Values CatLog read from this paper
Structural and biochemical studies on the regulation of biotin carboxylase by substrate inhibition and dimerization
PMID 21592965doi:10.1074/jbc.m111.220517Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (20 values) from this paper: biotin carboxylase (BC) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| biotin carboxylase (BC) | Escherichia coli | adenosine 5'-triphosphate | 0.36 | 0.12 | 3 | pH 8, 30 °C | Open record: biotin carboxylase (BC), adenosine 5'-triphosphate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate1.5 mM Mg-ATP (fixed; competitive-inhibition series) | 0.31 | 1.9 | 0.162 | pH 8, 30 °C | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate7.5 mM Mg-ATP (fixed; competitive-inhibition series) | 0.36 | 5 | 0.071 | pH 8, 30 °C | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate10 mM Mg-ATP (fixed; competitive-inhibition series) | 0.32 | 6.5 | 0.049 | pH 8, 30 °C | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonate20 mM Mg-ATP (fixed; competitive-inhibition series) | 0.3 | 11.4 | 0.026 | pH 8, 30 °C | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC) | Escherichia coli | hydrogencarbonateendogenous bicarbonate in the buffers assumed 1.5 mM | 0.32 | 1.3 | 0.25 calc. | pH 8, 30 °C | Open record: biotin carboxylase (BC), hydrogencarbonate |
| biotin carboxylase (BC)variant R16E | Escherichia coli | adenosine 5'-triphosphate | 0.2 | 0.12 | 1.67 | pH 8, 30 °C | Open record: biotin carboxylase (BC) variant R16E, adenosine 5'-triphosphate |