Values CatLog read from this paper
Pyruvate kinase of Trypanosoma brucei: overexpression, purification, and functional characterization of wild-type and mutated enzyme
PMID 9693062doi:10.1006/prep.1998.0918Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 23 records (35 values) from this paper: pyruvate kinase from Trypanosoma brucei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate kinase | Trypanosoma brucei | adenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b) | 281.2 | 0.109 | 2.58 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase, adenosine 5'-diphosphate |
| pyruvate kinase | Trypanosoma brucei | adenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c) | 316.3 | 0.07 | 4.5 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase, adenosine 5'-diphosphate |
| pyruvate kinase | Trypanosoma brucei | phosphoenolpyruvateno effector | 338 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinase | Trypanosoma brucei | phosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator) | 341.3 | 0.27 | 1.3 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinase | Trypanosoma brucei | phosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator) | 316 | 0.055 | 5.7 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinase | Trypanosoma brucei | phosphoenolpyruvatewith 10 µM fructose 2,6-bisphosphate (allosteric activator) | 328 | 0.054 | 6.1 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase, phosphoenolpyruvate |
| pyruvate kinasevariant F463V | Trypanosoma brucei | adenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b) | 168.7 | 0.114 | 1.48 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant F463V, adenosine 5'-diphosphate |
| pyruvate kinasevariant F463V | Trypanosoma brucei | adenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c) | 228.8 | 0.066 | 3.5 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant F463V, adenosine 5'-diphosphate |
| pyruvate kinasevariant F463V | Trypanosoma brucei | phosphoenolpyruvate | 340.5 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant F463V, phosphoenolpyruvate |
| pyruvate kinasevariant L76R | Trypanosoma brucei | adenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b) | 355.3 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant L76R, adenosine 5'-diphosphate |
| pyruvate kinasevariant L76R | Trypanosoma brucei | adenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c) | 351.2 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant L76R, adenosine 5'-diphosphate |
| pyruvate kinasevariant L76R | Trypanosoma brucei | phosphoenolpyruvate | 357.3 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant L76R, phosphoenolpyruvate |
| pyruvate kinasevariant R19Q | Trypanosoma brucei | adenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b) | 231 | 0.115 | 2.01 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant R19Q, adenosine 5'-diphosphate |
| pyruvate kinasevariant R19Q | Trypanosoma brucei | adenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c) | 279 | 0.173 | 1.61 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant R19Q, adenosine 5'-diphosphate |
| pyruvate kinasevariant R19Q | Trypanosoma brucei | phosphoenolpyruvateno effector | 312.3 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R19Q, phosphoenolpyruvate |
| pyruvate kinasevariant R19Q | Trypanosoma brucei | phosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator) | 257 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R19Q, phosphoenolpyruvate |
| pyruvate kinasevariant R19Q | Trypanosoma brucei | phosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator) | 267 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R19Q, phosphoenolpyruvate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | adenosine 5'-diphosphate2 mM PEP fixed; ADP varied (Table 6 footnote b) | 42.7 | 0.076 | 560 calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, adenosine 5'-diphosphate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | adenosine 5'-diphosphate5 mM PEP fixed; ADP varied (Table 6 footnote c) | 106 | 0.055 | 1.9 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, adenosine 5'-diphosphate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | phosphoenolpyruvateno effector | 225 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, phosphoenolpyruvate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | phosphoenolpyruvatewith 0.1 µM fructose 2,6-bisphosphate (allosteric activator) | 225.2 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, phosphoenolpyruvate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | phosphoenolpyruvatewith 1 µM fructose 2,6-bisphosphate (allosteric activator) | 211.2 | — | — | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, phosphoenolpyruvate |
| pyruvate kinasevariant R22G | Trypanosoma brucei | phosphoenolpyruvatewith 10 µM fructose 2,6-bisphosphate (allosteric activator) | 157.5 | 0.049 | 3.2 × 10³ calc. | pH 7.2, 25 °C | Open record: pyruvate kinase variant R22G, phosphoenolpyruvate |