Values CatLog read from this paper
Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of…
PMID 10767281doi:10.1074/jbc.m000128200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 34 records (86 values) from this paper: xylanase 10A (Xyl10A), Cex (family 10 xylanase) from Pseudomonas cellulosa, Cellulomonas fimi.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Cex (family 10 xylanase) | Cellulomonas fimi | β-D-cellobiosyl fluoride | — | — | 1.18 | pH 7.2, 37 °C | Open record: Cex (family 10 xylanase), β-D-cellobiosyl fluoride |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | (4-methyl)umbelliferyl-d-glucopyranoside-β(1′,4′)-d-xylopyranoside | 42.68 | 1.1 | 38.8 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), (4-methyl)umbelliferyl-d-glucopyranoside-β(1′,4′)-d-xylopyranoside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | 4-methylumbelliferyl-β-cellobioside | 2.43 | 45 | 0.053 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), 4-methylumbelliferyl-β-cellobioside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylobioside | 70.6 | 0.401 | 172.2 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), 4-methylumbelliferyl-β-xylobioside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylopyranoside | 5.62 | 262 | 0.022 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), 4-methylumbelliferyl-β-xylopyranoside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 2.08 | 41 | 0.05 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 78.75 | 0.53 | 148.6 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | oat spelt xylan | 588.4 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), oat spelt xylan |
| xylanase 10A (Xyl10A) | Pseudomonas cellulosa | β-D-cellobiosyl fluoride | — | — | 0.083 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A), β-D-cellobiosyl fluoride |
| xylanase 10A (Xyl10A)variant L314A | Pseudomonas cellulosa | 4-methylumbelliferyl-β-cellobioside | 1.68 | 28 | 0.06 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A, 4-methylumbelliferyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant L314A | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylobioside | 0.68 | 1.2 | 0.57 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A, 4-methylumbelliferyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant L314A | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 1.52 | 33 | 0.047 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A, 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant L314A | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 0.8 | 1.1 | 0.73 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A, 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant L314A | Pseudomonas cellulosa | oat spelt xylan | 39.92 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A, oat spelt xylan |
| xylanase 10A (Xyl10A)variant L314A/Y87F | Pseudomonas cellulosa | 4-methylumbelliferyl-β-cellobioside | 3.08 | 15 | 0.205 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A/Y87F, 4-methylumbelliferyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant L314A/Y87F | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylobioside | 0.15 | 1.6 | 0.093 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A/Y87F, 4-methylumbelliferyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant L314A/Y87F | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 3.42 | 13 | 0.262 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A/Y87F, 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant L314A/Y87F | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 0.17 | 1.5 | 0.112 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A/Y87F, 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant L314A/Y87F | Pseudomonas cellulosa | oat spelt xylan | 45.75 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant L314A/Y87F, oat spelt xylan |
| xylanase 10A (Xyl10A)variant W313A | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 0.047 | 139 | 3.3 × 10⁻⁴ | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant W313A, 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant W313A | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 0.67 | 5 | 0.135 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant W313A, 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant W313A | Pseudomonas cellulosa | oat spelt xylan | 89.1 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant W313A, oat spelt xylan |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | 4-methylumbelliferyl-β-cellobioside | 3.3 | 21 | 0.157 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, 4-methylumbelliferyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylobioside | 20.75 | 0.52 | 39.9 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, 4-methylumbelliferyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylopyranoside | 3.23 | 271 | 0.012 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, 4-methylumbelliferyl-β-xylopyranoside |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 3.62 | 25 | 0.14 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 7.35 | 0.31 | 23.72 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant Y87A | Pseudomonas cellulosa | oat spelt xylan | 269.1 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87A, oat spelt xylan |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | 4-methylumbelliferyl-β-cellobioside | 2.88 | 21 | 0.138 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, 4-methylumbelliferyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylobioside | 18.25 | 0.43 | 42.45 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, 4-methylumbelliferyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | 4-methylumbelliferyl-β-xylopyranoside | 5.15 | 254 | 0.02 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, 4-methylumbelliferyl-β-xylopyranoside |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | 4-nitrophenyl-β-cellobioside | 2.57 | 17 | 0.152 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, 4-nitrophenyl-β-cellobioside |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | 4-nitrophenyl-β-xylobioside | 19.8 | 0.46 | 43.05 | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, 4-nitrophenyl-β-xylobioside |
| xylanase 10A (Xyl10A)variant Y87F | Pseudomonas cellulosa | oat spelt xylan | 733.0 | — | — | pH 7.2, 37 °C | Open record: xylanase 10A (Xyl10A) variant Y87F, oat spelt xylan |