CatLogenzyme kinetics catalog

EC 2.7.1.40: pyruvate kinase

TransferasesOpen in Browse

CatLog read 112 records for EC 2.7.1.40 from 15 papers, for 11 organisms. Names in these papers: pyruvate kinase, cytosolic pyruvate kinase (PKc), pyruvate kinase (FBP-activated PK, pykI gene product), pyruvate kinase M1 isozyme (PK-M1) and 9 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 628 records for EC 2.7.1.40 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.40 in Browse.

Papers

PaperOrganismsRecords
Pyruvate kinase of Trypanosoma brucei: overexpression, purification, and functional characterization of wild-type and mutated enzymeTrypanosoma brucei23
The proton transfer step catalyzed by yeast pyruvate kinaseSaccharomyces cerevisiae16
The allosteric regulation of pyruvate kinaseEscherichia coli15
Purification and characterization of cytosolic pyruvate kinase from Brassica napus (rapeseed) suspension cell cultures: implications for the integration of glycolysis with nitrogen assimilationBrassica napus11
Transcriptional and Biochemical Characterization of Cytosolic Pyruvate Kinases in Arabidopsis thalianaArabidopsis thaliana10
Dominant negative role of the glutamic acid residue conserved in the pyruvate kinase M(1) isozyme in the heterotropic allosteric effect involving fructose-1,6-bisphosphateRattus norvegicus8
Cytosolic pyruvate kinase: subunit composition, activity, and amount in developing castor and soybean seeds, and biochemical characterization of the purified castor seed enzymeRicinus communis8
Structural and kinetic characterization of Trypanosoma congolense pyruvate kinaseTrypanosoma brucei, Trypanosoma congolense6
Pyruvate kinase directly generates GTP in glycolysis, supporting growth and contributing to guanosine toxicityBacillus subtilis4
Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? Testing biochemistrySaccharomyces cerevisiae2
A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymesSaccharomyces cerevisiae2
Allosteric regulation of pyruvate kinase enables efficient and robust gluconeogenesis by preventing metabolic conflicts and carbon overflowBacillus subtilis2
Identification of key metabolic enzymes involved in the activation of obeldesivir and remdesivir to the active triphosphate metaboliteHomo sapiens2
Control of glucose utilization in working perfused rat heartRattus norvegicus2
THE RELATIONSHIPS BETWEEN SUBSTRATES AND ENZYMES OF GLYCOLYSIS IN BRAINMus musculus1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
Cdc19p (PYK)Saccharomyces cerevisiaeADP—0.243—30 °CPMID 23831062Open
Cdc19p (PYK)Saccharomyces cerevisiaephosphoenolpyruvate20.10.28171.5 calc.30 °CPMID 23831062Open
cytosolic pyruvate kinase (PKc)Brassica napusadenosine 5'-diphosphate—0.075—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napuscytidine 5'-diphosphate—4.1—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusinosine 5'-diphosphate—0.68—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvate—0.12—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvatewith 10 mM L-Asp—0.097—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvatewith 10 mM L-Glu—0.2—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvatewith 0.15 mM quercetin—0.33—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvate—0.078—pH 7.4, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvatewith 10 mM L-Asp—0.074—pH 7.4, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusphosphoenolpyruvatewith 10 mM L-Glu—0.28—pH 7.4, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Brassica napusuridine 5'-diphosphate—0.16—pH 6.8, 24 °CPMID 10880971Open
cytosolic pyruvate kinase (PKc)Ricinus communisadenosine 5'-diphosphate—0.09—pH 6.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisadenosine 5'-diphosphatewith 10 mM glutamate—0.101—pH 6.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisadenosine 5'-diphosphate—0.046—pH 7.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisadenosine 5'-diphosphatewith 10 mM glutamate—0.055—pH 7.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisphosphoenolpyruvate—0.082—pH 6.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisphosphoenolpyruvatewith 10 mM glutamate—0.15—pH 6.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisphosphoenolpyruvate—0.046—pH 7.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase (PKc)Ricinus communisphosphoenolpyruvatewith 10 mM glutamate—0.093—pH 7.4, 25 °CPMID 16049677Open
cytosolic pyruvate kinase cPK1Arabidopsis thalianaadenosine 5'-diphosphate—0.15—pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK1Arabidopsis thalianaphosphoenolpyruvate1.530.0626 calc.pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK2Arabidopsis thalianaadenosine 5'-diphosphate—0.03—pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK2Arabidopsis thalianaphosphoenolpyruvate3.050.764.0 calc.pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK3Arabidopsis thalianaadenosine 5'-diphosphate—0.14—pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK3Arabidopsis thalianaphosphoenolpyruvate2.40.1714 calc.pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK4Arabidopsis thalianaadenosine 5'-diphosphate—0.07—pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK4Arabidopsis thalianaphosphoenolpyruvate40.1724 calc.pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK5Arabidopsis thalianaadenosine 5'-diphosphate—0.34—pH 7.5, 25 °CPMID 32168758Open
