Values CatLog read from this paper

Site-directed mutagenesis of ATP binding residues of biotin carboxylase. Insight into the mechanism of catalysis

PMID 11346647doi:10.1074/jbc.m101472200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (44 values) from this paper: biotin carboxylase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 6.3.4.14

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
biotin carboxylase	Escherichia coli	bicarbonate	—	1.1	—	pH 8, 25 °C	Open record: biotin carboxylase, bicarbonate
biotin carboxylasevariant E276Q	Escherichia coli	adenosine 5'-diphosphate	0.00145	0.42	0.0035	pH 8, 25 °C	Open record: biotin carboxylase variant E276Q, adenosine 5'-diphosphate
biotin carboxylasevariant E276Q	Escherichia coli	adenosine 5'-triphosphate	0.0012	3	4 × 10⁻⁴	pH 8, 25 °C	Open record: biotin carboxylase variant E276Q, adenosine 5'-triphosphate
biotin carboxylasevariant E276Q	Escherichia coli	bicarbonate	—	1.9	—	pH 8, 25 °C	Open record: biotin carboxylase variant E276Q, bicarbonate
biotin carboxylasevariant E276Q	Escherichia coli	biotin	0.015	137	1 × 10⁻⁴	pH 8, 25 °C	Open record: biotin carboxylase variant E276Q, biotin
biotin carboxylasevariant E276Q	Escherichia coli	carbamoyl phosphate	—	2.3	—	pH 8, 25 °C	Open record: biotin carboxylase variant E276Q, carbamoyl phosphate
biotin carboxylasevariant H209A	Escherichia coli	adenosine 5'-diphosphate	0.002	0.39	0.0052	pH 8, 25 °C	Open record: biotin carboxylase variant H209A, adenosine 5'-diphosphate
biotin carboxylasevariant H209A	Escherichia coli	adenosine 5'-triphosphate	7.2 × 10⁻⁴	5.7	1.3 × 10⁻⁴	pH 8, 25 °C	Open record: biotin carboxylase variant H209A, adenosine 5'-triphosphate
biotin carboxylasevariant H209A	Escherichia coli	bicarbonate	—	16.7	—	pH 8, 25 °C	Open record: biotin carboxylase variant H209A, bicarbonate
biotin carboxylasevariant H209A	Escherichia coli	biotin	0.0415	1234	3.3 × 10⁻⁵	pH 8, 25 °C	Open record: biotin carboxylase variant H209A, biotin
biotin carboxylasevariant H209A	Escherichia coli	carbamoyl phosphate	—	7.3	—	pH 8, 25 °C	Open record: biotin carboxylase variant H209A, carbamoyl phosphate
biotin carboxylasevariant K116A	Escherichia coli	biotin	0.0172	147	1.17 × 10⁻⁴ calc.	pH 8, 25 °C	Open record: biotin carboxylase variant K116A, biotin
biotin carboxylasevariant K116Q	Escherichia coli	adenosine 5'-diphosphate	0.00128	0.31	0.0042	pH 8, 25 °C	Open record: biotin carboxylase variant K116Q, adenosine 5'-diphosphate
biotin carboxylasevariant K116Q	Escherichia coli	adenosine 5'-triphosphate	0.00125	4.2	3 × 10⁻⁴	pH 8, 25 °C	Open record: biotin carboxylase variant K116Q, adenosine 5'-triphosphate
biotin carboxylasevariant K116Q	Escherichia coli	bicarbonate	—	1.3	—	pH 8, 25 °C	Open record: biotin carboxylase variant K116Q, bicarbonate
biotin carboxylasevariant K116Q	Escherichia coli	carbamoyl phosphate	—	5	—	pH 8, 25 °C	Open record: biotin carboxylase variant K116Q, carbamoyl phosphate
biotin carboxylasevariant K159Q	Escherichia coli	adenosine 5'-diphosphate	0.002	0.93	0.0022	pH 8, 25 °C	Open record: biotin carboxylase variant K159Q, adenosine 5'-diphosphate
biotin carboxylasevariant K159Q	Escherichia coli	adenosine 5'-triphosphate	0.00145	7.4	2 × 10⁻⁴	pH 8, 25 °C	Open record: biotin carboxylase variant K159Q, adenosine 5'-triphosphate
biotin carboxylasevariant K159Q	Escherichia coli	bicarbonate	—	1.8	—	pH 8, 25 °C	Open record: biotin carboxylase variant K159Q, bicarbonate
biotin carboxylasevariant K159Q	Escherichia coli	biotin	0.0062	125	5 × 10⁻⁵	pH 8, 25 °C	Open record: biotin carboxylase variant K159Q, biotin
biotin carboxylasevariant K159Q	Escherichia coli	carbamoyl phosphate	—	3	—	pH 8, 25 °C	Open record: biotin carboxylase variant K159Q, carbamoyl phosphate