Values CatLog read from this paper
Cloning and characterization of dihydrofolate reductases from deep-sea bacteria
PMID 20040594doi:10.1093/jb/mvp206Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: ecDHFR (dihydrofolate reductase), mjDHFR (dihydrofolate reductase), myDHFR (dihydrofolate reductase) and 2 more from Escherichia coli, Moritella japonica, Moritella yayanosii and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ecDHFR (dihydrofolate reductase) | Escherichia coli | dihydrofolate | 18.4 | 0.0011 | 1.67 × 10⁴ | pH 7, 25 °C | Open record: ecDHFR (dihydrofolate reductase), dihydrofolate |
| ecDHFR (dihydrofolate reductase) | Escherichia coli | NADPH | 19.2 | 0.0019 | 1.01 × 10⁴ | pH 7, 25 °C | Open record: ecDHFR (dihydrofolate reductase), NADPH |
| mjDHFR (dihydrofolate reductase) | Moritella japonica | dihydrofolate | 144.5 | 0.238 | 600 | pH 7, 25 °C | Open record: mjDHFR (dihydrofolate reductase), dihydrofolate |
| mjDHFR (dihydrofolate reductase) | Moritella japonica | NADPH | 109.2 | 0.0584 | 1.9 × 10³ | pH 7, 25 °C | Open record: mjDHFR (dihydrofolate reductase), NADPH |
| myDHFR (dihydrofolate reductase) | Moritella yayanosii | dihydrofolate | 83.8 | 0.0018 | 4.66 × 10⁴ | pH 7, 25 °C | Open record: myDHFR (dihydrofolate reductase), dihydrofolate |
| myDHFR (dihydrofolate reductase) | Moritella yayanosii | NADPH | 80.3 | 0.1011 | 800 | pH 7, 25 °C | Open record: myDHFR (dihydrofolate reductase), NADPH |
| ppDHFR (dihydrofolate reductase) | Photobacterium profundum | dihydrofolate | 156 | 0.0062 | 2.52 × 10⁴ | pH 7, 25 °C | Open record: ppDHFR (dihydrofolate reductase), dihydrofolate |
| ppDHFR (dihydrofolate reductase) | Photobacterium profundum | NADPH | 150.5 | 0.0198 | 7.6 × 10³ | pH 7, 25 °C | Open record: ppDHFR (dihydrofolate reductase), NADPH |
| svDHFR (dihydrofolate reductase) | Shewanella violacea | dihydrofolate | 90.1 | 0.0019 | 4.74 × 10⁴ | pH 7, 25 °C | Open record: svDHFR (dihydrofolate reductase), dihydrofolate |
| svDHFR (dihydrofolate reductase) | Shewanella violacea | NADPH | 86.8 | 0.0034 | 2.55 × 10⁴ | pH 7, 25 °C | Open record: svDHFR (dihydrofolate reductase), NADPH |