Values CatLog read from this paper
Evaluation by site-directed mutagenesis of aspartic acid residues in the metal site of pig heart NADP-dependent isocitrate dehydrogenase
PMID 10694384doi:10.1021/bi9919753Open in Browse
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CatLog read 10 records (20 values) from this paper: NADP-dependent isocitrate dehydrogenase from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADP-dependent isocitrate dehydrogenase | Sus scrofa | DL-isocitrate | — | 0.0043 | 7.2 × 10³ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenase | Sus scrofa | NADP+ | — | 0.0084 | 3.7 × 10³ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase, NADP+ |
| NADP-dependent isocitrate dehydrogenasevariant D253N | Sus scrofa | DL-isocitrate | — | 0.0024 | 1 × 10⁴ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D253N, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenasevariant D253N | Sus scrofa | NADP+ | — | 0.0098 | 2.6 × 10³ | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D253N, NADP+ |
| NADP-dependent isocitrate dehydrogenasevariant D273N | Sus scrofa | DL-isocitrate | — | 0.012 | 140 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D273N, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenasevariant D273N | Sus scrofa | NADP+ | — | 0.01 | 160 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D273N, NADP+ |
| NADP-dependent isocitrate dehydrogenasevariant D275N | Sus scrofa | DL-isocitrate | — | 0.103 | 0.3 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D275N, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenasevariant D275N | Sus scrofa | NADP+ | — | 0.024 | 1.3 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D275N, NADP+ |
| NADP-dependent isocitrate dehydrogenasevariant D279N | Sus scrofa | DL-isocitrate | — | 0.0014 | 20 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D279N, DL-isocitrate |
| NADP-dependent isocitrate dehydrogenasevariant D279N | Sus scrofa | NADP+ | — | 0.01 | 2.8 | pH 7.4, 25 °C | Open record: NADP-dependent isocitrate dehydrogenase variant D279N, NADP+ |