Values CatLog read from this paper
The proton transfer step catalyzed by yeast pyruvate kinase
PMID 12562754doi:10.1074/jbc.m300257200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (26 values) from this paper: pyruvate kinase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate kinase | Saccharomyces cerevisiae | adenosine 5'-triphosphate10 mM MnCl2 | 1.008 | — | — | pH 7.5 | Open record: pyruvate kinase, adenosine 5'-triphosphate |
| pyruvate kinase | Saccharomyces cerevisiae | adenosine 5'-triphosphate15 mM MgCl2 | 0.145 | — | — | pH 7.5 | Open record: pyruvate kinase, adenosine 5'-triphosphate |
| pyruvate kinase | Saccharomyces cerevisiae | phosphoenolpyruvate4 mM MnCl2 (Mn2+ activator) | 58.4 | — | — | pH 6.2 | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinase | Saccharomyces cerevisiae | phosphoenolpyruvate15 mM MgCl2 (Mg2+ activator) | 232 | — | — | pH 6.2 | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | adenosine 5'-triphosphate10 mM MnCl2 | 0.22 | — | — | pH 7.5 | Open record: pyruvate kinase variant T298A, adenosine 5'-triphosphate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | adenosine 5'-triphosphate15 mM MgCl2 | 0.023 | — | — | pH 7.5 | Open record: pyruvate kinase variant T298A, adenosine 5'-triphosphate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | phosphoenolpyruvate4 mM MnCl2 (Mn2+ activator) | 3.3 | 0.04 | 82 | pH 6.2 | Open record: pyruvate kinase variant T298A, phosphoenolpyruvate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | phosphoenolpyruvate4 mM MnCl2 (Mn2+ activator); kcat from a Hill fit, n = 0.7 | 4 | — | — | pH 6.2 | Open record: pyruvate kinase variant T298A, phosphoenolpyruvate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | phosphoenolpyruvate22 mM total MgCl2 (Mg2+ activator) | 9.4 | — | — | pH 6.2 | Open record: pyruvate kinase variant T298A, phosphoenolpyruvate |
| pyruvate kinasevariant T298A | Saccharomyces cerevisiae | phosphoenolpyruvate15 mM MgCl2 (Mg2+ activator) | 20.9 | 0.66 | 32 | pH 6.2 | Open record: pyruvate kinase variant T298A, phosphoenolpyruvate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | adenosine 5'-triphosphate10 mM MnCl2 | 0.825 | — | — | pH 7.5 | Open record: pyruvate kinase variant T298S, adenosine 5'-triphosphate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | adenosine 5'-triphosphate15 mM MgCl2 | 0.138 | — | — | pH 7.5 | Open record: pyruvate kinase variant T298S, adenosine 5'-triphosphate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | phosphoenolpyruvate4 mM MnCl2 (Mn2+ activator) | 35.3 | 0.026 | 1.4 × 10³ | pH 6.2 | Open record: pyruvate kinase variant T298S, phosphoenolpyruvate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | phosphoenolpyruvate4 mM MnCl2 (Mn2+ activator) | 36 | 0.016 | 2.3 × 10³ | pH 6.2 | Open record: pyruvate kinase variant T298S, phosphoenolpyruvate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | phosphoenolpyruvate15 mM MgCl2 (Mg2+ activator); kcat from a Hill fit, n = 1.9 | 64.4 | — | — | pH 6.2 | Open record: pyruvate kinase variant T298S, phosphoenolpyruvate |
| pyruvate kinasevariant T298S | Saccharomyces cerevisiae | phosphoenolpyruvate15 mM MgCl2 (Mg2+ activator) | 77.5 | 0.304 | 260 | pH 6.2 | Open record: pyruvate kinase variant T298S, phosphoenolpyruvate |