EC 2.1.1.45: thymidylate synthase

TransferasesOpen in Browse

CatLog read 292 records for EC 2.1.1.45 from 15 papers, for 6 organisms. Names in these papers: thymidylate synthase, thymidylate synthase (TS), dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase), dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 115 records for EC 2.1.1.45 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.45 in Browse.

Papers

Papers with records for EC 2.1.1.45
Paper	Organisms	Records
Replacement set mutagenesis of the four phosphate-binding arginine residues of thymidylate synthase	Lactobacillus casei	105
Complete replacement set of amino acids at the C-terminus of thymidylate synthase: quantitative structure-activity relationship of mutants of an enzyme	Lactobacillus casei	46
Asparagine 229 in thymidylate synthase contributes to, but is not essential for, catalysis	Lactobacillus casei	44
Catalytic cysteine of thymidylate synthase is activated upon substrate binding	Escherichia coli	17
Properties of bacteriophage T4 thymidylate synthase following mutagenic changes in the active site and folate binding region	bacteriophage T4	14
A novel dCMP methylase by engineering thymidylate synthase	Lactobacillus casei	13
Partial restoration of activity to Lactobacillus casei thymidylate synthase following inactivation by domain deletion	Lactobacillus casei	12
Site-directed mutagenesis of arginine 179 of thymidylate synthase. A nonessential substrate-binding residue	Lactobacillus casei	10
Pyrimethamine-resistant dihydrofolate reductase enzymes of Plasmodium falciparum are not enzymatically compromised in vitro	Plasmodium falciparum	8
Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase	Lactobacillus casei	6
Variants of human thymidylate synthase with loop 181-197 stabilized in the inactive conformation	Homo sapiens	5
Conservation and Role of Electrostatics in Thymidylate Synthase	Escherichia coli	4
Isolation and characterization of a mutant dihydrofolate reductase-thymidylate synthase from methotrexate-resistant Leishmania cells	Leishmania major	4
Kinetic properties of human thymidylate synthase, an anticancer drug target	Homo sapiens	3
The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major	Leishmania major	1

Records

Records for EC 2.1.1.45 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)	Plasmodium falciparum	7,8-dihydrofolate	1.24	0.0444	28	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase), 7,8-dihydrofolate
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)	Plasmodium falciparum	NADPH	—	0.0232	—	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase), NADPH
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N	Plasmodium falciparum	7,8-dihydrofolate	1.23	0.0209	59	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N, 7,8-dihydrofolate
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N	Plasmodium falciparum	NADPH	—	0.0379	—	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N, NADPH
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/164L	Plasmodium falciparum	7,8-dihydrofolate	1.42	0.0207	69	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N/164L, 7,8-dihydrofolate
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/164L	Plasmodium falciparum	NADPH	—	0.0373	—	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N/164L, NADPH
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/213A	Plasmodium falciparum	7,8-dihydrofolate	0.68	0.0102	66	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N/213A, 7,8-dihydrofolate
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/213A	Plasmodium falciparum	NADPH	—	0.0309	—	pH 7, 25 °C	PMID 17451822	Open record: dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase) variant 51I/59R/108N/213A, NADPH
dihydrofolate reductase-thymidylate synthase (DHFR-TS)	Leishmania major	folate	—	0.0041	—	pH 5	PMID 9153248	Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), folate
dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity	Leishmania major	(6R,S)-5,10-methylenetetrahydrofolate	—	0.079	—	25 °C	PMID 8144647	Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity, (6R,S)-5,10-methylenetetrahydrofolate
dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity	Leishmania major	2'-deoxyuridine 5'-monophosphate	5.8	0.007	830 calc.	25 °C	PMID 8144647	Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity, 2'-deoxyuridine 5'-monophosphate
dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activityvariant M53R	Leishmania major	(6R,S)-5,10-methylenetetrahydrofolate	—	0.096	—	25 °C	PMID 8144647	Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity variant M53R, (6R,S)-5,10-methylenetetrahydrofolate
dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activityvariant M53R	Leishmania major	2'-deoxyuridine 5'-monophosphate	5.5	0.0065	850 calc.	25 °C	PMID 8144647	Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity variant M53R, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	11.8	0.00273	4.32 × 10³	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.0499	237	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant C156S	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	0.008	0.00492	2	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant C156S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant C156S	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.0819	0.1	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant C156S, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant H157V	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	2.7	0.00412	655	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant H157V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant H157V	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.0752	36	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant H157V, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant K48R	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	0.76	0.0059	128	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant K48R, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant K48R	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.187	4	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant K48R, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant K49R	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	6.65	0.00649	1.04 × 10³	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant K49R, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant K49R	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.0779	85	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant K49R, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant P155A	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	10.3	0.0118	873	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant P155A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant P155A	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.0991	104	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant P155A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant R137K	bacteriophage T4	2'-deoxyuridine 5'-monophosphate	3.8	0.00702	541	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant R137K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R137K	bacteriophage T4	5,10-methylenetetrahydrofolate	—	0.108	35	pH 7, 30 °C	PMID 2271601	Open record: thymidylate synthase variant R137K, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	2'-deoxyuridine 5'-monophosphate	2.1	0.0011	1.9 × 10³ calc.	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Escherichia coli	2'-deoxyuridine 5'-monophosphateindependent experiment set ¶ of Table 3	0.32	0.082	4	pH 7.4, 25 °C	PMID 26612036	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Escherichia coli	2'-deoxyuridine 5'-monophosphateindependent experiment set ║ of Table 3	0.6	0.058	10	pH 7.4, 25 °C	PMID 26612036	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0081	—	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0085	—		PMID 10841779	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolateindependent experiment set ¶ of Table 3	0.28	0.077	4	pH 7.4, 25 °C	PMID 26612036	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5,10-methylenetetrahydrofolateindependent experiment set ║ of Table 3	0.48	0.059	8	pH 7.4, 25 °C	PMID 26612036	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.099	0.0097	10 calc.	25 °C	PMID 10841779	Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167A	Escherichia coli	2'-deoxyuridine 5'-monophosphate	1.9	0.0053	360 calc.	