Values CatLog read from this paper
Isolation and characterization of a mutant dihydrofolate reductase-thymidylate synthase from methotrexate-resistant Leishmania cells
PMID 8144647doi:10.1016/s0021-9258(17)34100-5Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (12 values) from this paper: dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity, dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity from Leishmania major.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity | Leishmania major | dihydrofolate | 29 | 0.0013 | 2.2 × 10⁴ calc. | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity, dihydrofolate |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity | Leishmania major | NADPH | — | 9 × 10⁻⁴ | — | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity, NADPH |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityvariant M53R | Leishmania major | dihydrofolate | 7.6 | 0.0016 | 4.8 × 10³ calc. | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity variant M53R, dihydrofolate |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityvariant M53R | Leishmania major | NADPH | — | 0.0012 | — | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activity variant M53R, NADPH |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity | Leishmania major | (6R,S)-5,10-methylenetetrahydrofolate | — | 0.079 | — | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity, (6R,S)-5,10-methylenetetrahydrofolate |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity | Leishmania major | 2'-deoxyuridine 5'-monophosphate | 5.8 | 0.007 | 830 calc. | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity, 2'-deoxyuridine 5'-monophosphate |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activityvariant M53R | Leishmania major | (6R,S)-5,10-methylenetetrahydrofolate | — | 0.096 | — | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity variant M53R, (6R,S)-5,10-methylenetetrahydrofolate |
| dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activityvariant M53R | Leishmania major | 2'-deoxyuridine 5'-monophosphate | 5.5 | 0.0065 | 850 calc. | 25 °C | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), TS activity variant M53R, 2'-deoxyuridine 5'-monophosphate |