CatLogenzyme kinetics catalog

Values CatLog read from this paper

Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers

PMID 7890613doi:10.1074/jbc.270.10.5057Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (19 values) from this paper: dihydrofolate reductase (hDHFR) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I23.8——pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I4.8——pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I—0.00386—pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I—3.3 × 10⁻⁴—pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolateTable I4.1——pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiens7,8-dihydrofolatein the text——1.5 × 10⁴pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22FHomo sapiensNADPH—0.00234—pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22RHomo sapiens7,8-dihydrofolateTable I0.0450.001628 calc.pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22RHomo sapiens7,8-dihydrofolatein the text——30pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22RHomo sapiensNADPH—0.0012—pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22WHomo sapiens7,8-dihydrofolateTable I4.194.2 × 10⁻⁴1.0 × 10⁴ calc.pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22WHomo sapiens7,8-dihydrofolatein the text——1 × 10⁴pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22WHomo sapiensNADPH—0.00135—pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22YHomo sapiens7,8-dihydrofolateTable I6.125.3 × 10⁻⁴1.2 × 10⁴ calc.pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22YHomo sapiens7,8-dihydrofolatein the text——1.2 × 10⁴pH 7.65, 20 °COpen
dihydrofolate reductase (hDHFR)variant L22YHomo sapiensNADPH—9.1 × 10⁻⁴—pH 7.65, 20 °COpen