Values CatLog read from this paper
The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major
PMID 9153248doi:10.1074/jbc.272.21.13883Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 29 records (29 values) from this paper: pteridine reductase 1 (PTR1), dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Leishmania major, Leishmania tarentolae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 1.5.1.3, EC 1.5.1.33, EC 2.1.1.45
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase-thymidylate synthase (DHFR-TS) | Leishmania major | folate | — | 0.0041 | — | pH 5 | Open record: dihydrofolate reductase-thymidylate synthase (DHFR-TS), folate |
| pteridine reductase 1 (PTR1) | Leishmania major | Biopterinrecombinant; expressed in E. coli (pET-3a) | — | 0.0122 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), Biopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | Biopterin | — | 0.0198 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), Biopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | Biopterin | — | 0.0399 | — | pH 7, 30 °C | Open record: pteridine reductase 1 (PTR1), Biopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | Biopterinnative; purified from L. major ptr1-/+PTR1 promastigotes | — | 0.0101 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), Biopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrobiopterin | — | 0.0076 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrobiopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrobiopterin | — | 0.0056 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrobiopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrobiopterin | — | 0.0054 | — | pH 7, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrobiopterin |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrofolate | — | 0.0061 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrofolate |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrofolate | — | 0.0034 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrofolate |
| pteridine reductase 1 (PTR1) | Leishmania major | dihydrofolate | — | 0.0054 | — | pH 7, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrofolate |
| pteridine reductase 1 (PTR1) | Leishmania major | Folate | — | 0.0016 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), Folate |
| pteridine reductase 1 (PTR1) | Leishmania major | Folaterecombinant; expressed in E. coli (pET-3a) | — | 0.0026 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), Folate |
| pteridine reductase 1 (PTR1) | Leishmania major | Folate | — | 0.0085 | — | pH 7, 30 °C | Open record: pteridine reductase 1 (PTR1), Folate |
| pteridine reductase 1 (PTR1) | Leishmania major | Folatenative; purified from L. major ptr1-/+PTR1 promastigotes | — | 0.0024 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), Folate |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHBiopterin; cosubstrate Biopterin; recombinant | — | 0.0132 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHH_2biopterin; cosubstrate H_2biopterin; recombinant | — | 0.0145 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHFolate fixed (Table I footnote 1-a); cosubstrate Folate | — | 0.0122 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHH_2folate fixed (Table I footnote 1-a); recombinant | — | 0.0142 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHBiopterin; cosubstrate Biopterin; native | — | 0.0116 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania major | NADPHFolate fixed (Table I footnote 1-a); cosubstrate Folate | — | 0.0135 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | Biopterin | — | 0.0109 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), Biopterin |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | dihydrobiopterin | — | 0.0085 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrobiopterin |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | dihydrofolate | — | 0.0067 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), dihydrofolate |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | Folate | — | 0.0019 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), Folate |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | NADPHBiopterin; cosubstrate Biopterin | — | 0.0123 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | NADPHH_2biopterin; cosubstrate H_2biopterin | — | 0.00935 | — | pH 4.7, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | NADPHFolate fixed (Table I footnote 1-a); cosubstrate Folate | — | 0.0146 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |
| pteridine reductase 1 (PTR1) | Leishmania tarentolae | NADPHH_2folate fixed (Table I footnote 1-a) | — | 0.012 | — | pH 6, 30 °C | Open record: pteridine reductase 1 (PTR1), NADPH |