0.1075	0.132	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant K297R, cytidine 5'-triphosphate
thymidine kinase (EBV TK)variant K297R	Epstein-Barr virus (human herpesvirus 4)	guanosine 5'-triphosphate	0.01849	0.043	0.4299	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant K297R, guanosine 5'-triphosphate
thymidine kinase (EBV TK)variant T298S	Epstein-Barr virus (human herpesvirus 4)	adenosine 5'-triphosphate	0.02405	0.0406	0.5923	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant T298S, adenosine 5'-triphosphate
thymidine kinase (EBV TK)variant T298S	Epstein-Barr virus (human herpesvirus 4)	cytidine 5'-triphosphate	0.01348	0.0565	0.2385	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant T298S, cytidine 5'-triphosphate
thymidine kinase (EBV TK)variant T298S	Epstein-Barr virus (human herpesvirus 4)	guanosine 5'-triphosphate	0.01703	0.0451	0.3775	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant T298S, guanosine 5'-triphosphate
thymidine kinase (EBV TK)variant T298S	Epstein-Barr virus (human herpesvirus 4)	thymidine 5'-triphosphate	0.00737	0.1271	0.058	pH 7.5, 37 °C	PMID 15779905	Open record: thymidine kinase (EBV TK) variant T298S, thymidine 5'-triphosphate
thymidine kinase (HSV-1 TK)	Herpes simplex virus type 1	ganciclovir	0.47	0.069	6.8	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK), ganciclovir
thymidine kinase (HSV-1 TK)	Herpes simplex virus type 1	thymidine	0.06	9 × 10⁻⁴	67	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK), thymidine
thymidine kinase (HSV-1 TK)variant Q125D (Asp-125)	Herpes simplex virus type 1	thymidine	0.11	6	0.018	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK) variant Q125D (Asp-125), thymidine
thymidine kinase (HSV-1 TK)variant Q125E (Glu-125)	Herpes simplex virus type 1	ganciclovir	0.04	0.473	0.082	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK) variant Q125E (Glu-125), ganciclovir
thymidine kinase (HSV-1 TK)variant Q125E (Glu-125)	Herpes simplex virus type 1	thymidine	0.003	0.003	0.844	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK) variant Q125E (Glu-125), thymidine
thymidine kinase (HSV-1 TK)variant Q125N (Asn-125)	Herpes simplex virus type 1	ganciclovir	0.08	0.05	1.7	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK) variant Q125N (Asn-125), ganciclovir
thymidine kinase (HSV-1 TK)variant Q125N (Asn-125)	Herpes simplex virus type 1	thymidine	0.13	0.02	6.5	pH 7.6, 37 °C	PMID 10747801	Open record: thymidine kinase (HSV-1 TK) variant Q125N (Asn-125), thymidine
thymidine kinase (LmTK, type II TK)	Leishmania major	2'-deoxythymidine (thymidine)	2.62	0.0011	2.38 × 10³	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 2'-deoxythymidine (thymidine)
thymidine kinase (LmTK, type II TK)	Leishmania major	2'-deoxyuridine	3.06	0.0111	276	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 2'-deoxyuridine
thymidine kinase (LmTK, type II TK)	Leishmania major	3'-azido-3'-deoxythymidine (zidovudine)	1.97	0.00304	651	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 3'-azido-3'-deoxythymidine (zidovudine)
thymidine kinase (LmTK, type II TK)	Leishmania major	5-bromo-2'-deoxyuridine	3.47	0.00205	1.69 × 10³	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 5-bromo-2'-deoxyuridine
thymidine kinase (LmTK, type II TK)	Leishmania major	5-fluoro-2'-deoxyuridine	4.66	0.00572	814	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 5-fluoro-2'-deoxyuridine
thymidine kinase (LmTK, type II TK)	Leishmania major	5-hydroxymethyl-2'-deoxyuridine	2.82	0.0967	29.1	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), 5-hydroxymethyl-2'-deoxyuridine
thymidine kinase (LmTK, type II TK)	Leishmania major	uridine	0.44	1.462	0.303	pH 7.4, 25 °C	PMID 25978379	Open record: thymidine kinase (LmTK, type II TK), uridine
thymidine kinase (TmTK)	Thermotoga maritima	ATP	0.4	0.04	10	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK), ATP
thymidine kinase (TmTK)	Thermotoga maritima	thymidine	0.3	5 × 10⁻⁴	600	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK), thymidine
thymidine kinase (TmTK)variant G55W	Thermotoga maritima	ATP	0.45	0.112	4	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant G55W, ATP
