Values CatLog read from this paper
D-ribose-5-phosphate isomerase from spinach: heterologous overexpression, purification, characterization, and site-directed mutagenesis of the recombinant enzyme
PMID 10620366doi:10.1006/abbi.1999.1554Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (17 values) from this paper: ribose-5-phosphate isomerase, ribose-5-phosphate isomerase (E. coli host RPI, partially purified) from Spinacia oleracea, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ribose-5-phosphate isomerase | Spinacia oleracea | D-ribose 5-phosphate | 3.44 × 10³ | 0.63 | 5.46 × 10³ | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase, D-ribose 5-phosphate |
| ribose-5-phosphate isomerase | Spinacia oleracea | D-ribulose 5-phosphate | — | 0.2 | — | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase, D-ribulose 5-phosphate |
| ribose-5-phosphate isomerasevariant D87A | Spinacia oleracea | D-ribose 5-phosphate | 0.0421 | 0.56 | 0.0758 | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase variant D87A, D-ribose 5-phosphate |
| ribose-5-phosphate isomerasevariant D90A | Spinacia oleracea | D-ribose 5-phosphate | 13.2 | 0.52 | 25.2 | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase variant D90A, D-ribose 5-phosphate |
| ribose-5-phosphate isomerasevariant E91A | Spinacia oleracea | D-ribose 5-phosphate | 946 | 1.17 | 810 | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase variant E91A, D-ribose 5-phosphate |
| ribose-5-phosphate isomerasevariant K100A | Spinacia oleracea | D-ribose 5-phosphate | 2.54 | 4.41 | 0.575 | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase variant K100A, D-ribose 5-phosphate |
| ribose-5-phosphate isomerase (E. coli host RPI, partially purified) | Escherichia coli | D-ribose 5-phosphate | — | 0.89 | — | pH 8, 25 °C | Open record: ribose-5-phosphate isomerase (E. coli host RPI, partially purified), D-ribose 5-phosphate |