Values CatLog read from this paper
Conservative mutations of glutamine-125 in herpes simplex virus type 1 thymidine kinase result in a ganciclovir kinase with minimal deoxypyrimidine kinase activities
PMID 10747801doi:10.1021/bi992453qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (21 values) from this paper: thymidine kinase (HSV-1 TK) from Herpes simplex virus type 1.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidine kinase (HSV-1 TK) | Herpes simplex virus type 1 | ganciclovir | 0.47 | 0.069 | 6.8 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK), ganciclovir |
| thymidine kinase (HSV-1 TK) | Herpes simplex virus type 1 | thymidine | 0.06 | 9 × 10⁻⁴ | 67 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK), thymidine |
| thymidine kinase (HSV-1 TK)variant Q125D (Asp-125) | Herpes simplex virus type 1 | thymidine | 0.11 | 6 | 0.018 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK) variant Q125D (Asp-125), thymidine |
| thymidine kinase (HSV-1 TK)variant Q125E (Glu-125) | Herpes simplex virus type 1 | ganciclovir | 0.04 | 0.473 | 0.082 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK) variant Q125E (Glu-125), ganciclovir |
| thymidine kinase (HSV-1 TK)variant Q125E (Glu-125) | Herpes simplex virus type 1 | thymidine | 0.003 | 0.003 | 0.844 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK) variant Q125E (Glu-125), thymidine |
| thymidine kinase (HSV-1 TK)variant Q125N (Asn-125) | Herpes simplex virus type 1 | ganciclovir | 0.08 | 0.05 | 1.7 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK) variant Q125N (Asn-125), ganciclovir |
| thymidine kinase (HSV-1 TK)variant Q125N (Asn-125) | Herpes simplex virus type 1 | thymidine | 0.13 | 0.02 | 6.5 | pH 7.6, 37 °C | Open record: thymidine kinase (HSV-1 TK) variant Q125N (Asn-125), thymidine |