thymidine kinase (TmTK)variant G55W	Thermotoga maritima	thymidine	0.62	8 × 10⁻⁴	770	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant G55W, thymidine
thymidine kinase (TmTK)variant H53A	Thermotoga maritima	ATP	0.52	0.086	6	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant H53A, ATP
thymidine kinase (TmTK)variant H53A	Thermotoga maritima	thymidine	0.51	0.0012	430	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant H53A, thymidine
thymidine kinase (TmTK)variant L129W	Thermotoga maritima	ATP	1.49	0.046	32	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant L129W, ATP
thymidine kinase (TmTK)variant L129W	Thermotoga maritima	thymidine	1.91	0.003	640	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant L129W, thymidine
thymidine kinase (TmTK)variant V34W	Thermotoga maritima	ATP	0.35	0.023	15	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V34W, ATP
thymidine kinase (TmTK)variant V34W	Thermotoga maritima	thymidine	0.59	9 × 10⁻⁴	630	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V34W, thymidine
thymidine kinase (TmTK)variant V51W	Thermotoga maritima	ATP	0.2	0.035	6	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V51W, ATP
thymidine kinase (TmTK)variant V51W	Thermotoga maritima	thymidine	0.25	7 × 10⁻⁴	340	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V51W, thymidine
thymidine kinase (TmTK)variant V58W	Thermotoga maritima	ATP	0.38	0.029	13	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V58W, ATP
thymidine kinase (TmTK)variant V58W	Thermotoga maritima	thymidine	0.47	5 × 10⁻⁴	960	37 °C	PMID 18073106	Open record: thymidine kinase (TmTK) variant V58W, thymidine
thymidine kinase 1 (AcTK1)	Anolis carolinensis	deoxythymidinestored at -80 °C (-ATP form)	—	0.002	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (AcTK1), deoxythymidine
thymidine kinase 1 (AcTK1)	Anolis carolinensis	deoxythymidinestored at -80 °C (+ATP form)	—	0.0025	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (AcTK1), deoxythymidine
thymidine kinase 1 (BcTK1)	Bacillus cereus	deoxythymidinestored at -80 °C (-ATP form)	—	7 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (BcTK1), deoxythymidine
thymidine kinase 1 (BcTK1)	Bacillus cereus	deoxythymidinestored at -80 °C (+ATP form)	—	6 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (BcTK1), deoxythymidine
thymidine kinase 1 (CeTK1)	Caenorhabditis elegans	3'-azido-3'-deoxythymidine	9.3	0.012	780	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1), 3'-azido-3'-deoxythymidine
thymidine kinase 1 (CeTK1)	Caenorhabditis elegans	deoxyguanosine	—	—	0.082	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1), deoxyguanosine
thymidine kinase 1 (CeTK1)	Caenorhabditis elegans	deoxythymidinestored at -80 °C (-ATP form)	—	9 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (CeTK1), deoxythymidine
thymidine kinase 1 (CeTK1)	Caenorhabditis elegans	deoxythymidinestored at -80 °C (+ATP form)	—	6 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (CeTK1), deoxythymidine
thymidine kinase 1 (CeTK1)	Caenorhabditis elegans	thymidine	6.4	0.0023	2.8 × 10³	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1), thymidine
thymidine kinase 1 (CeTK1)variant L143A	Caenorhabditis elegans	3'-azido-3'-deoxythymidine	—	—	0.49	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant L143A, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (CeTK1)variant L143A	Caenorhabditis elegans	deoxycytidine	—	—	0.0019	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant L143A, deoxycytidine
thymidine kinase 1 (CeTK1)variant L143A	Caenorhabditis elegans	thymidine	4.9	0.336	15	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant L143A, thymidine
thymidine kinase 1 (CeTK1)variant M42I	Caenorhabditis elegans	3'-azido-3'-deoxythymidine	0.81	2.318	0.35	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant M42I, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (CeTK1)variant M42I	Caenorhabditis elegans	deoxycytidine	1.7 × 10⁻⁴	0.0036	0.048	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant M42I, deoxycytidine
