CatLogenzyme kinetics catalog

EC 1.5.1.3: dihydrofolate reductase

OxidoreductasesOpen in Browse

CatLog read 380 records for EC 1.5.1.3 from 27 papers, for 15 organisms. Names in these papers: dihydrofolate reductase, dihydrofolate reductase (DHFR), dihydrofolate reductase (hDHFR), R67 dihydrofolate reductase and 18 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 368 records for EC 1.5.1.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.5.1.3 in Browse.

Papers

PaperOrganismsRecords
Probing the interactions between the folding elements early in the folding of Escherichia coli dihydrofolate reductase by systematic sequence perturbation analysisEscherichia coli52
Kinetic and structural analysis for potent antifolate inhibition of Pneumocystis jirovecii, Pneumocystis carinii, and human dihydrofolate reductases and their active-site variantsHomo sapiens, Pneumocystis carinii and 1 more51
Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductaseEscherichia coli22
Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductaseMycobacterium avium20
Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexesHomo sapiens, Pneumocystis carinii and 1 more20
Probing the roles of conserved arginine-44 of Escherichia coli dihydrofolate reductase in its function and stability by systematic sequence perturbation analysisEscherichia coli20
Conflicting requirements of Plasmodium falciparum dihydrofolate reductase mutations conferring resistance to pyrimethamine-WR99210 combinationPlasmodium falciparum16
Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markersHomo sapiens16
Breaking symmetry: mutations engineered into R67 dihydrofolate reductase, a D2 symmetric homotetramer possessing a single active site pore—14
"Catch 222," the effects of symmetry on ligand binding and catalysis in R67 dihydrofolate reductase as determined by mutations at Tyr-69—14
Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactionsEscherichia coli14
Role of S65, Q67, I68, and Y69 residues in homotetrameric R67 dihydrofolate reductase—12
Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductaseHomo sapiens11
Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutantsEscherichia coli11
Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations—10
Cloning and characterization of dihydrofolate reductases from deep-sea bacteriaEscherichia coli, Moritella japonica and 3 more10
Atypical transient state kinetics of recombinant human dihydrofolate reductase produced by hysteretic behavior. Comparison with dihydrofolate reductases from other sourcesEscherichia coli, Gallus gallus and 2 more9
Pyrimethamine-resistant dihydrofolate reductase enzymes of Plasmodium falciparum are not enzymatically compromised in vitroPlasmodium falciparum8
A mechanism of resistance to methotrexate. NADPH but not NADH stimulation of methotrexate binding to dihydrofolate reductaseMus musculus8
Role of the active-site carboxylate in dihydrofolate reductase: kinetic and spectroscopic studies of the aspartate 26-->asparagine mutant of the Lactobacillus casei enzymeLactobacillus casei8
Plasmodium falciparum: asparagine mutant at residue 108 of dihydrofolate reductase is an optimal antifolate-resistant single mutantPlasmodium falciparum8
Chimeric dihydrofolate reductases display properties of modularity and biophysical diversityEscherichia coli7
Role of lysine-54 in determining cofactor specificity and binding in human dihydrofolate reductaseHomo sapiens6
Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in…Escherichia coli4
Enzymatic and Mutational Analysis of the PruA Pteridine Reductase Required for Pterin-Dependent Control of Biofilm Formation in Agrobacterium tumefaciensPseudomonas aeruginosa4
Isolation and characterization of a mutant dihydrofolate reductase-thymidylate synthase from methotrexate-resistant Leishmania cellsLeishmania major4
The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania majorLeishmania major1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
dihydrofolate reductaseEscherichia coli7,8-dihydrofolate4.5——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseEscherichia coli7,8-dihydrofolate24.80.00131.92 × 10⁴pH 7, 25 °CPMID 20045086Open
dihydrofolate reductaseEscherichia colidihydrofolateTable 15.30.00124.4 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductaseEscherichia colidihydrofolateenzyme incubated 24 h at 4 °C in 0.1 M H2O2 before the assay3.10.00142.2 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductaseEscherichia colidihydrofolatein the text——4.4 × 10³pH 7, 15 °CPMID 16510443Open
dihydrofolate reductaseEscherichia colidihydrofolate5.40.00163.4 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant C85A/C152S (AS-DHFR)Escherichia colidihydrofolate4.90.00124.1 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M16IEscherichia colidihydrofolate17——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M16KEscherichia colidihydrofolate18——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M16LEscherichia colidihydrofolate7——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M16NEscherichia colidihydrofolate16.1——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/C85A/C152S (M1MA)Escherichia colidihydrofolate4.60.00133.5 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF)Escherichia colidihydrofolateTable 125.80.0038.6 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF)Escherichia colidihydrofolatein the text——8.6 × 