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167A	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.012	—	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167A	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0097	—		PMID 10841779	Open record: thymidylate synthase variant S167A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167A	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.078	0.0723	1.1 calc.	25 °C	PMID 10841779	Open record: thymidylate synthase variant S167A, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167C	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.022	0.0981	0.22 calc.	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167C	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.13	—	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167C, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.39	0.0339	12 calc.	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167G	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0424	—	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167G, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0426	—		PMID 10841779	Open record: thymidylate synthase variant S167G, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167G	Escherichia coli	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0019	0.514	0.0037 calc.	25 °C	PMID 10841779	Open record: thymidylate synthase variant S167G, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167T	Escherichia coli	2'-deoxyuridine 5'-monophosphate	0.32	0.0417	7.7 calc.	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant S167T	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0627	—	pH 7.5, 27 °C	PMID 10841779	Open record: thymidylate synthase variant S167T, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant S167T	Escherichia coli	5,10-methylenetetrahydrofolate	—	0.0521	—		PMID 10841779	Open record: thymidylate synthase variant S167T, 5,10-methylenetetrahydrofolate
thymidylate synthase	Homo sapiens	2'-deoxyuridine 5'-monophosphate	0.213	0.0027	79	pH 7.5, 30 °C	PMID 12859954	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Homo sapiens	2'-deoxyuridine 5'-monophosphate	3.11	0.00259	1.2 × 10³	37 °C	PMID 19569192	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Homo sapiens	5,10-methylenetetrahydrofolate	—	0.0101	—	pH 7.5, 30 °C	PMID 12859954	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Homo sapiens	E-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate	0.0057	0.0116	0.4914	pH 7.5	PMID 12859954	Open record: thymidylate synthase, E-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant A191K	Homo sapiens	2'-deoxyuridine 5'-monophosphate	0.52	8.5 × 10⁻⁴	610	37 °C	PMID 19569192	Open record: thymidylate synthase variant A191K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant L198P	Homo sapiens	2'-deoxyuridine 5'-monophosphate	1.64	0.01563	100	37 °C	PMID 19569192	Open record: thymidylate synthase variant L198P, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant M190E	Homo sapiens	2'-deoxyuridine 5'-monophosphate	0.53	9.6 × 10⁻⁴	550	37 °C	PMID 19569192	Open record: thymidylate synthase variant M190E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant M190K	Homo sapiens	2'-deoxyuridine 5'-monophosphate	0.03	0.179	0.16	37 °C	PMID 19569192	Open record: thymidylate synthase variant M190K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.02	280	25 °C	PMID 10964985	Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.02	360	25 °C	PMID 10964985	Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.02	260	25 °C	PMID 10964985	Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	3.6	0.005	710	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0029	1.9 × 10³	25 °C	PMID 1627545	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	7.8	0.0027	2.9 × 10³		PMID 2182628	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	3.6	0.005	720	pH 7.4	PMID 8378336	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	5.1	0.0026	2 × 10³	25 °C	PMID 10964985	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	7.2	0.003	2.4 × 10³	25 °C	PMID 10964985	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	5.2	0.0028	1.9 × 10³	25 °C	PMID 10964985	Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.01	360	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.01	360	pH 7.4	PMID 8378336	Open record: thymidylate synthase, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase	Lactobacillus casei	5,10-methylenetetrahydrofolate	5.5	0.014	400	25 °C	PMID 1627545	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Lactobacillus casei	5,10-methylenetetrahydrofolate	—	0.02	390		PMID 2182628	Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate
thymidylate synthase	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.013	0.012	1.1 calc.		PMID 1627545	Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.011	0.009	1.2 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0015	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229C	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.22	0.017	13	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229C	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.23	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229C	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.092	2.4	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229C, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229D	Lactobacillus casei	2'-deoxycytidine 5'-monophosphate	0.45	0.15	3	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase variant N229D, 2'-deoxycytidine 5'-monophosphate
thymidylate synthasevariant N229D	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0037	0.0504	0.073	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase variant N229D, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229D	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.0037	0.05	0.074	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229D, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229D	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0041	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229D, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolateTS reaction (dTMP formation); cosubstrate dUMP	—	0.0354	0.01	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase variant N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolatedCMP methylase reaction (m5dCMP formation); cosubstrate dCMP	—	0.17	2.6	pH 7.4, 20 °C	PMID 1606134	Open record: thymidylate synthase variant N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.035	0.11	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.0043	0.066	0.065	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0045	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229E	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.08	0.054	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229E, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229G	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.028	0.015	1.9	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229G	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.03	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229G	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.098	0.29	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229G, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229I	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	1.1	0.149	7.4	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229I, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229I	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.3	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229I, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229I	