Values CatLog read from this paper
Escherichia coli cytosine deaminase: the kinetics and thermodynamics for binding of cytosine to the apoenzyme and the Zn(2+) holoenzyme are similar
PMID 10669789doi:10.1016/s0167-4838(99)00246-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (34 values) from this paper: cytosine deaminase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cytosine deaminase | Escherichia coli | 2-Thiocytosinepurified from E. coli overexpressing the codBA operon | 22.3 | 0.1 | 220 | pH 7.5, 25 °C | Open record: cytosine deaminase, 2-Thiocytosine |
| cytosine deaminase | Escherichia coli | 2-ThiocytosineZn2+CDase (apoenzyme reconstituted with Zn2+) | 2.27 | 0.109 | 21 | pH 7.5, 25 °C | Open record: cytosine deaminase, 2-Thiocytosine |
| cytosine deaminase | Escherichia coli | 5-Azacytosinepurified from E. coli overexpressing the codBA operon | 2.68 | 0.12 | 22 | pH 7.5, 25 °C | Open record: cytosine deaminase, 5-Azacytosine |
| cytosine deaminase | Escherichia coli | 5-AzacytosineZn2+CDase (apoenzyme reconstituted with Zn2+) | 0.65 | 0.36 | 1.8 | pH 7.5, 25 °C | Open record: cytosine deaminase, 5-Azacytosine |
| cytosine deaminase | Escherichia coli | 5-Fluorocytosinepurified from E. coli overexpressing the codBA operon | 16 | 1.7 | 9 | pH 7.5, 25 °C | Open record: cytosine deaminase, 5-Fluorocytosine |
| cytosine deaminase | Escherichia coli | 5-FluorocytosineZn2+CDase (apoenzyme reconstituted with Zn2+) | 5.5 | 11.2 | 0.49 | pH 7.5, 25 °C | Open record: cytosine deaminase, 5-Fluorocytosine |
| cytosine deaminase | Escherichia coli | 6-Azacytosinepurified from E. coli overexpressing the codBA operon | 186 | 1.6 | 116 | pH 7.5, 25 °C | Open record: cytosine deaminase, 6-Azacytosine |
| cytosine deaminase | Escherichia coli | 6-AzacytosineZn2+CDase (apoenzyme reconstituted with Zn2+) | 5.4 | 1.02 | 5.3 | pH 7.5, 25 °C | Open record: cytosine deaminase, 6-Azacytosine |
| cytosine deaminase | Escherichia coli | Creatininepurified from E. coli overexpressing the codBA operon | — | — | 0.278 | pH 7.5, 25 °C | Open record: cytosine deaminase, Creatinine |
| cytosine deaminase | Escherichia coli | CreatinineZn2+CDase (apoenzyme reconstituted with Zn2+) | — | — | 0.134 | pH 7.5, 25 °C | Open record: cytosine deaminase, Creatinine |
| cytosine deaminase | Escherichia coli | Cytosinepurified from E. coli overexpressing the codBA operon | 185 | 0.2 | 925 | pH 7.5, 25 °C | Open record: cytosine deaminase, Cytosine |
| cytosine deaminase | Escherichia coli | CytosineZn2+CDase (apoenzyme reconstituted with Zn2+) | 26.4 | 0.2 | 130 | pH 7.5, 25 °C | Open record: cytosine deaminase, Cytosine |
| cytosine deaminase | Escherichia coli | cytosine | 6.2 | 0.23 | 27 calc. | pH 7.5, 25 °C | Open record: cytosine deaminase, cytosine |