10³pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF)Escherichia colidihydrofolate40.50.00834.9 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF)Escherichia colidihydrofolateenzyme incubated 24 h at 4 °C in 0.1 M H2O2 before the assay37.90.00894.3 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1A/M16S/M20L/M42Y/M92F/C85A/C152S (ASLYF)Escherichia colidihydrofolate16.20.00384.3 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1P/C85A/C152S (M1MP)Escherichia colidihydrofolate4.80.00124.0 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M1S/C85A/C152S (M1MS)Escherichia colidihydrofolate5.30.00252.1 × 10³ calc.pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M42AEscherichia colidihydrofolate140.00131.08 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42CEscherichia colidihydrofolate30.40.001941.57 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42EEscherichia colidihydrofolate15.90.005972.7 × 10³pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42GEscherichia colidihydrofolate130.001181.1 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42HEscherichia colidihydrofolate26.60.001931.38 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42IEscherichia colidihydrofolate12.46.1 × 10⁻⁴2.03 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42IEscherichia colidihydrofolate6.2——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M42LEscherichia colidihydrofolate18.90.001121.69 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42LEscherichia colidihydrofolate11.8——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M42PEscherichia colidihydrofolate248.3 × 10⁻⁴2.89 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42QEscherichia colidihydrofolate24.30.002271.07 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42SEscherichia colidihydrofolate22.60.002271 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42TEscherichia colidihydrofolate18.78.2 × 10⁻⁴2.28 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42VEscherichia colidihydrofolate15.79.1 × 10⁻⁴1.73 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42WEscherichia colidihydrofolate104.60.0452.3 × 10³pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42YEscherichia colidihydrofolate45.50.001782.56 × 10⁴pH 7, 25 °CPMID 15944418Open
dihydrofolate reductasevariant M42YEscherichia colidihydrofolate20.2——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M92FEscherichia colidihydrofolate6.8——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M92IEscherichia colidihydrofolate7.9——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant M92LEscherichia colidihydrofolate6.3——pH 7, 15 °CPMID 16510443Open
dihydrofolate reductasevariant R44AEscherichia colidihydrofolate3.050.00161.9 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44CEscherichia colidihydrofolate2.430.00112.2 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44DEscherichia colidihydrofolate0.690.00323015 °CPMID 20043879Open
dihydrofolate reductasevariant R44EEscherichia colidihydrofolate0.890.001947015 °CPMID 20043879Open
dihydrofolate reductasevariant R44FEscherichia colidihydrofolate1.390.002750015 °CPMID 20043879Open
dihydrofolate reductasevariant R44GEscherichia colidihydrofolate1.780.00151.2 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44HEscherichia colidihydrofolate1.597 × 10⁻⁴2.5 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44IEscherichia colidihydrofolate1.150.002156015 °CPMID 20043879Open
dihydrofolate reductasevariant R44KEscherichia colidihydrofolate5.70.00252.3 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44LEscherichia colidihydrofolate0.980.001468015 °CPMID 20043879Open
dihydrofolate reductasevariant R44MEscherichia colidihydrofolate3.80.00231.6 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44NEscherichia colidihydrofolate3.30.00251.3 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44PEscherichia colidihydrofolate0.30.002910015 °CPMID 20043879Open
dihydrofolate reductasevariant R44QEscherichia colidihydrofolate1.90.002480015 °CPMID 20043879Open
dihydrofolate reductasevariant R44SEscherichia colidihydrofolate5.80.00311.9 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44TEscherichia colidihydrofolate20.002580015 °CPMID 20043879Open
dihydrofolate reductasevariant R44VEscherichia colidihydrofolate1.318 × 10⁻⁴1.7 × 10³15 °CPMID 20043879Open
dihydrofolate reductasevariant R44WEscherichia colidihydrofolate2.410.003766015 °CPMID 20043879Open
dihydrofolate reductasevariant R44YEscherichia colidihydrofolate0.890.00190015 °CPMID 20043879Open
dihydrofolate reductasevariant ΔA145 (Δ145)Escherichia coli7,8-dihydrofolate248 × 10⁻⁴2.94 × 10⁴pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔG121 (Δ121)Escherichia coli7,8-dihydrofolate1.70.0108160pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔG121/ΔA145 (Δ121/Δ145)Escherichia coli7,8-dihydrofolate0.220.007330pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔG67 (Δ67)Escherichia coli7,8-dihydrofolate16.50.00513.3 × 10³pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔG67/ΔA145 (Δ67/Δ145)Escherichia coli7,8-dihydrofolate41.10.00488.6 × 10³pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔG67/ΔG121 (Δ67/Δ121)Escherichia coli7,8-dihydrofolate0.380.005670pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔR52 (Δ52)Escherichia coli7,8-dihydrofolate96.30.1161830pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔR52/ΔA145 (Δ52/Δ145)Escherichia coli7,8-dihydrofolate31.90.0777410pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔR52/ΔG121 (Δ52/Δ121)Escherichia coli7,8-dihydrofolate0.370.051210pH 7, 25 °CPMID 20045086Open