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.089	12	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229I, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0078	0.0068	1.1	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229K	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.019	0.41	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229K, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	1.1	0.223	4.9	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.4	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229L	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.09	12	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229L, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229M	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.62	0.154	4	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229M, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229M	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.71	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229M, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229M	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.084	7.4	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229M, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.017	0.011	1.5	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.018	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229Q	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.038	0.45	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Q, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.0093	0.039	0.24	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.011	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229S	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.015	0.62	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229S, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229T	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.077	0.104	0.74	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229T	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.084	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229T	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.066	1.2	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229T, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229V	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.86	0.091	9.5	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229V	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.95	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229V	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.057	15	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229V, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229W	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	1.2	0.576	2.1	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229W, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229W	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.8	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229W, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229W	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.173	6.9	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229W, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant N229Y	Lactobacillus casei	2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit	0.0087	0.014	0.62	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Y, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229Y	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0093	—	—	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Y, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant N229Y	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.009	0.97	pH 7.4	PMID 8378336	Open record: thymidylate synthase variant N229Y, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R178A	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.41	5.9	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R178A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.4	5.5	0.44	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R178C	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.22	14.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178C, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R178C	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	3.1	3.9	0.79	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R178K	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.06	133	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R178K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	8	0.13	61.54	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R178S	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.31	7.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R178S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.2	4.5	0.49	25 °C	PMID 10964985	Open record: thymidylate synthase variant R178S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179A	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.01	480	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.4	0.0052	460		PMID 2182628	Open record: thymidylate synthase variant R179A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	4.8	0.0068	710	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179A	Lactobacillus casei	5,10-methylenetetrahydrofolate	—	0.02	120		PMID 2182628	Open record: thymidylate synthase variant R179A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant R179A	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0035	0.09	0.039 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179A, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179C	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.026	73	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179C, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179C	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.6	0.0055	470	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179C	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.003	0.053	0.057 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179C, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179D	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.037	57	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179D, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179D	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.9	0.0028	680	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179D, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179E	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.013	190	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179E, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.9	0.017	110		PMID 2182628	Open record: thymidylate synthase variant R179E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.5	0.02	130	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179E	Lactobacillus casei	5,10-methylenetetrahydrofolate	—	0.016	120		PMID 2182628	Open record: thymidylate synthase variant R179E, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant R179F	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.031	32	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179F, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179F	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.4	0.0027	520	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179F, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179F	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	6 × 10⁻⁴	0.036	0.017 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179F, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179G	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.028	140	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179G, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179G	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	3.2	0.0042	760	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179G	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	9 × 10⁻⁴	0.074	0.012 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179G, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179H	