CatLogenzyme kinetics catalog

EC 3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end)

HydrolasesOpen in Browse

CatLog read 29 records for EC 3.2.1.91 from 4 papers, for 4 organisms. Names in these papers: cellobiohydrolase Cel7A (formerly CBH I), Cel7B (Ma Cel7B cellobiohydrolase), cellobiohydrolase Cel7A (Te Cel7A), Cex (family 10 xylanase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 71 records for EC 3.2.1.91 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.91 in Browse.

Papers

PaperOrganismsRecords
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutantTrichoderma reesei12
Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7BMelanocarpus albomyces10
Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activityTalaromyces emersonii6
Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of…Cellulomonas fimi1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
Cel7B (Ma Cel7B cellobiohydrolase)Melanocarpus albomyces2-chloro-4-nitrophenyl-β-D-lactosideTrichoderma reesei A36 (secreted with own signal sequence)0.01670.620.027pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)Melanocarpus albomyces2-chloro-4-nitrophenyl-β-D-lactosideSaccharomyces cerevisiae INVSc10.01530.80.018pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)Melanocarpus albomyces4-methylumbelliferyl-β-D-lactosideTrichoderma reesei A36 (secreted with own signal sequence)0.1430.230.62pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)Melanocarpus albomyces4-methylumbelliferyl-β-D-lactosideSaccharomyces cerevisiae INVSc10.1070.280.38pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant G4C/M70CMelanocarpus albomyces2-chloro-4-nitrophenyl-β-D-lactoside0.018210.017pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant G4C/M70CMelanocarpus albomyces4-methylumbelliferyl-β-D-lactoside0.0830.30.27pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant S290TMelanocarpus albomyces2-chloro-4-nitrophenyl-β-D-lactoside0.02051.160.017pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant S290TMelanocarpus albomyces4-methylumbelliferyl-β-D-lactoside0.1080.280.38pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant S290T/G4C/M70CMelanocarpus albomyces2-chloro-4-nitrophenyl-β-D-lactoside0.01650.750.022pH 6, 22 °CPMID 19148633Open
Cel7B (Ma Cel7B cellobiohydrolase)variant S290T/G4C/M70CMelanocarpus albomyces4-methylumbelliferyl-β-D-lactoside0.120.30.4pH 6, 22 °CPMID 19148633Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-cellobioside0.0049——pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-cellobioside4.2 × 10⁻⁴——pH 7, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-cellobioside—0.014—pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-cellobioside—0.011—pH 7, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-lactoside0.65——pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)Trichoderma reesei3,4-dinitrophenyl beta-D-lactoside—0.38—pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-cellobioside0.0065——pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-cellobioside0.0054——pH 7, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-cellobioside—0.048—pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-cellobioside—0.161—pH 7, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-lactoside0.052——pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262GTrichoderma reesei3,4-dinitrophenyl beta-D-lactoside—0.543—pH 5, 25 °CPMID 11336632Open
cellobiohydrolase Cel7A (Te Cel7A)Talaromyces emersonii4-methylumbelliferyl-beta-D-lactosideexpressed in S. cerevisiae (glycosylated)0.5180.331.6pH 5, 22 °CPMID 19951999Open
cellobiohydrolase Cel7A (Te Cel7A)Talaromyces emersonii4-methylumbelliferyl-beta-D-lactosideexpressed in S. cerevisiae0.5530.311.8pH 5, 22 °CPMID 19951999Open
cellobiohydrolase Cel7A (Te Cel7A)variant G4C/A72CTalaromyces emersonii4-methylumbelliferyl-beta-D-lactoside0.4950.261.9pH 5, 22 °CPMID 19951999Open
cellobiohydrolase Cel7A (Te Cel7A)variant G4C/A72C/N54C/P191C/T243C/A375C (triple mutant)Talaromyces emersonii4-methylumbelliferyl-beta-D-lactoside0.4450.231.9pH 5, 22 °CPMID 19951999Open
cellobiohydrolase Cel7A (Te Cel7A)variant N54C/P191CTalaromyces emersonii4-methylumbelliferyl-beta-D-lactoside0.5980.212.8pH 5, 22 °CPMID 19951999Open
cellobiohydrolase Cel7A (Te Cel7A)variant T243C/A375CTalaromyces emersonii4-methylumbelliferyl-beta-D-lactoside0.4470.311.4pH 5, 22 °CPMID 19951999Open
Cex (family 10 xylanase)Cellulomonas fimiβ-D-cellobiosyl fluoride——1.18pH 7.2, 37 °CPMID 10767281Open