Values CatLog read from this paper
The allosteric regulation of pyruvate kinase
PMID 10751408doi:10.1074/jbc.m001870200Open in Browse
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CatLog read 15 records (29 values) from this paper: pyruvate kinase (FBP-activated PK, pykI gene product) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate kinase (FBP-activated PK, pykI gene product) | Escherichia coli | adenosine 5'-diphosphate | 132 | 0.3 | 440 | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product), adenosine 5'-diphosphate |
| pyruvate kinase (FBP-activated PK, pykI gene product) | Escherichia coli | phosphoenolpyruvateno FBP | 123 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product), phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product) | Escherichia coli | phosphoenolpyruvate2 mM FBP (allosteric activator) | 160 | 0.08 | 2 × 10³ | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product), phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K382Q | Escherichia coli | adenosine 5'-diphosphate | 110 | 0.28 | 392.9 | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K382Q, adenosine 5'-diphosphate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K382Q | Escherichia coli | phosphoenolpyruvateno FBP | 42 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K382Q, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K382Q | Escherichia coli | phosphoenolpyruvate2 mM FBP (allosteric activator) | 111 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K382Q, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K413Q | Escherichia coli | adenosine 5'-diphosphate | 147 | 0.32 | 459.4 | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K413Q, adenosine 5'-diphosphate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K413Q | Escherichia coli | phosphoenolpyruvateno FBP | 158 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K413Q, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant K413Q | Escherichia coli | phosphoenolpyruvate2 mM FBP (allosteric activator) | 188 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant K413Q, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R271L | Escherichia coli | adenosine 5'-diphosphate | 142 | 0.28 | 507.1 | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R271L, adenosine 5'-diphosphate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R271L | Escherichia coli | phosphoenolpyruvateno FBP | 126 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R271L, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R271L | Escherichia coli | phosphoenolpyruvate2 mM FBP (allosteric activator) | 130 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R271L, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R431E | Escherichia coli | adenosine 5'-diphosphate | 103 | 0.35 | 294.3 | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R431E, adenosine 5'-diphosphate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R431E | Escherichia coli | phosphoenolpyruvateno FBP | 109 | — | — | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R431E, phosphoenolpyruvate |
| pyruvate kinase (FBP-activated PK, pykI gene product)variant R431E | Escherichia coli | phosphoenolpyruvate2 mM FBP (allosteric activator) | 109 | 0.05 | 2.18 × 10³ | pH 7.5, 25 °C | Open record: pyruvate kinase (FBP-activated PK, pykI gene product) variant R431E, phosphoenolpyruvate |