dihydrofolate reductasevariant ΔR52/ΔG67 (Δ52/Δ67)Escherichia coli7,8-dihydrofolate23.10.0502460pH 7, 25 °CPMID 20045086Open
dihydrofolate reductaseGallus gallus7,8-dihydrofolate2.6——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 µM dihydrofolate and 100 µM NADPH; Table I7.9——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; Table II11.6——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat9.1——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat11.7——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat8——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductasevariant F31LHomo sapiens7,8-dihydrofolate15.1——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseLactobacillus casei7,8-dihydrofolate250.0012.5 × 10⁴pH 5.5, 25 °CPMID 7893701Open
dihydrofolate reductaseLactobacillus casei7,8-dihydrofolate100.0011 × 10⁴pH 7.5, 25 °CPMID 7893701Open
dihydrofolate reductaseLactobacillus caseifolic acid—6 × 10⁻⁴—pH 5.5, 25 °CPMID 7893701Open
dihydrofolate reductaseLactobacillus caseifolic acid0.060.0292pH 7.5, 25 °CPMID 7893701Open
dihydrofolate reductasevariant D26NLactobacillus casei7,8-dihydrofolate5.50.00163.4 × 10³pH 5.5, 25 °CPMID 7893701Open
dihydrofolate reductasevariant D26NLactobacillus casei7,8-dihydrofolate1.10.0014790pH 7.5, 25 °CPMID 7893701Open
dihydrofolate reductasevariant D26NLactobacillus caseifolic acid—6 × 10⁻⁴—pH 5.5, 25 °CPMID 7893701Open
dihydrofolate reductasevariant D26NLactobacillus caseifolic acid0.00240.0160.15pH 7.5, 25 °CPMID 7893701Open
dihydrofolate reductaseMus musculus7,8-dihydrofolate5.7——pH 7.65, 20 °CPMID 2492521Open
dihydrofolate reductaseMus musculusdihydrofolateno added salt; cosubstrate NADPH5——pH 7, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateplus 0.15 M KCl; cosubstrate NADPH12.5——pH 7, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateno added salt; cosubstrate NADH5——pH 7, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateplus 0.15 M KCl; cosubstrate NADH0.52——pH 7, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateno added salt; cosubstrate NADPH6.7——pH 5, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateplus 0.15 M KCl; cosubstrate NADPH17——pH 5, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateno added salt; cosubstrate NADH2.5——pH 5, 37 °CPMID 6882460Open
dihydrofolate reductaseMus musculusdihydrofolateplus 0.15 M KCl; cosubstrate NADH1.03——pH 5, 37 °CPMID 6882460Open
dihydrofolate reductasevariant S108APlasmodium falciparum7,8-dihydrofolate210.0141.5 × 10³25 °CPMID 9371090Open
dihydrofolate reductasevariant S108APlasmodium falciparumreduced nicotinamide adenine dinucleotide phosphate—0.009—25 °CPMID 9371090Open
dihydrofolate reductasevariant S108CPlasmodium falciparum7,8-dihydrofolate120.02350025 °CPMID 9371090Open
dihydrofolate reductasevariant S108CPlasmodium falciparumreduced nicotinamide adenine dinucleotide phosphate—0.009—25 °CPMID 9371090Open
dihydrofolate reductasevariant S108GPlasmodium falciparum7,8-dihydrofolate230.021.1 × 10³25 °CPMID 9371090Open
dihydrofolate reductasevariant S108GPlasmodium falciparumreduced nicotinamide adenine dinucleotide phosphate—0.009—25 °CPMID 9371090Open
dihydrofolate reductasevariant S108QPlasmodium falciparum7,8-dihydrofolate370.0570025 °CPMID 9371090Open
dihydrofolate reductasevariant S108QPlasmodium falciparumreduced nicotinamide adenine dinucleotide phosphate—0.014—25 °CPMID 9371090Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)Plasmodium falciparum7,8-dihydrofolate1.240.044428pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)Plasmodium falciparumNADPH—0.0232—pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108NPlasmodium falciparum7,8-dihydrofolate1.230.020959pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108NPlasmodium falciparumNADPH—0.0379—pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/164LPlasmodium falciparum7,8-dihydrofolate1.420.020769pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/164LPlasmodium falciparumNADPH—0.0373—pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/213APlasmodium falciparum7,8-dihydrofolate0.680.010266pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR domain of the bifunctional DHFR-thymidylate synthase)variant 51I/59R/108N/213APlasmodium falciparumNADPH—0.0309—pH 7, 25 °CPMID 17451822Open
dihydrofolate reductase (DHFR)Escherichia coli7,8-dihydrofolate138 × 10⁻⁴1.6 × 10⁴pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)Escherichia colidihydrofolate6.188.9 × 10⁻⁴6.92 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)chiHi (chimera: helix residues 25-34 replaced by the Haemophilus influenza segment PADLAWFRQK)Escherichia coli7,8-dihydrofolate104 × 10⁻⁴2.5 × 10⁴pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)chiHs (chimera: helix residues 25-34 replaced by the Homo sapiens segment RNEFRYFQRM)Escherichia coli7,8-dihydrofolate90.067130pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)chiLg (chimera: helix residues 25-34 replaced by the Listeria grayi segment PADLAYFKKQ)Escherichia coli7,8-dihydrofolate147 × 10⁻⁴2.2 × 10⁴pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)chiPf (chimera: helix residues 25-34 replaced by the Plasmodium falciparum segment SLDMKYFCAV)Escherichia coli7,8-dihydrofolate330.058500pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)chiSc (chimera: helix residues 25-34 replaced by the Saccharomyces cerevisiae segment PSEMKYFRQV)Escherichia coli7,8-dihydrofolate290.00152.4 × 10⁴pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)chiSd (chimera: helix residues 25-34 replaced by the Streptococcus dysgalactiae segment PRELQHFKET)Escherichia coli7,8-dihydrofolate370.00636 × 10³pH 7, 25 °CPMID 31095809Open
