Values CatLog read from this paper
Identification by mutagenesis of arginines in the substrate binding site of the porcine NADP-dependent isocitrate dehydrogenase
PMID 10681542doi:10.1074/jbc.275.8.5606Open in Browse
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CatLog read 10 records (20 values) from this paper: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) | Sus scrofa | DL-isocitrate | 30.7 | 0.006 | 5.12 × 10³ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart), DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) | Sus scrofa | NADP+ | — | 0.005 | — | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart), NADP+ |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R101Q | Sus scrofa | DL-isocitrate | 1.32 | 0.106 | 12.5 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R101Q, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R101Q | Sus scrofa | NADP+ | — | 0.005 | — | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R101Q, NADP+ |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R110Q | Sus scrofa | DL-isocitrate | 3.09 | 2.6 | 1.19 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R110Q, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R110Q | Sus scrofa | NADP+ | — | 0.018 | — | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R110Q, NADP+ |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R120Q | Sus scrofa | DL-isocitrate | 27.1 | 0.009 | 3.01 × 10³ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R120Q, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R120Q | Sus scrofa | NADP+ | — | 0.006 | — | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R120Q, NADP+ |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R133Q | Sus scrofa | DL-isocitrate | 16.3 | 0.99 | 16.5 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R133Q, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart)variant R133Q | Sus scrofa | NADP+ | — | 0.011 | — | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase (mitochondrial, pig heart) variant R133Q, NADP+ |