Values CatLog read from this paper

Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase

PMID 10653632doi:10.1021/bi991489fOpen in Browse

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CatLog read 26 records (78 values) from this paper: chalcone synthase (CHS2) from Medicago sativa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.3.1.74

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
chalcone synthase (CHS2)	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	0.0618	0.0041	15.08	pH 7, 25 °C	Open record: chalcone synthase (CHS2), malonyl-coenzyme A
chalcone synthase (CHS2)	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	0.06817	0.0191	3.57	pH 7, 25 °C	Open record: chalcone synthase (CHS2), malonyl-coenzyme A
chalcone synthase (CHS2)	Medicago sativa	p-coumaroyl-coenzyme A	0.03683	0.0045	8.19	pH 7, 25 °C	Open record: chalcone synthase (CHS2), p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant C164A	Medicago sativa	malonyl-coenzyme A	0.00692	0.0185	0.374	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant C164A, malonyl-coenzyme A
chalcone synthase (CHS2)variant C164S	Medicago sativa	malonyl-coenzyme A	0.00672	0.023	0.292	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant C164S, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215S	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	6.25 × 10⁻⁴	0.104	0.006	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215S, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215S	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	0.001247	0.0546	0.0228	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215S, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215S	Medicago sativa	p-coumaroyl-coenzyme A	4.02 × 10⁻⁴	0.0038	0.106	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215S, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant F215W	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	2.25 × 10⁻⁴	0.107	0.0021	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215W, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215W	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	8.22 × 10⁻⁴	0.124	0.0079	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215W, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215W	Medicago sativa	p-coumaroyl-coenzyme A	3.4 × 10⁻⁴	0.0304	0.0111	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215W, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant F215Y	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	3.35 × 10⁻⁴	0.184	0.0018	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215Y, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215Y	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	0.00285	0.0636	0.0448	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215Y, malonyl-coenzyme A
chalcone synthase (CHS2)variant F215Y	Medicago sativa	p-coumaroyl-coenzyme A	6.47 × 10⁻⁴	0.0183	0.0353	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant F215Y, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant H303Q	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	0.00927	0.0082	1.13	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant H303Q, malonyl-coenzyme A
chalcone synthase (CHS2)variant H303Q	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	0.01097	0.0252	0.435	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant H303Q, malonyl-coenzyme A
chalcone synthase (CHS2)variant H303Q	Medicago sativa	p-coumaroyl-coenzyme A	0.00482	0.0076	0.634	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant H303Q, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant N336A	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	1.32 × 10⁻⁴	0.0419	0.0031	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336A, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336A	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	4.82 × 10⁻⁴	0.0678	0.0071	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336A, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336A	Medicago sativa	p-coumaroyl-coenzyme A	5.28 × 10⁻⁴	0.0058	0.091	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336A, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant N336D	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	1.2 × 10⁻⁵	0.0332	3 × 10⁻⁴	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336D, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336D	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	2.32 × 10⁻⁴	0.159	0.0015	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336D, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336D	Medicago sativa	p-coumaroyl-coenzyme A	2.2 × 10⁻⁵	0.0183	0.0012	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336D, p-coumaroyl-coenzyme A
chalcone synthase (CHS2)variant N336K	Medicago sativa	malonyl-coenzyme Anaringenin (chalcone) formation	4.3 × 10⁻⁵	0.127	3 × 10⁻⁴	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336K, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336K	Medicago sativa	malonyl-coenzyme Aradiometric TLC assay with [14C]malonyl-CoA	2.25 × 10⁻⁴	0.339	7 × 10⁻⁴	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336K, malonyl-coenzyme A
chalcone synthase (CHS2)variant N336K	Medicago sativa	p-coumaroyl-coenzyme A	1.2 × 10⁻⁵	0.0281	4 × 10⁻⁴	pH 7, 25 °C	Open record: chalcone synthase (CHS2) variant N336K, p-coumaroyl-coenzyme A