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.048	40	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179H, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179H	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.6	0.0091	290	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179H, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179H	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0029	0.085	0.034 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179H, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179K	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.021	150	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2	0.038	50		PMID 2182628	Open record: thymidylate synthase variant R179K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	3.2	0.008	400	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179K	Lactobacillus casei	5,10-methylenetetrahydrofolate	—	0.066	30		PMID 2182628	Open record: thymidylate synthase variant R179K, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant R179K	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.003	0.061	0.049 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179K, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179L	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.051	33	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179L, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.3	0.0058	400	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179L	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.001	0.022	0.045 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179L, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179M	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.035	51	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179M, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179M	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.5	0.0066	380	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179M, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179M	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0022	0.049	0.045 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179M, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179P	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.324	0.005	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179P, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179P	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0016	0.098	0.02	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179P, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179Q	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.027	70	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179Q, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.3	0.0048	480	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179Q	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0047	0.034	0.14 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179Q, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179S	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.019	89	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.3	0.0074	310	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179S	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0015	0.028	0.054 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179S, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179T	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.9	0.0069	280		PMID 2182628	Open record: thymidylate synthase variant R179T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179T	Lactobacillus casei	5,10-methylenetetrahydrofolate	—	0.02	90		PMID 2182628	Open record: thymidylate synthase variant R179T, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant R179T	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0016	0.021	0.076 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179T, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179V	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.042	33	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179V, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179V	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.9	0.0063	300	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179V	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0013	0.035	0.037 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179V, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179W	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.058	48	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179W, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R179W	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	2.6	0.0055	470	25 °C	PMID 10964985	Open record: thymidylate synthase variant R179W, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R179W	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.0019	0.042	0.045 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R179W, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.005	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	1.4 × 10⁻⁴	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218H	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	4.5 × 10⁻⁴	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218H, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218K	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.096	1.9	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R218K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.18	0.3	6	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.001	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.0021	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	7.1 × 10⁻⁴	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218V	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	8.5 × 10⁻⁴	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R218Y	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	7.4 × 10⁻⁴	—	—	25 °C	PMID 10964985	Open record: thymidylate synthase variant R218Y, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23A	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.558	0.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.21	0.052	4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23A	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.0053	1.3 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23A, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23D	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.31	0.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23D, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23D	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.12	0.009	13	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23D, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23D	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.012	0.58 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23D, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23E	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.152	1.6	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23E, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.25	0.051	4.9	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23E	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.016	0.44 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23E, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23F	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.444	0.3	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23F, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23F	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.12	0.029	4.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23F, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23G	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.473	0.3	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23G, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23G	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.12	0.027	4.