Values CatLog read from this paper
Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity
PMID 19951999doi:10.1093/protein/gzp072Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: cellobiohydrolase Cel7A (Te Cel7A) from Talaromyces emersonii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cellobiohydrolase Cel7A (Te Cel7A) | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactosideexpressed in S. cerevisiae (glycosylated) | 0.518 | 0.33 | 1.6 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A), 4-methylumbelliferyl-beta-D-lactoside |
| cellobiohydrolase Cel7A (Te Cel7A) | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactosideexpressed in S. cerevisiae | 0.553 | 0.31 | 1.8 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A), 4-methylumbelliferyl-beta-D-lactoside |
| cellobiohydrolase Cel7A (Te Cel7A)variant G4C/A72C | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactoside | 0.495 | 0.26 | 1.9 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A) variant G4C/A72C, 4-methylumbelliferyl-beta-D-lactoside |
| cellobiohydrolase Cel7A (Te Cel7A)variant G4C/A72C/N54C/P191C/T243C/A375C (triple mutant) | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactoside | 0.445 | 0.23 | 1.9 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A) variant G4C/A72C/N54C/P191C/T243C/A375C (triple mutant), 4-methylumbelliferyl-beta-D-lactoside |
| cellobiohydrolase Cel7A (Te Cel7A)variant N54C/P191C | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactoside | 0.598 | 0.21 | 2.8 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A) variant N54C/P191C, 4-methylumbelliferyl-beta-D-lactoside |
| cellobiohydrolase Cel7A (Te Cel7A)variant T243C/A375C | Talaromyces emersonii | 4-methylumbelliferyl-beta-D-lactoside | 0.447 | 0.31 | 1.4 | pH 5, 22 °C | Open record: cellobiohydrolase Cel7A (Te Cel7A) variant T243C/A375C, 4-methylumbelliferyl-beta-D-lactoside |