Values CatLog read from this paper
"Catch 222," the effects of symmetry on ligand binding and catalysis in R67 dihydrofolate reductase as determined by mutations at Tyr-69
PMID 15333637doi:10.1074/jbc.m404485200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: R67 dihydrofolate reductase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| R67 dihydrofolate reductasevariant Y69F:1 | — | dihydrofolate | 0.98 | 0.021 | 47 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.0064 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69F:1+2 | — | dihydrofolate | 0.96 | 0.02 | 48 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1+2 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.014 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69F:1+2+3 | — | dihydrofolate | 1.4 | 0.035 | 40 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2+3, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1+2+3 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.021 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2+3, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69F:1+2+3+4 | — | dihydrofolate | 1.4 | 0.054 | 26 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2+3+4, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1+2+3+4 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.04 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+2+3+4, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69F:1+3 | — | dihydrofolate | 0.56 | 0.02 | 28 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+3, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1+3 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.0031 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+3, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69F:1+4 | — | dihydrofolate | 1.9 | 0.028 | 68 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+4, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69F:1+4 | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.014 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69F:1+4, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductasevariant Y69L | — | dihydrofolate | 0.16 | 0.18 | 0.89 calc. | pH 7 | Open record: R67 dihydrofolate reductase variant Y69L, dihydrofolate |
| R67 dihydrofolate reductasevariant Y69L | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.068 | — | pH 7 | Open record: R67 dihydrofolate reductase variant Y69L, reduced nicotinamide adenine dinucleotide phosphate |