Values CatLog read from this paper
Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B
PMID 19148633doi:10.1007/s00253-008-1848-9Open in Browse
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CatLog read 10 records (30 values) from this paper: Cel7B (Ma Cel7B cellobiohydrolase) from Melanocarpus albomyces.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Cel7B (Ma Cel7B cellobiohydrolase) | Melanocarpus albomyces | 2-chloro-4-nitrophenyl-β-D-lactosideTrichoderma reesei A36 (secreted with own signal sequence) | 0.0167 | 0.62 | 0.027 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase), 2-chloro-4-nitrophenyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase) | Melanocarpus albomyces | 2-chloro-4-nitrophenyl-β-D-lactosideSaccharomyces cerevisiae INVSc1 | 0.0153 | 0.8 | 0.018 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase), 2-chloro-4-nitrophenyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase) | Melanocarpus albomyces | 4-methylumbelliferyl-β-D-lactosideTrichoderma reesei A36 (secreted with own signal sequence) | 0.143 | 0.23 | 0.62 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase), 4-methylumbelliferyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase) | Melanocarpus albomyces | 4-methylumbelliferyl-β-D-lactosideSaccharomyces cerevisiae INVSc1 | 0.107 | 0.28 | 0.38 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase), 4-methylumbelliferyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant G4C/M70C | Melanocarpus albomyces | 2-chloro-4-nitrophenyl-β-D-lactoside | 0.0182 | 1 | 0.017 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant G4C/M70C, 2-chloro-4-nitrophenyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant G4C/M70C | Melanocarpus albomyces | 4-methylumbelliferyl-β-D-lactoside | 0.083 | 0.3 | 0.27 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant G4C/M70C, 4-methylumbelliferyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant S290T | Melanocarpus albomyces | 2-chloro-4-nitrophenyl-β-D-lactoside | 0.0205 | 1.16 | 0.017 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant S290T, 2-chloro-4-nitrophenyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant S290T | Melanocarpus albomyces | 4-methylumbelliferyl-β-D-lactoside | 0.108 | 0.28 | 0.38 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant S290T, 4-methylumbelliferyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant S290T/G4C/M70C | Melanocarpus albomyces | 2-chloro-4-nitrophenyl-β-D-lactoside | 0.0165 | 0.75 | 0.022 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant S290T/G4C/M70C, 2-chloro-4-nitrophenyl-β-D-lactoside |
| Cel7B (Ma Cel7B cellobiohydrolase)variant S290T/G4C/M70C | Melanocarpus albomyces | 4-methylumbelliferyl-β-D-lactoside | 0.12 | 0.3 | 0.4 | pH 6, 22 °C | Open record: Cel7B (Ma Cel7B cellobiohydrolase) variant S290T/G4C/M70C, 4-methylumbelliferyl-β-D-lactoside |