Values CatLog read from this paper
Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism
PMID 19213731doi:10.1074/jbc.m805783200Open in Browse
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CatLog read 6 records (18 values) from this paper: biotin carboxylase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| biotin carboxylase | Escherichia coli | bicarbonate | 0.44 | 16.2 | 0.0272 | pH 8.5 | Open record: biotin carboxylase, bicarbonate |
| biotin carboxylase | Escherichia coli | biotin | 0.58 | 35.1 | 0.0166 | pH 7.4 | Open record: biotin carboxylase, biotin |
| biotin carboxylasevariant E296A | Escherichia coli | bicarbonate | 0.034 | 57.5 | 5.9 × 10⁻⁴ | pH 8.5 | Open record: biotin carboxylase variant E296A, bicarbonate |
| biotin carboxylasevariant E296A | Escherichia coli | biotin | 0.028 | 22.7 | 0.0012 | pH 7.4 | Open record: biotin carboxylase variant E296A, biotin |
| biotin carboxylasevariant R338A | Escherichia coli | bicarbonate | 0.0019 | 18.9 | 1 × 10⁻⁴ | pH 8.5 | Open record: biotin carboxylase variant R338A, bicarbonate |
| biotin carboxylasevariant R338A | Escherichia coli | biotin | 0.0025 | 41.8 | 6 × 10⁻⁵ | pH 7.4 | Open record: biotin carboxylase variant R338A, biotin |