dihydrofolate reductase (DHFR)variant I155AEscherichia colidihydrofolate5.20.001443.62 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155DEscherichia colidihydrofolate7.550.00223.43 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155EEscherichia colidihydrofolate5.80.001763.29 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155KEscherichia colidihydrofolate4.677.5 × 10⁻⁴6.22 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155LEscherichia colidihydrofolate4.980.001692.95 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155QEscherichia colidihydrofolate7.380.001564.73 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155REscherichia colidihydrofolate8.070.002393.37 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155SEscherichia colidihydrofolate5.979.6 × 10⁻⁴6.24 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155TEscherichia colidihydrofolate4.920.001273.86 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155VEscherichia colidihydrofolate4.80.00153.19 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155WEscherichia colidihydrofolate6.750.002642.55 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant I155YEscherichia colidihydrofolate6.030.001514 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59CEscherichia colidihydrofolate4.970.001084.59 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59EEscherichia colidihydrofolate5.570.003451.62 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59GEscherichia colidihydrofolate3.920.001852.12 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59HEscherichia colidihydrofolate1.450.00111.31 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59IEscherichia colidihydrofolate4.180.003091.36 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59KEscherichia colidihydrofolate4.30.0136320pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59LEscherichia colidihydrofolate3.450.001821.9 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59MEscherichia colidihydrofolate3.650.002241.63 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59PEscherichia colidihydrofolate5.680.002432.34 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59QEscherichia colidihydrofolate4.930.002022.45 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59SEscherichia colidihydrofolate4.770.002621.82 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59TEscherichia colidihydrofolate10.30.004912.1 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59VEscherichia colidihydrofolate6.730.001823.69 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant N59WEscherichia colidihydrofolate5.020.00942530pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148AEscherichia colidihydrofolate4.85 × 10⁻⁴9.61 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148EEscherichia colidihydrofolate6.670.001096.12 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148KEscherichia colidihydrofolate6.884.1 × 10⁻⁴1.669 × 10⁴pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148NEscherichia colidihydrofolate7.828.3 × 10⁻⁴9.46 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148PEscherichia colidihydrofolate5.727.1 × 10⁻⁴8.04 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148REscherichia colidihydrofolate4.550.00162.84 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148TEscherichia colidihydrofolate8.220.003232.54 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant S148VEscherichia colidihydrofolate5.856.6 × 10⁻⁴8.92 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40AEscherichia colidihydrofolate9.570.001775.42 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40FEscherichia colidihydrofolate13.530.004463.04 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40HEscherichia colidihydrofolate9.530.007551.26 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40IEscherichia colidihydrofolate3.80.00182.11 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40LEscherichia colidihydrofolate4.830.001752.77 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40MEscherichia colidihydrofolate3.70.002231.66 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40NEscherichia colidihydrofolate3.420.002081.64 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40REscherichia colidihydrofolate7.820.001634.78 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant V40SEscherichia colidihydrofolate5.50.002212.49 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30AEscherichia colidihydrofolate14.50.002146.79 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30EEscherichia colidihydrofolate11.580.001358.59 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30HEscherichia colidihydrofolate13.120.001946.77 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30MEscherichia colidihydrofolate11.90.002115.65 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30NEscherichia colidihydrofolate13.980.002485.63 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30REscherichia colidihydrofolate14.080.00275.21 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30SEscherichia colidihydrofolate150.002097.17 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)variant W30YEscherichia colidihydrofolate13.470.002555.28 × 10³pH 7, 15 °CPMID 15740745Open
