Values CatLog read from this paper
The bifunctional active site of S-adenosylmethionine synthetase. Roles of the basic residues
PMID 10660564doi:10.1074/jbc.275.6.4060Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (55 values) from this paper: S-adenosylmethionine synthetase (AdoMet synthetase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosylmethionine synthetase (AdoMet synthetase) | Escherichia coli | 3-deazaadenosine 5'-triphosphate | — | 0.29 | — | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase), 3-deazaadenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase) | Escherichia coli | 5′-amino-5′-deoxyadenosine triphosphate | 0.03 | 0.14 | 0.21 calc. | Open record: S-adenosylmethionine synthetase (AdoMet synthetase), 5′-amino-5′-deoxyadenosine triphosphate | |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant H14N | Escherichia coli | L-methionine | — | 0.11 | 0.002 | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant H14N, L-methionine |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant H14N | Escherichia coli | MgATP | 2.3 × 10⁻⁴ | 0.07 | 0.0033 | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant H14N, MgATP |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant H14N | Escherichia coli | tripolyphosphatewithout AdoMet | 0.0038 | 0.165 | 0.023 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant H14N, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant H14N | Escherichia coli | tripolyphosphate | 0.038 | 0.028 | 1.4 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant H14N, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K165M | Escherichia coli | L-methionine | — | 0.24 | 2.5 | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K165M, L-methionine |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K165M | Escherichia coli | MgATP | 0.6 | 0.066 | 9.1 | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K165M, MgATP |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K165M | Escherichia coli | tripolyphosphatewithout AdoMet | 5.9 | 1.9 | 3 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K165M, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K165M | Escherichia coli | tripolyphosphate | 0.98 | 0.375 | 2.6 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K165M, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K245M | Escherichia coli | L-methionine | — | 0.095 | 3.4 × 10⁻⁴ | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K245M, L-methionine |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K245M | Escherichia coli | MgATP | 3.6 × 10⁻⁵ | 0.082 | 4.5 × 10⁻⁴ | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K245M, MgATP |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K245M | Escherichia coli | tripolyphosphate | 0.0077 | 0.31 | 0.25 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K245M, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K265A | Escherichia coli | L-methionine | — | 0.54 | 3.7 × 10⁻⁴ | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K265A, L-methionine |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K265A | Escherichia coli | MgATP | 2 × 10⁻⁴ | 5.2 | 3.7 × 10⁻⁵ | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K265A, MgATP |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K265A | Escherichia coli | tripolyphosphatewithout AdoMet | 0.22 | 0.226 | 0.97 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K265A, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K265A | Escherichia coli | tripolyphosphate | 0.25 | 0.031 | 8.1 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K265A, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K269M | Escherichia coli | L-methionine | — | 7.1 | 1 × 10⁻⁴ | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K269M, L-methionine |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K269M | Escherichia coli | MgATP | 7.1 × 10⁻⁴ | 0.16 | 0.0044 | pH 8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K269M, MgATP |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K269M | Escherichia coli | tripolyphosphatewithout AdoMet | 0.014 | 0.02 | 0.68 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K269M, tripolyphosphate |
| S-adenosylmethionine synthetase (AdoMet synthetase)variant K269M | Escherichia coli | tripolyphosphate | 0.045 | 0.0053 | 8.7 | pH 7.8 | Open record: S-adenosylmethionine synthetase (AdoMet synthetase) variant K269M, tripolyphosphate |