cytosolic pyruvate kinase cPK5Arabidopsis thalianaphosphoenolpyruvate6.680.05130 calc.pH 7.5, 25 °CPMID 32168758Open
pyruvate kinaseBacillus subtilisadenosine 5'-diphosphate—1.01—pH 7.5PMID 39873491Open
pyruvate kinaseBacillus subtilisadenosine 5'-diphosphate1501.1134pH 7.5, 25 °CPMID 39998177Open
pyruvate kinaseBacillus subtiliscytidine 5'-diphosphate575.610.3pH 7.5, 25 °CPMID 39998177Open
pyruvate kinaseBacillus subtilisguanosine 5'-diphosphate500.0361382pH 7.5, 25 °CPMID 39998177Open
pyruvate kinaseBacillus subtilisuridine 5'-diphosphate1150.35414pH 7.5, 25 °CPMID 39998177Open
pyruvate kinasevariant ΔECTDBacillus subtilisadenosine 5'-diphosphate—1.83—pH 7.5PMID 39873491Open
pyruvate kinaseMus musculusphosphoenolpyruvate—0.055—pH 7.1, 38 °CPMID 14114860Open
pyruvate kinaseRattus norvegicusADP—0.268—pH 7.2, 38 °CPMID 7929251Open
pyruvate kinaseRattus norvegicusphosphoenolpyruvate—0.11—pH 7.2, 38 °CPMID 7929251Open
pyruvate kinaseSaccharomyces cerevisiaeadenosine 5'-diphosphate—0.53—pH 7, 30 °CPMID 10951190Open
pyruvate kinaseSaccharomyces cerevisiaeadenosine 5'-triphosphate10 mM MnCl21.008——pH 7.5PMID 12562754Open
pyruvate kinaseSaccharomyces cerevisiaeadenosine 5'-triphosphate15 mM MgCl20.145——pH 7.5PMID 12562754Open
pyruvate kinaseSaccharomyces cerevisiaephosphoenolpyruvate—0.14—pH 7, 30 °CPMID 10951190Open
pyruvate kinaseSaccharomyces cerevisiaephosphoenolpyruvate4 mM MnCl2 (Mn2+ activator)58.4——pH 6.2PMID 12562754Open
pyruvate kinaseSaccharomyces cerevisiaephosphoenolpyruvate15 mM MgCl2 (Mg2+ activator)232——pH 6.2PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaeadenosine 5'-triphosphate10 mM MnCl20.22——pH 7.5PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaeadenosine 5'-triphosphate15 mM MgCl20.023——pH 7.5PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaephosphoenolpyruvate4 mM MnCl2 (Mn2+ activator)3.30.0482pH 6.2PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaephosphoenolpyruvate4 mM MnCl2 (Mn2+ activator); kcat from a Hill fit, n = 0.74——pH 6.2PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaephosphoenolpyruvate22 mM total MgCl2 (Mg2+ activator)9.4——pH 6.2PMID 12562754Open
pyruvate kinasevariant T298ASaccharomyces cerevisiaephosphoenolpyruvate15 mM MgCl2 (Mg2+ activator)20.90.6632pH 6.2PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaeadenosine 5'-triphosphate10 mM MnCl20.825——pH 7.5PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaeadenosine 5'-triphosphate15 mM MgCl20.138——pH 7.5PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaephosphoenolpyruvate4 mM MnCl2 (Mn2+ activator)35.30.0261.4 × 10³pH 6.2PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaephosphoenolpyruvate4 mM MnCl2 (Mn2+ activator)360.0162.3 × 10³pH 6.2PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaephosphoenolpyruvate15 mM MgCl2 (Mg2+ activator); kcat from a Hill fit, n = 1.964.4——pH 6.2PMID 12562754Open
pyruvate kinasevariant T298SSaccharomyces cerevisiaephosphoenolpyruvate15 mM MgCl2 (Mg2+ activator)77.50.304260pH 6.2PMID 12562754Open
pyruvate kinaseTrypanosoma bruceiadenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b)281.20.1092.58 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinaseTrypanosoma bruceiadenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c)316.30.074.5 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinaseTrypanosoma bruceiphosphoenolpyruvateno effector338——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinaseTrypanosoma bruceiphosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator)341.30.271.3 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinaseTrypanosoma bruceiphosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator)3160.0555.7 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinaseTrypanosoma bruceiphosphoenolpyruvatewith 10 µM fructose 2,6-bisphosphate (allosteric activator)3280.0546.1 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant F463VTrypanosoma bruceiadenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b)168.70.1141.48 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant F463VTrypanosoma bruceiadenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c)228.80.0663.5 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant F463VTrypanosoma bruceiphosphoenolpyruvate340.5——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant L76RTrypanosoma bruceiadenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b)355.3——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant L76RTrypanosoma bruceiadenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c)351.2——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant L76RTrypanosoma bruceiphosphoenolpyruvate357.3——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R19QTrypanosoma bruceiadenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b)2310.1152.01 