dihydrofolate reductase (DHFR)Mycobacterium aviumdihydrofolate20.57 × 10⁻⁴2.9 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)Mycobacterium aviumNADPH26.80.001551.73 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31AMycobacterium aviumdihydrofolate0.860.03723pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31AMycobacterium aviumNADPH0.596.8 × 10⁻⁴380pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31EMycobacterium aviumdihydrofolate3.580.001921.87 × 10³pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31EMycobacterium aviumNADPH2.050.002011.02 × 10³pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31LMycobacterium aviumdihydrofolate0.0250.1980.13pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31LMycobacterium aviumNADPH0.020.0014413.9pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31NMycobacterium aviumdihydrofolate5.030.002082.4 × 10³pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31NMycobacterium aviumNADPH8.57.7 × 10⁻⁴1.1 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31QMycobacterium aviumdihydrofolate1.50.00232650pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant D31QMycobacterium aviumNADPH2.186.5 × 10⁻⁴3.35 × 10³pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32AMycobacterium aviumdihydrofolate16.29.6 × 10⁻⁴1.69 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32AMycobacterium aviumNADPH17.80.001391.28 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32DMycobacterium aviumdihydrofolate7.20.005121.41 × 10³pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32DMycobacterium aviumNADPH108.4 × 10⁻⁴1.19 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32FMycobacterium aviumdihydrofolate216.8 × 10⁻⁴3.09 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant L32FMycobacterium aviumNADPH23.20.001321.76 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant V76AMycobacterium aviumdihydrofolate18.77.8 × 10⁻⁴2.4 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR)variant V76AMycobacterium aviumNADPH23.70.001561.52 × 10⁴pH 7, 30 °CPMID 17542991Open
dihydrofolate reductase (DHFR, EcoDHFR)Escherichia coli10-formyl-7,8-dihydrofolate4.580.0031.53 × 10³pH 7.5PMID 29799386Open
dihydrofolate reductase (DHFR, EcoDHFR)Escherichia coli7,8-dihydrofolate22.830.00554.15 × 10³pH 7.5PMID 29799386Open
dihydrofolate reductase (DHFR, EcoDHFR)Escherichia coliNADPHDHF 50 µM fixed; cosubstrate DHF28.070.0083.5 × 10³pH 7.5PMID 29799386Open
dihydrofolate reductase (DHFR, EcoDHFR)Escherichia coliNADPH10-CHO-DHF 50 µM fixed; cosubstrate 10-CHO-DHF4.050.0074547pH 7.5PMID 29799386Open
dihydrofolate reductase (hDHFR)Homo sapiens5,6-dihydrofolate10——pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)Homo sapiens7,8-dihydrofolic acid—3.2 × 10⁻⁵—pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)Homo sapiensdihydrofolic acid400.00271.5 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)Homo sapiensNADPH—0.004—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)Homo sapiensnicotinamide adenine dinucleotide (reduced)1.20.01867pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)Homo sapiensnicotinamide adenine dinucleotide phosphate (reduced)7.42.6 × 10⁻⁴2.85 × 10⁴pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)variant F31A/F34V/Q35HHomo sapiens5,6-dihydrofolate1.10.0043300pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31G/Q35NHomo sapiens5,6-dihydrofolate4.30.00172.6 × 10³pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31PHomo sapiens5,6-dihydrofolate6.40.00154.3 × 10³pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31P/Q35EHomo sapiens5,6-dihydrofolate1.70.0027600pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31P/Q35HHomo sapiens5,6-dihydrofolate1.70.0021800pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31R/F34A/Q35NHomo sapiens5,6-dihydrofolate0.50.0022200pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31R/F34T/Q35RHomo sapiens5,6-dihydrofolate10.002500pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31R/F34T/Q35SHomo sapiens5,6-dihydrofolate10.0017600pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31R/Q35EHomo sapiens5,6-dihydrofolate1.36.9 × 10⁻⁴1.9 × 10³pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant F31S/Q35EHomo sapiens5,6-dihydrofolate9.40.00332.8 × 10³pH 7.6, 23 °CPMID 17868689Open
dihydrofolate reductase (hDHFR)variant K54QHomo sapiens7,8-dihydrofolic acid—1.8 × 10⁻⁴—pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)variant K54QHomo sapiensnicotinamide adenine dinucleotide (reduced)20.07128pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)variant K54QHomo sapiensnicotinamide adenine dinucleotide phosphate (reduced)18.50.0151.2 × 10³pH 7.5PMID 2124504Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I23.8——pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I4.8——pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I—0.00386—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I—3.3 × 10⁻⁴—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I4.1——pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolatein the text——1.5 × 10⁴pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22FHomo sapiensNADPH—0.00234—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22RHomo