Values CatLog read from this paper
Trypanosoma brucei thymidine kinase is tandem protein consisting of two homologous parts, which together enable efficient substrate binding
PMID 22442154doi:10.1074/jbc.m112.340059Open in Browse
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CatLog read 14 records (36 values) from this paper: thymidine kinase from Trypanosoma brucei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidine kinase | Trypanosoma brucei | 2'-deoxyadenosine | 0.047 | 8.4 | 0.0056 | pH 7.8, 37 °C | Open record: thymidine kinase, 2'-deoxyadenosine |
| thymidine kinase | Trypanosoma brucei | 2'-deoxyguanosine | 0.34 | 2.6 | 0.13 | pH 7.8, 37 °C | Open record: thymidine kinase, 2'-deoxyguanosine |
| thymidine kinase | Trypanosoma brucei | 2'-deoxyinosine | 0.8 | 2.5 | 0.32 | pH 7.8, 37 °C | Open record: thymidine kinase, 2'-deoxyinosine |
| thymidine kinase | Trypanosoma brucei | 2'-deoxyuridinefull-length TK (allele 1); N-terminal His6-tagged | 5.1 | 0.08 | 63 | pH 7.8, 37 °C | Open record: thymidine kinase, 2'-deoxyuridine |
| thymidine kinase | Trypanosoma brucei | 2'-deoxyuridineexpressed as His6-TrxA fusion | 5.3 | 0.43 | 12 | pH 7.8, 37 °C | Open record: thymidine kinase, 2'-deoxyuridine |
| thymidine kinase | Trypanosoma brucei | 5-fluoro-2'-deoxyuridine | 3.6 | 0.03 | 120 | pH 7.8, 37 °C | Open record: thymidine kinase, 5-fluoro-2'-deoxyuridine |
| thymidine kinase | Trypanosoma brucei | adenosine 5'-triphosphatethymidine fixed at 0.5 μM; cosubstrate thymidine (0.5 μM) | — | 0.18 | — | pH 7.8, 37 °C | Open record: thymidine kinase, adenosine 5'-triphosphate |
| thymidine kinase | Trypanosoma brucei | adenosine 5'-triphosphatethymidine fixed at 5 μM; cosubstrate thymidine (5 μM) | — | 0.22 | — | pH 7.8, 37 °C | Open record: thymidine kinase, adenosine 5'-triphosphate |
| thymidine kinase | Trypanosoma brucei | adenosine 5'-triphosphatethymidine fixed at 50 μM; cosubstrate thymidine (50 μM) | 3.1 | 0.21 | 15 | pH 7.8, 37 °C | Open record: thymidine kinase, adenosine 5'-triphosphate |
| thymidine kinase | Trypanosoma brucei | adenosine 5'-triphosphatedeoxyinosine fixed at 0.5 mM; full-length TK (allele 1) | — | 0.32 | — | pH 7.8, 37 °C | Open record: thymidine kinase, adenosine 5'-triphosphate |
| thymidine kinase | Trypanosoma brucei | adenosine 5'-triphosphatethymidine fixed at 50 μM; cosubstrate thymidine (50 μM) | 3.9 | 0.098 | 40 | pH 7.8, 37 °C | Open record: thymidine kinase, adenosine 5'-triphosphate |
| thymidine kinase | Trypanosoma brucei | thymidinefull-length TK (allele 1); N-terminal His6-tagged | 3.1 | 0.0078 | 400 | pH 7.8, 37 °C | Open record: thymidine kinase, thymidine |
| thymidine kinase | Trypanosoma brucei | thymidineexpressed as His6-TrxA fusion | 3.5 | 0.046 | 76 | pH 7.8, 37 °C | Open record: thymidine kinase, thymidine |
| thymidine kinase | Trypanosoma brucei | uridine | 1.5 | 7.8 | 0.19 | pH 7.8, 37 °C | Open record: thymidine kinase, uridine |