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23G	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.009	0.018	0.50 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23G, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23I	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.038	0.18 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23I, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23K	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.358	0.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.16	0.02	8	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23K	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.011	0.011	1.0 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23K, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23L	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.786	0.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23L, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.11	0.027	4.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23L	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.006	0.0047	1.3 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23L, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23N	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.387	0.2	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23N, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23N	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.06	0.02	3	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23N, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23N	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.01	0.011	0.91 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23N, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23P	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.79	0.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23P, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23P	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.04	0.035	1.1	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23P, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23Q	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.221	2.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23Q, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.53	0.022	24	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23S	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.284	0.7	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.2	0.019	11	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23S	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.01	0.016	0.62 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23S, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23V	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.422	0.6	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23V, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23V	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.27	0.032	8.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23V, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23V	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.024	0.29 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23V, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23Y	Lactobacillus casei	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.423	0.4	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23Y, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthasevariant R23Y	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	0.15	0.005	30	25 °C	PMID 10964985	Open record: thymidylate synthase variant R23Y, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant R23Y	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.01	0.019	0.53 calc.	pH 7.4, 25 °C	PMID 10964985	Open record: thymidylate synthase variant R23Y, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316A	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0012	690	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316A, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316A	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.81	0.37	2.2	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316A, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316A	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.015	0.024	0.62 calc.		PMID 1627545	Open record: thymidylate synthase variant V316A, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316C	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0016	720	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316C, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316C	Lactobacillus casei	5,10-methylenetetrahydrofolate	1.1	0.077	15	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316C, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316E	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0025	60	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316E, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316E	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.15	0.83	0.18	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316E, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316E	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.01	0.022	0.45 calc.		PMID 1627545	Open record: thymidylate synthase variant V316E, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316F	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0022	590	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316F, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316F	Lactobacillus casei	5,10-methylenetetrahydrofolate	1.3	0.065	20	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316F, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316G	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0056	5.4	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316G, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316G	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.03	0.38	0.078	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316G, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316G	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.008	0.01	0.80 calc.		PMID 1627545	Open record: thymidylate synthase variant V316G, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316H	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0016	340	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316H, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316H	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.55	0.05	11	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316H, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316I	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0022	1.7 × 10³	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316I, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316I	Lactobacillus casei	5,10-methylenetetrahydrofolate	3.8	0.035	110	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316I, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316K	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0012	99	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316K, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316K	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.12	0.085	1.4	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316K, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316K	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.013	0.016	0.81 calc.		