sapiens7,8-dihydrofolateTable I0.0450.001628 calc.pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22RHomo sapiens7,8-dihydrofolatein the text——30pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22RHomo sapiensNADPH—0.0012—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22WHomo sapiens7,8-dihydrofolateTable I4.194.2 × 10⁻⁴1.0 × 10⁴ calc.pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22WHomo sapiens7,8-dihydrofolatein the text——1 × 10⁴pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22WHomo sapiensNADPH—0.00135—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22YHomo sapiens7,8-dihydrofolateTable I6.125.3 × 10⁻⁴1.2 × 10⁴ calc.pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22YHomo sapiens7,8-dihydrofolatein the text——1.2 × 10⁴pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant L22YHomo sapiensNADPH—9.1 × 10⁻⁴—pH 7.65, 20 °CPMID 7890613Open
dihydrofolate reductase (hDHFR)variant N64FHomo sapiensdihydrofolic acid408.1 × 10⁻⁴4.9 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant N64FHomo sapiensNADPH—0.0052—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant N64SHomo sapiensdihydrofolic acid471.8 × 10⁻⁴2.61 × 10⁵pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant N64SHomo sapiensNADPH—0.0063—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensdihydrofolic acid487.6 × 10⁻⁴6.3 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensNADPH—0.013—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensdihydrofolic acid434.9 × 10⁻⁴8.8 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensNADPH—0.0055—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensdihydrofolic acid350.00113.3 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensNADPH—0.0044—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensdihydrofolic acid270.00161.7 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensNADPH—0.0046—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pcDHFR)Pneumocystis cariniidihydrofolic acid2840.00495.8 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pcDHFR)Pneumocystis cariniiNADPH—0.0145—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniidihydrofolic acid360.00854 × 10³pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniiNADPH—0.0092—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciidihydrofolic acid530.00281.9 × 10⁴pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciiNADPH—0.0229—pH 7.4, 37 °CPMID 19196009Open
dihydrofolate reductase-thymidylate synthase (DHFR-TS)Leishmania majorfolate—0.0041—pH 5PMID 9153248Open
dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityLeishmania majordihydrofolate290.00132.2 × 10⁴ calc.25 °CPMID 8144647Open
dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityLeishmania majorNADPH—9 × 10⁻⁴—25 °CPMID 8144647Open
dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityvariant M53RLeishmania majordihydrofolate7.60.00164.8 × 10³ calc.25 °CPMID 8144647Open
dihydrofolate reductase-thymidylate synthase (DHFR-TS), DHFR activityvariant M53RLeishmania majorNADPH—0.0012—25 °CPMID 8144647Open
ecDHFR (dihydrofolate reductase)Escherichia colidihydrofolate18.40.00111.67 × 10⁴pH 7, 25 °CPMID 20040594Open
ecDHFR (dihydrofolate reductase)Escherichia coliNADPH19.20.00191.01 × 10⁴pH 7, 25 °CPMID 20040594Open
human dihydrofolate reductase (hDHFR)Homo sapiensdihydrofolic acid267 × 10⁻⁴3.7 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)Homo sapiensNADPH—0.0045—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant N64FHomo sapiensdihydrofolic acid288 × 10⁻⁴3.6 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant N64FHomo sapiensNADPH—0.0075—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant N64SHomo sapiensdihydrofolic acid369 × 10⁻⁴4.1 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant N64SHomo sapiensNADPH—0.0063—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensdihydrofolic acid395 × 10⁻⁴7.4 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensNADPH—0.0125—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensdihydrofolic acid434 × 10⁻⁴1.06 × 10⁵pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensNADPH—0.0057—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35K/N64SHomo sapiensdihydrofolic acid367 × 10⁻⁴5.1 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35K/N64SHomo sapiensNADPH—0.0066—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensdihydrofolic acid247 × 10⁻⁴3.7 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensNADPH—0.0038—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35S/N64FHomo sapiensdihydrofolic acid217 × 10⁻⁴3.2 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35S/N64FHomo sapiensNADPH—0.0056—pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensdihydrofolic acid234 × 10⁻⁴5.4 × 10⁴pH 7.4, 37 °CPMID 23545530Open
human dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensNADPH—0.0053—pH 7.4, 37 °CPMID 23545530Open
mjDHFR (dihydrofolate reductase)Moritella japonicadihydrofolate144.50.238600pH 7, 25 °CPMID 20040594Open
mjDHFR (dihydrofolate reductase)Moritella japonicaNADPH109.20.05841.9 × 10³pH 7, 25 °CPMID 20040594Open
myDHFR (dihydrofolate reductase)Moritella yayanosiidihydrofolate83.80.00184.66 × 10⁴pH 7, 25 °CPMID 20040594Open
myDHFR (dihydrofolate reductase)Moritella yayanosiiNADPH80.30.1011800pH 7, 25 °CPMID 20040594Open
PA3437 (PruA homolog, previously defined as FolM)Pseudomonas aeruginosa7,8-D-dihydroneopterin0.70.097.8pH 6PMID 32482721Open
