CatLogenzyme kinetics catalog

EC 1.3.3.1: dihydroorotate oxidase

OxidoreductasesOpen in Browse

CatLog read 17 records for EC 1.3.3.1 from 2 papers, for 2 organisms. Names in these papers: dihydroorotate dehydrogenase B (DHODB), dihydroorotate dehydrogenase A (DHODase A).

EC 1.3.3.1 is no longer current; BRENDA: “transferred to EC 1.3.98.1”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactisLactococcus lactis11
A second dihydroorotate dehydrogenase (Type A) of the human pathogen Enterococcus faecalis: expression, purification, and steady-state kinetic mechanismEnterococcus faecalis6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalis2-methyl-1,4-naphthoquinone55.50.103539 calc.pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalisfumarate220.2110 calc.pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalisL-dihydroorotateglobal fit to ping-pong equation [2]; cosubstrate menadione—0.115—pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalisL-dihydroorotateDHO varied with electron acceptor oxygen fixed at 0.2 mM9.40.0185510 calc.pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalisL-dihydroorotateDHO varied with electron acceptor fumarate fixed at 0.2 mM13.50.0282479 calc.pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase A (DHODase A)Enterococcus faecalisL-dihydroorotateDHO varied with electron acceptor menadione fixed at 0.2 mM29.80.0595501 calc.pH 7.5, 25 °CPMID 10775458Open
dihydroorotate dehydrogenase B (DHODB)Lactococcus lactisL-dihydroorotate49.30.09550 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)Lactococcus lactisNAD+550.062890 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)Lactococcus lactisNADHstrictly anaerobic (<5 ppm O2, glove box)—0.0037—pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)Lactococcus lactisNADHaerobic; NADH oxidase reaction with atmospheric O2 (258 µM)11.70.00562.1 × 10³ calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)Lactococcus lactisorotate25.70.02131.21 × 10³ calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48ALactococcus lactisL-dihydroorotate0.120.1950.62 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48ELactococcus lactisL-dihydroorotate0.730.3672.0 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48QLactococcus lactisL-dihydroorotate0.110.1310.84 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48QLactococcus lactisNADH2.360.00122.0 × 10³ calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48QLactococcus lactisorotate0.0210.01441.5 calc.pH 8, 25 °CPMID 16624811Open
dihydroorotate dehydrogenase B (DHODB)variant K48RLactococcus lactisL-dihydroorotate0.420.0449.5 calc.pH 8, 25 °CPMID 16624811Open