Values CatLog read from this paper
A second dihydroorotate dehydrogenase (Type A) of the human pathogen Enterococcus faecalis: expression, purification, and steady-state kinetic mechanism
PMID 10775458doi:10.1006/abbi.2000.1769Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (11 values) from this paper: dihydroorotate dehydrogenase A (DHODase A) from Enterococcus faecalis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | 2-methyl-1,4-naphthoquinone | 55.5 | 0.103 | 539 calc. | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), 2-methyl-1,4-naphthoquinone |
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | fumarate | 22 | 0.2 | 110 calc. | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), fumarate |
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | L-dihydroorotateglobal fit to ping-pong equation [2]; cosubstrate menadione | — | 0.115 | — | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), L-dihydroorotate |
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | L-dihydroorotateDHO varied with electron acceptor oxygen fixed at 0.2 mM | 9.4 | 0.0185 | 510 calc. | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), L-dihydroorotate |
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | L-dihydroorotateDHO varied with electron acceptor fumarate fixed at 0.2 mM | 13.5 | 0.0282 | 479 calc. | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), L-dihydroorotate |
| dihydroorotate dehydrogenase A (DHODase A) | Enterococcus faecalis | L-dihydroorotateDHO varied with electron acceptor menadione fixed at 0.2 mM | 29.8 | 0.0595 | 501 calc. | pH 7.5, 25 °C | Open record: dihydroorotate dehydrogenase A (DHODase A), L-dihydroorotate |