PA3437 (PruA homolog, previously defined as FolM)Pseudomonas aeruginosa7,8-L-dihydrobiopterin0.80.075711pH 6PMID 32482721Open
PA3437 (PruA homolog, previously defined as FolM)Pseudomonas aeruginosa7,8-L-dihydromonapterin1.90.1711pH 6PMID 32482721Open
PA3437 (PruA homolog, previously defined as FolM)Pseudomonas aeruginosadihydrofolate3.20.059354pH 6PMID 32482721Open
PfDHFRvariant I51N/C59R/N108S/I164L (WR resist 1)Plasmodium falciparumdihydrofolate0.20.008324 calc.PMID 17875995Open
PfDHFRvariant I51N/C59R/N108S/I164L (WR resist 1)Plasmodium falciparumNADPH—0.0267—PMID 17875995Open
PfDHFRvariant N51I/C59R/N108S/I164L (WR resist 2)Plasmodium falciparumdihydrofolate1.50.0052290 calc.PMID 17875995Open
PfDHFRvariant N51I/C59R/N108S/I164L (WR resist 2)Plasmodium falciparumNADPH—0.052—PMID 17875995Open
PfDHFRvariant N51I/C59R/N108T/I164L (WR resist 3)Plasmodium falciparumdihydrofolate1.90.005380 calc.PMID 17875995Open
PfDHFRvariant N51I/C59R/N108T/I164L (WR resist 3)Plasmodium falciparumNADPH—0.00595—PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L (PfDHFR-QM template)Plasmodium falciparumdihydrofolate44.5——PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/D187A (PYR resist 1)Plasmodium falciparumdihydrofolate13.60.01453936 calc.PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/D187A (PYR resist 1)Plasmodium falciparumNADPH—0.0065—PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/I150V/N182I/N201D (PYR resist 3)Plasmodium falciparumdihydrofolate11.40.01465778 calc.PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/I150V/N182I/N201D (PYR resist 3)Plasmodium falciparumNADPH—0.01126—PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/K96N (PYR resist 2)Plasmodium falciparumdihydrofolate28.30.00813.5 × 10³ calc.PMID 17875995Open
PfDHFRvariant N51I/C59R/S108N/I164L/K96N (PYR resist 2)Plasmodium falciparumNADPH—0.0072—PMID 17875995Open
PfDHFR-TSvariant I51N/C59R/N108S/L164I/D54N (WR resist A)Plasmodium falciparumdihydrofolate0.78——PMID 17875995Open
PfDHFR-TSvariant N51I/C59R/S108N/I164L (PfDHFR-TS-QM template)Plasmodium falciparumdihydrofolate720.013325.4 × 10³ calc.PMID 17875995Open
PfDHFR-TSvariant N51I/C59R/S108N/I164L (PfDHFR-TS-QM template)Plasmodium falciparumNADPH—0.00643—PMID 17875995Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)Pneumocystis cariniidihydrofolic acid2350.00356.7 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)Pneumocystis cariniiNADPH—0.023—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69NPneumocystis cariniiNADPH—0.0163—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69SPneumocystis cariniidihydrofolic acid2040.00712.9 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69SPneumocystis cariniiNADPH—0.0192—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniidihydrofolic acid380.00666 × 10³pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniiNADPH—0.0072—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69NPneumocystis cariniidihydrofolic acid2230.01072.1 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69NPneumocystis cariniiNADPH—0.0163—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69SPneumocystis cariniidihydrofolic acid1580.01859 × 10³pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69SPneumocystis cariniiNADPH—0.0106—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37SPneumocystis cariniidihydrofolic acid450.01014 × 10³pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37SPneumocystis cariniiNADPH—0.0066—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69NPneumocystis cariniidihydrofolic acid1900.00972 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69NPneumocystis cariniiNADPH—0.0167—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69SPneumocystis cariniidihydrofolic acid1570.01161.4 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69SPneumocystis cariniiNADPH—0.007—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciidihydrofolic acid360.0021.8 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciiNADPH—0.0189—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69FPneumocystis jiroveciidihydrofolic acid570.00321.8 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69FPneumocystis jiroveciiNADPH—0.0149—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69NPneumocystis jiroveciidihydrofolic acid780.00243.3 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69NPneumocystis jiroveciiNADPH—0.0864—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37QPneumocystis jiroveciidihydrofolic acid510.00351.5 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37QPneumocystis jiroveciiNADPH—0.0118—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69FPneumocystis jiroveciidihydrofolic acid260.0039 × 10³pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69FPneumocystis jiroveciiNADPH—0.0275—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69NPneumocystis jiroveciidihydrofolic acid950.00382.5 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69NPneumocystis jiroveciiNADPH—0.0173—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69FPneumocystis jiroveciidihydrofolic acid830.00481.7 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69FPneumocystis jiroveciiNADPH—0.0162—pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69NPneumocystis jiroveciidihydrofolic acid470.00341.4 × 10⁴pH 7.4, 37 °CPMID 23545530Open
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69NPneumocystis jiroveciiNADPH—0.0252—pH 7.4, 37 °CPMID 23545530Open