thymidine kinase 1 (CeTK1)variant M42I	Caenorhabditis elegans	thymidine	2.8	0.565	5	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant M42I, thymidine
thymidine kinase 1 (CeTK1)variant S182A	Caenorhabditis elegans	3'-azido-3'-deoxythymidine	0.58	0.406	1.4	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182A, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (CeTK1)variant S182A	Caenorhabditis elegans	deoxyguanosine	—	—	0.0066	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182A, deoxyguanosine
thymidine kinase 1 (CeTK1)variant S182A	Caenorhabditis elegans	thymidine	0.74	0.074	10	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182A, thymidine
thymidine kinase 1 (CeTK1)variant S182T	Caenorhabditis elegans	3'-azido-3'-deoxythymidine	1.5	0.018	98	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182T, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (CeTK1)variant S182T	Caenorhabditis elegans	deoxyguanosine	—	—	0.23	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182T, deoxyguanosine
thymidine kinase 1 (CeTK1)variant S182T	Caenorhabditis elegans	thymidine	0.97	0.0013	750	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (CeTK1) variant S182T, thymidine
thymidine kinase 1 (DdTK1)	Dictyostelium discoideum	deoxythymidinestored at -80 °C (-ATP form)	—	0.008	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (DdTK1), deoxythymidine
thymidine kinase 1 (DdTK1)	Dictyostelium discoideum	deoxythymidinestored at -80 °C (+ATP form)	—	0.0024	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (DdTK1), deoxythymidine
thymidine kinase 1 (DdTK1)	Dictyostelium discoideum	thymidine	7.5	0.0051	1.5 × 10³		PMID 17448496	Open record: thymidine kinase 1 (DdTK1), thymidine
thymidine kinase 1 (DrTK1)	Danio rerio	deoxythymidinestored at -80 °C (-ATP form)	—	6 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (DrTK1), deoxythymidine
thymidine kinase 1 (DrTK1)	Danio rerio	deoxythymidinestored at -80 °C (+ATP form)	—	0.0014	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (DrTK1), deoxythymidine
thymidine kinase 1 (GgTK1)	Gallus gallus	deoxythymidinestored at -80 °C (-ATP form)	—	0.012	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (GgTK1), deoxythymidine
thymidine kinase 1 (GgTK1)	Gallus gallus	deoxythymidinestored at -80 °C (+ATP form)	—	0.0012	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (GgTK1), deoxythymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine	0.32	0.0174	18 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine	0.47	0.0563	8.3 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine	0.35	0.0356	9.8 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine	0.44	0.0318	14 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine	0.37	0.0799	4.6 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine	0.39	0.0552	7.1 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-propyltetrazol-5-yl]thymidine	0.34	0.014	24 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-propyltetrazol-5-yl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	3-[1(2)-propyltetrazol-5-ylmethyl]thymidine	0.45	0.0373	12 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
thymidine kinase 1 (hTK1)	Homo sapiens	N5-2OH	0.42	0.0415	10 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), N5-2OH
thymidine kinase 1 (hTK1)	Homo sapiens	thymidine	0.46	0.0049	94 calc.	pH 7.6, 37 °C	PMID 23318906	Open record: thymidine kinase 1 (hTK1), thymidine
thymidine kinase 1 (HuTK1)	Homo sapiens	3'-azido-3'-deoxythymidine	3.5	5.2 × 10⁻⁴	6.7 × 10³	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1), 3'-azido-3'-deoxythymidine
thymidine kinase 1 (HuTK1)variant L124A	Homo sapiens	3'-azido-3'-deoxythymidine	1.4	0.033	44	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant L124A, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (HuTK1)variant L124A	Homo sapiens	thymidine	1.8	0.036	51	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant L124A, thymidine
