Values CatLog read from this paper
Probing the active center gorge of acetylcholinesterase by fluorophores linked to substituted cysteines
PMID 10779503doi:10.1074/jbc.m000606200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (24 values) from this paper: acetylcholinesterase (AChE) from Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetylcholinesterase (AChE)variant A262C | Mus musculus | acetylthiocholine | 2.73 × 10³ | 0.059 | 4.7 × 10⁴ | Open record: acetylcholinesterase (AChE) variant A262C, acetylthiocholine | |
| acetylcholinesterase (AChE)variant H287C | Mus musculus | acetylthiocholine | 3 × 10³ | 0.058 | 5.2 × 10⁴ | Open record: acetylcholinesterase (AChE) variant H287C, acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C | Mus musculus | acetylthiocholine | 2.37 × 10³ | 0.065 | 3.7 × 10⁴ | Open record: acetylcholinesterase (AChE) variant Y124C, acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C-N(CH3)3+ (Cys124 modified with MTSET, 2-(trimethylammonium)ethyl methanethiosulfonate) | Mus musculus | acetylthiocholine | 1.85 × 10³ | 1.13 | 1.63 × 10³ | Open record: acetylcholinesterase (AChE) variant Y124C-N(CH3)3+ (Cys124 modified with MTSET, 2-(trimethylammonium)ethyl methanethiosulfonate), acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C-NH3+ (Cys124 modified with MTSEA, 2-aminoethyl methanethiosulfonate) | Mus musculus | acetylthiocholine | 4.35 × 10³ | 2.36 | 1.8 × 10³ | Open record: acetylcholinesterase (AChE) variant Y124C-NH3+ (Cys124 modified with MTSEA, 2-aminoethyl methanethiosulfonate), acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C-SO3− (Cys124 modified with MTSES, sodium (2-sulfonatoethyl)methanethiosulfonate) | Mus musculus | acetylthiocholine | 2.13 × 10³ | 0.058 | 3.7 × 10⁴ | Open record: acetylcholinesterase (AChE) variant Y124C-SO3− (Cys124 modified with MTSES, sodium (2-sulfonatoethyl)methanethiosulfonate), acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C-acrylodan (Cys124 conjugated with acrylodan) | Mus musculus | acetylthiocholine | 95 | 0.28 | 330 | Open record: acetylcholinesterase (AChE) variant Y124C-acrylodan (Cys124 conjugated with acrylodan), acetylthiocholine | |
| acetylcholinesterase (AChE)variant Y124C-benzyl (Cys124 modified with benzyl methanethiosulfonate, MTSBn) | Mus musculus | acetylthiocholine | 1.33 × 10³ | 0.059 | 2.3 × 10⁴ | Open record: acetylcholinesterase (AChE) variant Y124C-benzyl (Cys124 modified with benzyl methanethiosulfonate, MTSBn), acetylthiocholine |