ppDHFR (dihydrofolate reductase)Photobacterium profundumdihydrofolate1560.00622.52 × 10⁴pH 7, 25 °CPMID 20040594Open
ppDHFR (dihydrofolate reductase)Photobacterium profundumNADPH150.50.01987.6 × 10³pH 7, 25 °CPMID 20040594Open
R67 dihydrofolate reductase—7,8-dihydrofolate0.850.0076110pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductase—nicotinamide adenine dinucleotide phosphate (reduced)—0.0039220pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1—7,8-dihydrofolate0.560.00693pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1—nicotinamide adenine dinucleotide phosphate (reduced)—0.0036160pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+2—7,8-dihydrofolate0.20.014414pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+2—nicotinamide adenine dinucleotide phosphate (reduced)—0.017112pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+3—7,8-dihydrofolate——9pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+3—nicotinamide adenine dinucleotide phosphate (reduced)——26pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+4—7,8-dihydrofolate0.10.010510pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant K32M:1+4—nicotinamide adenine dinucleotide phosphate (reduced)—0.005419pH 7, 30 °CPMID 15333636Open
R67 dihydrofolate reductasevariant S59A/H362L (Quad3)—dihydrofolate0.810.0067120 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/H362L (Quad3)—NADPH—0.0044—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/H362L (Q67H:1)—dihydrofolate0.230.003664 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/H362L (Q67H:1)—NADPH—0.0013—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/H362L/Q367H (Q67H:1+4)—dihydrofolate0.150.002658 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/H362L/Q367H (Q67H:1+4)—NADPH—9.5 × 10⁻⁴—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/H362L (Q67H:1+2)—dihydrofolate0.210.003658 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/H362L (Q67H:1+2)—NADPH—2.8 × 10⁻⁴—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3)—dihydrofolate0.090.007512 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3)—NADPH—4.5 × 10⁻⁴—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4)—dihydrofolate0.11.3 × 10⁻⁴770 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4)—NADPH—2.6 × 10⁻⁵—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q267H/H362L (Q67H:1+3)—dihydrofolate0.230.01121 calc.pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant S59A/Q67H/Q267H/H362L (Q67H:1+3)—NADPH—0.0018—pH 7, 30 °CPMID 12501195Open
R67 dihydrofolate reductasevariant Y69F:1—dihydrofolate0.980.02147 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1—reduced nicotinamide adenine dinucleotide phosphate—0.0064—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2—dihydrofolate0.960.0248 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2—reduced nicotinamide adenine dinucleotide phosphate—0.014—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2+3—dihydrofolate1.40.03540 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2+3—reduced nicotinamide adenine dinucleotide phosphate—0.021—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2+3+4—dihydrofolate1.40.05426 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+2+3+4—reduced nicotinamide adenine dinucleotide phosphate—0.04—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+3—dihydrofolate0.560.0228 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+3—reduced nicotinamide adenine dinucleotide phosphate—0.0031—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+4—dihydrofolate1.90.02868 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69F:1+4—reduced nicotinamide adenine dinucleotide phosphate—0.014—pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69L—dihydrofolate0.160.180.89 calc.pH 7PMID 15333637Open
R67 dihydrofolate reductasevariant Y69L—reduced nicotinamide adenine dinucleotide phosphate—0.068—pH 7PMID 15333637Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68L—dihydrofolate0.320.02413 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68L—reduced nicotinamide adenine dinucleotide phosphate—0.026—pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68M—dihydrofolate0.170.0256.8 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68M—reduced nicotinamide adenine dinucleotide phosphate—0.021—pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Q67C—dihydrofolate0.10.0551.8 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Q67C—reduced nicotinamide adenine dinucleotide phosphate—0.026—pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant S65A—dihydrofolate1.10.004280 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant S65A—reduced nicotinamide adenine dinucleotide phosphate—0.0029—pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69F—dihydrofolate2.50.04457 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69F—reduced nicotinamide adenine dinucleotide phosphate—0.066—pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69H—dihydrofolate0.0140.0460.30 calc.pH 7, 30 °CPMID 11560482Open
R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69H—reduced nicotinamide adenine dinucleotide phosphate—0.176—pH 7, 30 °CPMID 11560482Open
svDHFR (dihydrofolate reductase)Shewanella violaceadihydrofolate90.10.00194.74 × 10⁴pH 7, 25 °CPMID 20040594Open
svDHFR (dihydrofolate reductase)Shewanella violaceaNADPH86.80.00342.55 × 10⁴pH 7, 25 °CPMID 20040594Open