EC 6.3.4.14: biotin carboxylase
LigasesOpen in Browse
CatLog read 45 records for EC 6.3.4.14 from 4 papers, for 1 organism. Names in these papers: biotin carboxylase, biotin carboxylase (BC).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 55 records for EC 6.3.4.14 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.4.14 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Site-directed mutagenesis of ATP binding residues of biotin carboxylase. Insight into the mechanism of catalysis | Escherichia coli | 21 |
| Do cysteine 230 and lysine 238 of biotin carboxylase play a role in the activation of biotin? | Escherichia coli | 11 |
| Structural and biochemical studies on the regulation of biotin carboxylase by substrate inhibition and dimerization | Escherichia coli | 7 |
| Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism | Escherichia coli | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| biotin carboxylase | Escherichia coli | adenosine 5'-diphosphate | — | 0.19 | — | 25 °C | PMID 10747803 | Open record: biotin carboxylase, adenosine 5'-diphosphate |
| biotin carboxylase | Escherichia coli | bicarbonate | — | 1.1 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase, bicarbonate |
| biotin carboxylase | Escherichia coli | bicarbonate | 0.44 | 16.2 | 0.0272 | pH 8.5 | PMID 19213731 | Open record: biotin carboxylase, bicarbonate |
| biotin carboxylase | Escherichia coli | biotin | 0.58 | 35.1 | 0.0166 | pH 7.4 | PMID 19213731 | Open record: biotin carboxylase, biotin |
| biotin carboxylase | Escherichia coli | carbamoyl phosphate | 0.0052 | 4.84 | 0.0011 calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase, carbamoyl phosphate |
| biotin carboxylase | Escherichia coli | D-biotin | 1.23 | 122 | 0.0101 calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase, D-biotin |
| biotin carboxylasevariant C230A | Escherichia coli | adenosine 5'-diphosphate | — | 0.62 | — | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant C230A, adenosine 5'-diphosphate |
| biotin carboxylasevariant C230A | Escherichia coli | adenosine 5'-triphosphate | 0.0017 | 4.23 | 4.0 × 10⁻⁴ calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant C230A, adenosine 5'-triphosphate |
| biotin carboxylasevariant C230A | Escherichia coli | carbamoyl phosphate | 0.007 | 7.94 | 8.8 × 10⁻⁴ calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant C230A, carbamoyl phosphate |
| biotin carboxylasevariant C230A | Escherichia coli | D-biotin | 0.513 | 167 | 0.00307 calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant C230A, D-biotin |
| biotin carboxylasevariant C230A | Escherichia coli | hydrogen carbonate | — | 0.7 | — | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant C230A, hydrogen carbonate |
| biotin carboxylasevariant E276Q | Escherichia coli | adenosine 5'-diphosphate | 0.00145 | 0.42 | 0.0035 | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant E276Q, adenosine 5'-diphosphate |
| biotin carboxylasevariant E276Q | Escherichia coli | adenosine 5'-triphosphate | 0.0012 | 3 | 4 × 10⁻⁴ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant E276Q, adenosine 5'-triphosphate |
| biotin carboxylasevariant E276Q | Escherichia coli | bicarbonate | — | 1.9 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant E276Q, bicarbonate |
| biotin carboxylasevariant E276Q | Escherichia coli | biotin | 0.015 | 137 | 1 × 10⁻⁴ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant E276Q, biotin |
| biotin carboxylasevariant E276Q | Escherichia coli | carbamoyl phosphate | — | 2.3 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant E276Q, carbamoyl phosphate |
| biotin carboxylasevariant E296A | Escherichia coli | bicarbonate | 0.034 | 57.5 | 5.9 × 10⁻⁴ | pH 8.5 | PMID 19213731 | Open record: biotin carboxylase variant E296A, bicarbonate |
| biotin carboxylasevariant E296A | Escherichia coli | biotin | 0.028 | 22.7 | 0.0012 | pH 7.4 | PMID 19213731 | Open record: biotin carboxylase variant E296A, biotin |
| biotin carboxylasevariant H209A | Escherichia coli | adenosine 5'-diphosphate | 0.002 | 0.39 | 0.0052 | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant H209A, adenosine 5'-diphosphate |
| biotin carboxylasevariant H209A | Escherichia coli | adenosine 5'-triphosphate | 7.2 × 10⁻⁴ | 5.7 | 1.3 × 10⁻⁴ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant H209A, adenosine 5'-triphosphate |
