Values CatLog read from this paper
Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis
PMID 16624811doi:10.1074/jbc.m601417200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (21 values) from this paper: dihydroorotate dehydrogenase B (DHODB) from Lactococcus lactis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydroorotate dehydrogenase B (DHODB) | Lactococcus lactis | L-dihydroorotate | 49.3 | 0.09 | 550 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB), L-dihydroorotate |
| dihydroorotate dehydrogenase B (DHODB) | Lactococcus lactis | NAD+ | 55 | 0.062 | 890 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB), NAD+ |
| dihydroorotate dehydrogenase B (DHODB) | Lactococcus lactis | NADHstrictly anaerobic (<5 ppm O2, glove box) | — | 0.0037 | — | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB), NADH |
| dihydroorotate dehydrogenase B (DHODB) | Lactococcus lactis | NADHaerobic; NADH oxidase reaction with atmospheric O2 (258 µM) | 11.7 | 0.0056 | 2.1 × 10³ calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB), NADH |
| dihydroorotate dehydrogenase B (DHODB) | Lactococcus lactis | orotate | 25.7 | 0.0213 | 1.21 × 10³ calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB), orotate |
| dihydroorotate dehydrogenase B (DHODB)variant K48A | Lactococcus lactis | L-dihydroorotate | 0.12 | 0.195 | 0.62 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48A, L-dihydroorotate |
| dihydroorotate dehydrogenase B (DHODB)variant K48E | Lactococcus lactis | L-dihydroorotate | 0.73 | 0.367 | 2.0 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48E, L-dihydroorotate |
| dihydroorotate dehydrogenase B (DHODB)variant K48Q | Lactococcus lactis | L-dihydroorotate | 0.11 | 0.131 | 0.84 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48Q, L-dihydroorotate |
| dihydroorotate dehydrogenase B (DHODB)variant K48Q | Lactococcus lactis | NADH | 2.36 | 0.0012 | 2.0 × 10³ calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48Q, NADH |
| dihydroorotate dehydrogenase B (DHODB)variant K48Q | Lactococcus lactis | orotate | 0.021 | 0.0144 | 1.5 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48Q, orotate |
| dihydroorotate dehydrogenase B (DHODB)variant K48R | Lactococcus lactis | L-dihydroorotate | 0.42 | 0.044 | 9.5 calc. | pH 8, 25 °C | Open record: dihydroorotate dehydrogenase B (DHODB) variant K48R, L-dihydroorotate |