CatLogenzyme kinetics catalog

Values CatLog read from this paper

Yeast cytosine deaminase mutants with increased thermostability impart sensitivity to 5-fluorocytosine

PMID 18291415doi:10.1016/j.jmb.2008.01.002Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (24 values) from this paper: cytosine deaminase (yCD) from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.4.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytosine deaminase (yCD)Saccharomyces cerevisiae5-fluorocytosine18.80.083226pH 7.5Open
cytosine deaminase (yCD)Saccharomyces cerevisiaecytosine1701.17145pH 7.5Open
cytosine deaminase (yCD)variant A23L/V108I/I140LSaccharomyces cerevisiae5-fluorocytosine29.50.14209pH 7.5Open
cytosine deaminase (yCD)variant A23L/V108I/I140LSaccharomyces cerevisiaecytosine86.20.57153pH 7.5Open
cytosine deaminase (yCD)variant A23L/V108I/I140L/D92ESaccharomyces cerevisiae5-fluorocytosine9.50.068139pH 7.5Open
cytosine deaminase (yCD)variant A23L/V108I/I140L/D92ESaccharomyces cerevisiaecytosine21.80.4745.9pH 7.5Open
cytosine deaminase (yCD)variant D92ESaccharomyces cerevisiae5-fluorocytosine31.70.15216pH 7.5Open
cytosine deaminase (yCD)variant D92ESaccharomyces cerevisiaecytosine51.80.52100pH 7.5Open