thymidine kinase 1 (HuTK1)variant M28A	Homo sapiens	3'-azido-3'-deoxythymidine	0.34	0.204	1.7	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant M28A, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (HuTK1)variant M28A	Homo sapiens	thymidine	1.4	0.317	4.5	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant M28A, thymidine
thymidine kinase 1 (HuTK1)variant M28I	Homo sapiens	3'-azido-3'-deoxythymidine	0.24	0.254	0.94	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant M28I, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (HuTK1)variant M28I	Homo sapiens	thymidine	1.5	0.349	4.2	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant M28I, thymidine
thymidine kinase 1 (HuTK1)variant T163S	Homo sapiens	3'-azido-3'-deoxythymidine	1.3	4.3 × 10⁻⁴	3.1 × 10³	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant T163S, 3'-azido-3'-deoxythymidine
thymidine kinase 1 (HuTK1)variant T163S	Homo sapiens	thymidine	2.7	0.059	46	pH 7.5	PMID 22385435	Open record: thymidine kinase 1 (HuTK1) variant T163S, thymidine
thymidine kinase 1 (RnTK1)	Rattus norvegicus	deoxythymidinestored at -80 °C (-ATP form)	—	0.017	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (RnTK1), deoxythymidine
thymidine kinase 1 (RnTK1)	Rattus norvegicus	deoxythymidinestored at -80 °C (+ATP form)	—	3 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (RnTK1), deoxythymidine
thymidine kinase 1 (XtTK1)	Xenopus tropicalis	deoxythymidinestored at -80 °C (-ATP form)	—	0.017	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (XtTK1), deoxythymidine
thymidine kinase 1 (XtTK1)	Xenopus tropicalis	deoxythymidinestored at -80 °C (+ATP form)	—	0.0016	—	pH 8	PMID 23351158	Open record: thymidine kinase 1 (XtTK1), deoxythymidine
thymidine kinase 1a (AtTK1a)	Arabidopsis thaliana	2'-deoxyuridine	1.5	0.015	150		PMID 22897443	Open record: thymidine kinase 1a (AtTK1a), 2'-deoxyuridine
thymidine kinase 1a (AtTK1a)	Arabidopsis thaliana	3'-azido-3'-deoxythymidine	0.13	0.0027	48		PMID 22897443	Open record: thymidine kinase 1a (AtTK1a), 3'-azido-3'-deoxythymidine
thymidine kinase 1a (AtTK1a)	Arabidopsis thaliana	deoxythymidinestored at -80 °C (-ATP form)	—	6 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1a (AtTK1a), deoxythymidine
thymidine kinase 1a (AtTK1a)	Arabidopsis thaliana	deoxythymidinestored at -80 °C (+ATP form)	—	7 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1a (AtTK1a), deoxythymidine
thymidine kinase 1a (AtTK1a)	Arabidopsis thaliana	thymidine	0.48	6.2 × 10⁻⁴	770		PMID 22897443	Open record: thymidine kinase 1a (AtTK1a), thymidine
thymidine kinase 1b (AtTK1b)	Arabidopsis thaliana	2'-deoxyuridine	0.045	0.0066	6.8		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b), 2'-deoxyuridine
thymidine kinase 1b (AtTK1b)	Arabidopsis thaliana	3'-azido-3'-deoxythymidine	0.009	3.8 × 10⁻⁴	22		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b), 3'-azido-3'-deoxythymidine
thymidine kinase 1b (AtTK1b)	Arabidopsis thaliana	thymidine	0.02	0.0014	14		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b), thymidine
thymidine kinase 1b (AtTK1b)variant AtTK1b-dN45	Arabidopsis thaliana	2'-deoxyuridine	0.3	0.0056	53		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b) variant AtTK1b-dN45, 2'-deoxyuridine
thymidine kinase 1b (AtTK1b)variant AtTK1b-dN45	Arabidopsis thaliana	3'-azido-3'-deoxythymidine	0.22	0.0019	120		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b) variant AtTK1b-dN45, 3'-azido-3'-deoxythymidine
thymidine kinase 1b (AtTK1b)variant AtTK1b-dN45	Arabidopsis thaliana	thymidine	0.2	8.4 × 10⁻⁴	240		PMID 22897443	Open record: thymidine kinase 1b (AtTK1b) variant AtTK1b-dN45, thymidine
thymidine kinase 1b (AtTK1b)variant dN45 (AtTK1b-dN45)	Arabidopsis thaliana	deoxythymidinestored at -80 °C (-ATP form)	—	9 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1b (AtTK1b) variant dN45 (AtTK1b-dN45), deoxythymidine
thymidine kinase 1b (AtTK1b)variant dN45 (AtTK1b-dN45)	Arabidopsis thaliana	deoxythymidinestored at -80 °C (+ATP form)	—	9 × 10⁻⁴	—	pH 8	PMID 23351158	Open record: thymidine kinase 1b (AtTK1b) variant dN45 (AtTK1b-dN45), deoxythymidine
thymidine kinase 2 (TK2)	Homo sapiens	thymidine	0.115	0.0081	14.2	pH 7.5	PMID 42363837	Open record: thymidine kinase 2 (TK2), thymidine