PMID 1627545	Open record: thymidylate synthase variant V316K, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316L	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0017	810	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316L, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316L	Lactobacillus casei	5,10-methylenetetrahydrofolate	1.3	0.084	16	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316L, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316M	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0025	260	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316M, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316M	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.65	0.12	5.4	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316M, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316N	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0014	270	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316N, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316N	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.39	0.17	2.3	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316N, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316P	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0042	140	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316P, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316P	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.6	0.57	1.1	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316P, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316Q	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0031	100	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316Q, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316Q	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.32	0.28	1.1	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316Q, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316Q	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.013	0.015	0.87 calc.		PMID 1627545	Open record: thymidylate synthase variant V316Q, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316R	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0015	14	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316R, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316R	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.02	0.13	0.15	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316R, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316S	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0017	320	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316S, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316S	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.54	0.18	3	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316S, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316S	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.008	0.012	0.67 calc.		PMID 1627545	Open record: thymidylate synthase variant V316S, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316T	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0035	340	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316T, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316T	Lactobacillus casei	5,10-methylenetetrahydrofolate	1.2	0.14	8.1	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316T, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316T	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.01	0.015	0.67 calc.		PMID 1627545	Open record: thymidylate synthase variant V316T, 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316W	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0015	34	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316W, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316W	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.05	0.3	0.17	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316W, 5,10-methylenetetrahydrofolate
thymidylate synthasevariant V316Y	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0024	120	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316Y, 2'-deoxyuridine 5'-monophosphate
thymidylate synthasevariant V316Y	Lactobacillus casei	5,10-methylenetetrahydrofolate	0.29	0.17	1.7	25 °C	PMID 1627545	Open record: thymidylate synthase variant V316Y, 5,10-methylenetetrahydrofolate
thymidylate synthase (TS)	Lactobacillus casei	2'-deoxyuridine-5'-monophosphate	8	0.007	1.1 × 10³	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS), 2'-deoxyuridine-5'-monophosphate
thymidylate synthase (TS)	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.018	—	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS), 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant H199A/N229D	Lactobacillus casei	2'-deoxycytidine 5'-monophosphate	1.76	0.006	290	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199A/N229D, 2'-deoxycytidine 5'-monophosphate
thymidylate synthase (TS)variant H199A/N229D	Lactobacillus casei	2'-deoxyuridine-5'-monophosphate	0.0019	0.28	0.0068	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199A/N229D, 2'-deoxyuridine-5'-monophosphate
thymidylate synthase (TS)variant H199A/N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.029	60	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199A/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant H199G/N229D	Lactobacillus casei	2'-deoxycytidine 5'-monophosphate	2.54	0.012	220	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199G/N229D, 2'-deoxycytidine 5'-monophosphate
thymidylate synthase (TS)variant H199G/N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.031	80	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199G/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant H199N/N229D	Lactobacillus casei	2'-deoxycytidine 5'-monophosphate	0.33	0.008	40	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199N/N229D, 2'-deoxycytidine 5'-monophosphate
thymidylate synthase (TS)variant H199N/N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.097	3	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199N/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant H199P/N229D	Lactobacillus casei	2'-deoxyuridine-5'-monophosphate	0.015	—	—	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199P/N229D, 2'-deoxyuridine-5'-monophosphate
thymidylate synthase (TS)variant H199V/N229D	Lactobacillus casei	2'-deoxycytidine 5'-monophosphate	1.93	0.013	150	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199V/N229D, 2'-deoxycytidine 5'-monophosphate
thymidylate synthase (TS)variant H199V/N229D	Lactobacillus casei	2'-deoxyuridine-5'-monophosphate	7 × 10⁻⁴	0.47	0.0015	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199V/N229D, 2'-deoxyuridine-5'-monophosphate
thymidylate synthase (TS)variant H199V/N229D	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	—	0.013	150	pH 7.4	PMID 9398324	Open record: thymidylate synthase (TS) variant H199V/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant Δ90-139 (TSDel1)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.011	0.0051	2.2 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139 (TSDel1), 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5)	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0089	—	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5)	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	0.146	0.772	0.189 calc.	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.004	0.017	0.24 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T (TSDel3)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.0114	0.61 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T (TSDel3), 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4)	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.045	—	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4)	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	0.011	3.09	0.0036 calc.	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.016	0.0041	3.9 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/K89N (TSDel2)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.007	0.018	0.39 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/K89N (TSDel2), 5-bromo-2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6)	Lactobacillus casei	2'-deoxyuridine 5'-monophosphate	—	0.0181	—	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 2'-deoxyuridine 5'-monophosphate
thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6)	Lactobacillus casei	5,10-methylene-5,6,7,8-tetrahydrofolate	0.2	0.481	0.42 calc.	pH 7.4, 20 °C	PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 5,10-methylene-5,6,7,8-tetrahydrofolate
thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6)	Lactobacillus casei	5-bromo-2'-deoxyuridine 5'-monophosphate	0.004	0.0171	0.23 calc.		PMID 8180187	Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 5-bromo-2'-deoxyuridine 5'-monophosphate