Values CatLog read from this paper
Partial restoration of activity to Lactobacillus casei thymidylate synthase following inactivation by domain deletion
PMID 8180187doi:10.1021/bi00184a034Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (21 values) from this paper: thymidylate synthase (TS) from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase (TS)variant Δ90-139 (TSDel1) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.011 | 0.0051 | 2.2 calc. | Open record: thymidylate synthase (TS) variant Δ90-139 (TSDel1), 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5) | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0089 | — | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5) | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | 0.146 | 0.772 | 0.189 calc. | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant Δ90-139/F87D (TSDel5) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.004 | 0.017 | 0.24 calc. | Open record: thymidylate synthase (TS) variant Δ90-139/F87D (TSDel5), 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T (TSDel3) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.0114 | 0.61 calc. | Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T (TSDel3), 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4) | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.045 | — | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4) | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | 0.011 | 3.09 | 0.0036 calc. | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.016 | 0.0041 | 3.9 calc. | Open record: thymidylate synthase (TS) variant Δ90-139/H66Q/R72A/L75H/N79V/H80T/F87D (TSDel4), 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthase (TS)variant Δ90-139/K89N (TSDel2) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.018 | 0.39 calc. | Open record: thymidylate synthase (TS) variant Δ90-139/K89N (TSDel2), 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6) | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0181 | — | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6) | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | 0.2 | 0.481 | 0.42 calc. | pH 7.4, 20 °C | Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6) | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.004 | 0.0171 | 0.23 calc. | Open record: thymidylate synthase (TS) variant Δ90-139/R72A/L75H/H80T/F87D (TSDel6), 5-bromo-2'-deoxyuridine 5'-monophosphate |