Values CatLog read from this paper
Role of S65, Q67, I68, and Y69 residues in homotetrameric R67 dihydrofolate reductase
PMID 11560482doi:10.1021/bi0110544Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (18 values) from this paper: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68L | — | dihydrofolate | 0.32 | 0.024 | 13 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant I68L, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68L | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.026 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant I68L, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68M | — | dihydrofolate | 0.17 | 0.025 | 6.8 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant I68M, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant I68M | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.021 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant I68M, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Q67C | — | dihydrofolate | 0.1 | 0.055 | 1.8 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Q67C, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Q67C | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.026 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Q67C, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant S65A | — | dihydrofolate | 1.1 | 0.004 | 280 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant S65A, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant S65A | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.0029 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant S65A, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69F | — | dihydrofolate | 2.5 | 0.044 | 57 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Y69F, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69F | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.066 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Y69F, reduced nicotinamide adenine dinucleotide phosphate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69H | — | dihydrofolate | 0.014 | 0.046 | 0.30 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Y69H, dihydrofolate |
| R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR)variant Y69H | — | reduced nicotinamide adenine dinucleotide phosphate | — | 0.176 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase (type II R-plasmid-encoded DHFR) variant Y69H, reduced nicotinamide adenine dinucleotide phosphate |