CatLogenzyme kinetics catalog

Values CatLog read from this paper

Atypical transient state kinetics of recombinant human dihydrofolate reductase produced by hysteretic behavior. Comparison with dihydrofolate reductases from other sources

PMID 2492521doi:10.1016/s0021-9258(19)81659-9Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 9 records (9 values) from this paper: dihydrofolate reductase from Homo sapiens, Escherichia coli, Gallus gallus and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductaseEscherichia coli7,8-dihydrofolate4.5——pH 7.65, 20 °COpen
dihydrofolate reductaseGallus gallus7,8-dihydrofolate2.6——pH 7.65, 20 °COpen
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 µM dihydrofolate and 100 µM NADPH; Table I7.9——pH 7.65, 20 °COpen
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; Table II11.6——pH 7.65, 20 °COpen
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat9.1——pH 7.65, 20 °COpen
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat11.7——pH 7.65, 20 °COpen
dihydrofolate reductaseHomo sapiens7,8-dihydrofolate100 mM 2-mercaptoethanol; 1.6 µM rHDHFR; apparent kcat8——pH 7.65, 20 °COpen
dihydrofolate reductasevariant F31LHomo sapiens7,8-dihydrofolate15.1——pH 7.65, 20 °COpen
dihydrofolate reductaseMus musculus7,8-dihydrofolate5.7——pH 7.65, 20 °COpen