CatLogenzyme kinetics catalog

EC 3.5.4.1: cytosine deaminase

HydrolasesOpen in Browse

CatLog read 38 records for EC 3.5.4.1 from 4 papers, for 2 organisms. Names in these papers: cytosine deaminase, cytosine deaminase (CDA), cytosine deaminase (bCD), cytosine deaminase (yCD).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 25 records for EC 3.5.4.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.4.1 in Browse.

Papers

PaperOrganismsRecords
Escherichia coli cytosine deaminase: the kinetics and thermodynamics for binding of cytosine to the apoenzyme and the Zn(2+) holoenzyme are similarEscherichia coli13
Three-dimensional structure and catalytic mechanism of cytosine deaminaseEscherichia coli9
Yeast cytosine deaminase mutants with increased thermostability impart sensitivity to 5-fluorocytosineSaccharomyces cerevisiae8
Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo—8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
cytosine deaminaseEscherichia coli2-Thiocytosinepurified from E. coli overexpressing the codBA operon22.30.1220pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli2-ThiocytosineZn2+CDase (apoenzyme reconstituted with Zn2+)2.270.10921pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli5-Azacytosinepurified from E. coli overexpressing the codBA operon2.680.1222pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli5-AzacytosineZn2+CDase (apoenzyme reconstituted with Zn2+)0.650.361.8pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli5-Fluorocytosinepurified from E. coli overexpressing the codBA operon161.79pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli5-FluorocytosineZn2+CDase (apoenzyme reconstituted with Zn2+)5.511.20.49pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli6-Azacytosinepurified from E. coli overexpressing the codBA operon1861.6116pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coli6-AzacytosineZn2+CDase (apoenzyme reconstituted with Zn2+)5.41.025.3pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coliCreatininepurified from E. coli overexpressing the codBA operon——0.278pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coliCreatinineZn2+CDase (apoenzyme reconstituted with Zn2+)——0.134pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coliCytosinepurified from E. coli overexpressing the codBA operon1850.2925pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia coliCytosineZn2+CDase (apoenzyme reconstituted with Zn2+)26.40.2130pH 7.5, 25 °CPMID 10669789Open
cytosine deaminaseEscherichia colicytosine6.20.2327 calc.pH 7.5, 25 °CPMID 10669789Open
cytosine deaminase (bCD)—5-fluorocytosine19.713.765.24PMID 19487291Open
cytosine deaminase (bCD)—cytosine49.680.46106.8PMID 19487291Open
cytosine deaminase (bCD)variant D314E/F316L/D317G—5-fluorocytosine83.686.7312.44PMID 19487291Open
cytosine deaminase (bCD)variant D314E/F316L/D317G—cytosine2.131.651.29PMID 19487291Open
cytosine deaminase (bCD)variant V152A/F316C/D317G—5-fluorocytosine101.712.698.02PMID 19487291Open
cytosine deaminase (bCD)variant V152A/F316C/D317G—cytosine1.694.90.35PMID 19487291Open
cytosine deaminase (bCD)variant V315L/F316V/D317G—5-fluorocytosine115.27.5515.27PMID 19487291Open
cytosine deaminase (bCD)variant V315L/F316V/D317G—cytosine2.983.160.94PMID 19487291Open
cytosine deaminase (CDA)Escherichia coli3-oxauracil2.34.10.56pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)Escherichia colicreatinine5.6250.22pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)Escherichia colicytosine1320.97140pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)Escherichia colicytosine2090.33630pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)Escherichia colicytosine60——30 °CPMID 21545144Open
cytosine deaminase (CDA)Escherichia coliisocytosine5.10.4611pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)variant D313NEscherichia colicytosine0.00751.90.004pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)variant E217AEscherichia coli3-oxauracil0.180.850.21pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (CDA)variant H246QEscherichia colicytosine0.180.250.72pH 8.5, 30 °CPMID 21545144Open
cytosine deaminase (yCD)Saccharomyces cerevisiae5-fluorocytosine18.80.083226pH 7.5PMID 18291415Open
cytosine deaminase (yCD)Saccharomyces cerevisiaecytosine1701.17145pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant A23L/V108I/I140LSaccharomyces cerevisiae5-fluorocytosine29.50.14209pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant A23L/V108I/I140LSaccharomyces cerevisiaecytosine86.20.57153pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant A23L/V108I/I140L/D92ESaccharomyces cerevisiae5-fluorocytosine9.50.068139pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant A23L/V108I/I140L/D92ESaccharomyces cerevisiaecytosine21.80.4745.9pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant D92ESaccharomyces cerevisiae5-fluorocytosine31.70.15216pH 7.5PMID 18291415Open
cytosine deaminase (yCD)variant D92ESaccharomyces cerevisiaecytosine51.80.52100pH 7.5PMID 18291415Open