Values CatLog read from this paper

Catalytic cysteine of thymidylate synthase is activated upon substrate binding

PMID 10841779doi:10.1021/bi000367gOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (25 values) from this paper: thymidylate synthase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.45

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
thymidylate synthase	Escherichia coli	2'-deoxyuridine 5'-monophosphate	2.1	0.0011	1.9 × 10³ calc.	pH 7.5, 27 °C	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0081	—	pH 7.5, 27 °C	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0085	—		Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.099	0.0097	10 calc.	25 °C	Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167A	Escherichia coli	2'-deoxyuridine 5'-monophosphate	1.9	0.0053	360 calc.	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167A	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.012	—	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167A	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0097	—		Open record: thymidylate synthase variant S167A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167A	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.078	0.0723	1.1 calc.	25 °C	Open record: thymidylate synthase variant S167A, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167C	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.022	0.0981	0.22 calc.	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167C	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.13	—	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167C, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.39	0.0339	12 calc.	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167G	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0424	—	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167G, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0426	—		Open record: thymidylate synthase variant S167G, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0019	0.514	0.0037 calc.	25 °C	Open record: thymidylate synthase variant S167G, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167T	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.32	0.0417	7.7 calc.	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167T	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0627	—	pH 7.5, 27 °C	Open record: thymidylate synthase variant S167T, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167T	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0521	—		Open record: thymidylate synthase variant S167T, 5,10-methylenetetrahydrofolate