| biotin carboxylasevariant H209A | Escherichia coli | bicarbonate | — | 16.7 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant H209A, bicarbonate |
| biotin carboxylasevariant H209A | Escherichia coli | biotin | 0.0415 | 1234 | 3.3 × 10⁻⁵ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant H209A, biotin |
| biotin carboxylasevariant H209A | Escherichia coli | carbamoyl phosphate | — | 7.3 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant H209A, carbamoyl phosphate |
| biotin carboxylasevariant K116A | Escherichia coli | biotin | 0.0172 | 147 | 1.17 × 10⁻⁴ calc. | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K116A, biotin |
| biotin carboxylasevariant K116Q | Escherichia coli | adenosine 5'-diphosphate | 0.00128 | 0.31 | 0.0042 | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K116Q, adenosine 5'-diphosphate |
| biotin carboxylasevariant K116Q | Escherichia coli | adenosine 5'-triphosphate | 0.00125 | 4.2 | 3 × 10⁻⁴ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K116Q, adenosine 5'-triphosphate |
| biotin carboxylasevariant K116Q | Escherichia coli | bicarbonate | — | 1.3 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K116Q, bicarbonate |
| biotin carboxylasevariant K116Q | Escherichia coli | carbamoyl phosphate | — | 5 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K116Q, carbamoyl phosphate |
| biotin carboxylasevariant K159Q | Escherichia coli | adenosine 5'-diphosphate | 0.002 | 0.93 | 0.0022 | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K159Q, adenosine 5'-diphosphate |
| biotin carboxylasevariant K159Q | Escherichia coli | adenosine 5'-triphosphate | 0.00145 | 7.4 | 2 × 10⁻⁴ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K159Q, adenosine 5'-triphosphate |
| biotin carboxylasevariant K159Q | Escherichia coli | bicarbonate | — | 1.8 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K159Q, bicarbonate |
| biotin carboxylasevariant K159Q | Escherichia coli | biotin | 0.0062 | 125 | 5 × 10⁻⁵ | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K159Q, biotin |
| biotin carboxylasevariant K159Q | Escherichia coli | carbamoyl phosphate | — | 3 | — | pH 8, 25 °C | PMID 11346647 | Open record: biotin carboxylase variant K159Q, carbamoyl phosphate |
| biotin carboxylasevariant K238Q | Escherichia coli | adenosine 5'-diphosphate | — | 1.2 | — | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant K238Q, adenosine 5'-diphosphate |
| biotin carboxylasevariant K238Q | Escherichia coli | adenosine 5'-triphosphate | 0.00195 | 6.87 | 2.84 × 10⁻⁴ calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant K238Q, adenosine 5'-triphosphate |
| biotin carboxylasevariant K238Q | Escherichia coli | carbamoyl phosphate | 0.0072 | 3.13 | 0.0023 calc. | 25 °C | PMID 10747803 | Open record: biotin carboxylase variant K238Q, carbamoyl phosphate |
| biotin carboxylasevariant R338A | Escherichia coli | bicarbonate | 0.0019 | 18.9 | 1 × 10⁻⁴ | pH 8.5 | PMID 19213731 | Open record: biotin carboxylase variant R338A, bicarbonate |
| biotin carboxylasevariant R338A | Escherichia coli | biotin | 0.0025 | 41.8 | 6 × 10⁻⁵ | pH 7.4 | PMID 19213731 | Open record: biotin carboxylase variant R338A, biotin |
| biotin carboxylase (BC) | Escherichia coli | adenosine 5'-triphosphate | 0.36 | 0.12 | 3 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), adenosine 5'-triphosphate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate1.5 mM Mg-ATP (fixed; competitive-inhibition series) | 0.31 | 1.9 | 0.162 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate7.5 mM Mg-ATP (fixed; competitive-inhibition series) | 0.36 | 5 | 0.071 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate10 mM Mg-ATP (fixed; competitive-inhibition series) | 0.32 | 6.5 | 0.049 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate20 mM Mg-ATP (fixed; competitive-inhibition series) | 0.3 | 11.4 | 0.026 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonateendogenous bicarbonate in the buffers assumed 1.5 mM | 0.32 | 1.3 | 0.25 calc. | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC)variant R16E | Escherichia coli | adenosine 5'-triphosphate | 0.2 | 0.12 | 1.67 | pH 8, 30 °C | PMID 21592965 | Open record: biotin carboxylase (BC) variant R16E, adenosine 5'-triphosphate |