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R19QTrypanosoma bruceiadenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c)2790.1731.61 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R19QTrypanosoma bruceiphosphoenolpyruvateno effector312.3——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R19QTrypanosoma bruceiphosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator)257——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R19QTrypanosoma bruceiphosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator)267——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiadenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b)42.70.076560 calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiadenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c)1060.0551.9 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiphosphoenolpyruvateno effector225——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiphosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator)225.2——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiphosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator)211.2——pH 7.2, 25 °CPMID 9693062Open
pyruvate kinasevariant R22GTrypanosoma bruceiphosphoenolpyruvatewith 10 µM fructose 2,6-bisphosphate (allosteric activator)157.50.0493.2 × 10³ calc.pH 7.2, 25 °CPMID 9693062Open
pyruvate kinase (FBP-activated PK, pykI gene product)Escherichia coliadenosine 5'-diphosphate1320.3440pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)Escherichia coliphosphoenolpyruvateno FBP123——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)Escherichia coliphosphoenolpyruvate2 mM FBP (allosteric activator)1600.082 × 10³pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K382QEscherichia coliadenosine 5'-diphosphate1100.28392.9pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K382QEscherichia coliphosphoenolpyruvateno FBP42——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K382QEscherichia coliphosphoenolpyruvate2 mM FBP (allosteric activator)111——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K413QEscherichia coliadenosine 5'-diphosphate1470.32459.4pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K413QEscherichia coliphosphoenolpyruvateno FBP158——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant K413QEscherichia coliphosphoenolpyruvate2 mM FBP (allosteric activator)188——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R271LEscherichia coliadenosine 5'-diphosphate1420.28507.1pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R271LEscherichia coliphosphoenolpyruvateno FBP126——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R271LEscherichia coliphosphoenolpyruvate2 mM FBP (allosteric activator)130——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R431EEscherichia coliadenosine 5'-diphosphate1030.35294.3pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R431EEscherichia coliphosphoenolpyruvateno FBP109——pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (FBP-activated PK, pykI gene product)variant R431EEscherichia coliphosphoenolpyruvate2 mM FBP (allosteric activator)1090.052.18 × 10³pH 7.5, 25 °CPMID 10751408Open
pyruvate kinase (TbrPYK)Trypanosoma bruceiphosphoenolpyruvateno effector; kcat from a Hill fit, n = 2.28 ± 0.02104.3——pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase (TbrPYK)Trypanosoma bruceiphosphoenolpyruvatekcat from a Hill fit, n = 1.56 ± 0.02152——pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase (TbrPYK)Trypanosoma bruceiphosphoenolpyruvatekcat from a Hill fit, n = 1.99 ± 0.05117.8——pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase (TcoPYK)Trypanosoma congolensephosphoenolpyruvateno effector; kcat from a Hill fit, n = 1.70 ± 0.0986.8——pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase (TcoPYK)Trypanosoma congolensephosphoenolpyruvatekcat from a Hill fit, n = 1.00 ± 0.07111.50.38306pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase (TcoPYK)Trypanosoma congolensephosphoenolpyruvatekcat from a Hill fit, n = 2.32 ± 0.29107.3——pH 7.2, 25 °CPMID 32084384Open
pyruvate kinase LR (PKLR)Homo sapiensadenosine diphosphate52.950.201263pH 6.5PMID 42363837Open
pyruvate kinase LR (PKLR)Homo sapiensGS-441524 5'-diphosphate0.4350.21862pH 6.5PMID 42363837Open
pyruvate kinase M1 isozyme (PK-M1)Rattus norvegicusadenosine 5'-diphosphate—0.56—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant A398RRattus norvegicusadenosine 5'-diphosphate—0.5—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant A398R/E432ARattus norvegicusadenosine 5'-diphosphate—0.52—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant A398R/E432DRattus norvegicusadenosine 5'-diphosphate—0.45—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant A398R/E432KRattus norvegicusadenosine 5'-diphosphate—0.57—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant E432ARattus norvegicusadenosine 5'-diphosphate—0.7—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant E432DRattus norvegicusadenosine 5'-diphosphate—0.66—pH 7.5, 37 °CPMID 10734049Open
pyruvate kinase M1 isozyme (PK-M1)variant E432KRattus norvegicusadenosine 5'-diphosphate—0.62—pH 7.5, 37 °CPMID 10734049Open