Values CatLog read from this paper
Do cysteine 230 and lysine 238 of biotin carboxylase play a role in the activation of biotin?
PMID 10747803doi:10.1021/bi992662aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (18 values) from this paper: biotin carboxylase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| biotin carboxylase | Escherichia coli | adenosine 5'-diphosphate | — | 0.19 | — | 25 °C | Open record: biotin carboxylase, adenosine 5'-diphosphate |
| biotin carboxylase | Escherichia coli | carbamoyl phosphate | 0.0052 | 4.84 | 0.0011 calc. | 25 °C | Open record: biotin carboxylase, carbamoyl phosphate |
| biotin carboxylase | Escherichia coli | D-biotin | 1.23 | 122 | 0.0101 calc. | 25 °C | Open record: biotin carboxylase, D-biotin |
| biotin carboxylasevariant C230A | Escherichia coli | adenosine 5'-diphosphate | — | 0.62 | — | 25 °C | Open record: biotin carboxylase variant C230A, adenosine 5'-diphosphate |
| biotin carboxylasevariant C230A | Escherichia coli | adenosine 5'-triphosphate | 0.0017 | 4.23 | 4.0 × 10⁻⁴ calc. | 25 °C | Open record: biotin carboxylase variant C230A, adenosine 5'-triphosphate |
| biotin carboxylasevariant C230A | Escherichia coli | carbamoyl phosphate | 0.007 | 7.94 | 8.8 × 10⁻⁴ calc. | 25 °C | Open record: biotin carboxylase variant C230A, carbamoyl phosphate |
| biotin carboxylasevariant C230A | Escherichia coli | D-biotin | 0.513 | 167 | 0.00307 calc. | 25 °C | Open record: biotin carboxylase variant C230A, D-biotin |
| biotin carboxylasevariant C230A | Escherichia coli | hydrogen carbonate | — | 0.7 | — | 25 °C | Open record: biotin carboxylase variant C230A, hydrogen carbonate |
| biotin carboxylasevariant K238Q | Escherichia coli | adenosine 5'-diphosphate | — | 1.2 | — | 25 °C | Open record: biotin carboxylase variant K238Q, adenosine 5'-diphosphate |
| biotin carboxylasevariant K238Q | Escherichia coli | adenosine 5'-triphosphate | 0.00195 | 6.87 | 2.84 × 10⁻⁴ calc. | 25 °C | Open record: biotin carboxylase variant K238Q, adenosine 5'-triphosphate |
| biotin carboxylasevariant K238Q | Escherichia coli | carbamoyl phosphate | 0.0072 | 3.13 | 0.0023 calc. | 25 °C | Open record: biotin carboxylase variant K238Q, carbamoyl phosphate |