Values CatLog read from this paper
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant
PMID 11336632doi:10.1042/0264-6021:3560019Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (12 values) from this paper: cellobiohydrolase Cel7A (formerly CBH I) from Trichoderma reesei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | 0.0049 | — | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | 4.2 × 10⁻⁴ | — | — | pH 7, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | — | 0.014 | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | — | 0.011 | — | pH 7, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-lactoside | 0.65 | — | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-lactoside |
| cellobiohydrolase Cel7A (formerly CBH I) | Trichoderma reesei | 3,4-dinitrophenyl beta-D-lactoside | — | 0.38 | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I), 3,4-dinitrophenyl beta-D-lactoside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | 0.0065 | — | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | 0.0054 | — | — | pH 7, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | — | 0.048 | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-cellobioside | — | 0.161 | — | pH 7, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-cellobioside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-lactoside | 0.052 | — | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-lactoside |
| cellobiohydrolase Cel7A (formerly CBH I)variant E223S/A224H/L225V/T226A/D262G | Trichoderma reesei | 3,4-dinitrophenyl beta-D-lactoside | — | 0.543 | — | pH 5, 25 °C | Open record: cellobiohydrolase Cel7A (formerly CBH I) variant E223S/A224H/L225V/T226A/D262G, 3,4-dinitrophenyl beta-D-lactoside |