CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic and structural analysis for potent antifolate inhibition of Pneumocystis jirovecii, Pneumocystis carinii, and human dihydrofolate reductases and their active-site variants

PMID 23545530doi:10.1128/aac.00172-13Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 51 records (101 values) from this paper: human dihydrofolate reductase (hDHFR), Pneumocystis carinii dihydrofolate reductase (pcDHFR), Pneumocystis jirovecii dihydrofolate reductase (pjDHFR) from Homo sapiens, Pneumocystis carinii, Pneumocystis jirovecii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
human dihydrofolate reductase (hDHFR)Homo sapiensdihydrofolic acid267 × 10⁻⁴3.7 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)Homo sapiensNADPH—0.0045—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant N64FHomo sapiensdihydrofolic acid288 × 10⁻⁴3.6 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant N64FHomo sapiensNADPH—0.0075—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant N64SHomo sapiensdihydrofolic acid369 × 10⁻⁴4.1 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant N64SHomo sapiensNADPH—0.0063—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensdihydrofolic acid395 × 10⁻⁴7.4 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensNADPH—0.0125—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensdihydrofolic acid434 × 10⁻⁴1.06 × 10⁵pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensNADPH—0.0057—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35K/N64SHomo sapiensdihydrofolic acid367 × 10⁻⁴5.1 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35K/N64SHomo sapiensNADPH—0.0066—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensdihydrofolic acid247 × 10⁻⁴3.7 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensNADPH—0.0038—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35S/N64FHomo sapiensdihydrofolic acid217 × 10⁻⁴3.2 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35S/N64FHomo sapiensNADPH—0.0056—pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensdihydrofolic acid234 × 10⁻⁴5.4 × 10⁴pH 7.4, 37 °COpen
human dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensNADPH—0.0053—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)Pneumocystis cariniidihydrofolic acid2350.00356.7 × 10⁴pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)Pneumocystis cariniiNADPH—0.023—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69NPneumocystis cariniiNADPH—0.0163—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69SPneumocystis cariniidihydrofolic acid2040.00712.9 × 10⁴pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant F69SPneumocystis cariniiNADPH—0.0192—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniidihydrofolic acid380.00666 × 10³pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniiNADPH—0.0072—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69NPneumocystis cariniidihydrofolic acid2230.01072.1 × 10⁴pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69NPneumocystis cariniiNADPH—0.0163—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69SPneumocystis cariniidihydrofolic acid1580.01859 × 10³pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37Q/F69SPneumocystis cariniiNADPH—0.0106—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37SPneumocystis cariniidihydrofolic acid450.01014 × 10³pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37SPneumocystis cariniiNADPH—0.0066—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69NPneumocystis cariniidihydrofolic acid1900.00972 × 10⁴pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69NPneumocystis cariniiNADPH—0.0167—pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69SPneumocystis cariniidihydrofolic acid1570.01161.4 × 10⁴pH 7.4, 37 °COpen
Pneumocystis carinii dihydrofolate reductase (pcDHFR)variant K37S/F69SPneumocystis cariniiNADPH—0.007—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciidihydrofolic acid360.0021.8 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciiNADPH—0.0189—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69FPneumocystis jiroveciidihydrofolic acid570.00321.8 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69FPneumocystis jiroveciiNADPH—0.0149—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69NPneumocystis jiroveciidihydrofolic acid780.00243.3 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37K/S69NPneumocystis jiroveciiNADPH—0.0864—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37QPneumocystis jiroveciidihydrofolic acid510.00351.5 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37QPneumocystis jiroveciiNADPH—0.0118—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69FPneumocystis jiroveciidihydrofolic acid260.0039 × 10³pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69FPneumocystis jiroveciiNADPH—0.0275—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69NPneumocystis jiroveciidihydrofolic acid950.00382.5 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S37Q/S69NPneumocystis jiroveciiNADPH—0.0173—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69FPneumocystis jiroveciidihydrofolic acid830.00481.7 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69FPneumocystis jiroveciiNADPH—0.0162—pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69NPneumocystis jiroveciidihydrofolic acid470.00341.4 × 10⁴pH 7.4, 37 °COpen
Pneumocystis jirovecii dihydrofolate reductase (pjDHFR)variant S69NPneumocystis jiroveciiNADPH—0.0252—pH 7.4, 37 °COpen