Values CatLog read from this paper

Probing the roles of conserved arginine-44 of Escherichia coli dihydrofolate reductase in its function and stability by systematic sequence perturbation analysis

PMID 20043879doi:10.1016/j.bbrc.2009.12.134Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (60 values) from this paper: dihydrofolate reductase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dihydrofolate reductase	Escherichia coli	dihydrofolate	5.4	0.0016	3.4 × 10³	15 °C	Open record: dihydrofolate reductase, dihydrofolate
dihydrofolate reductasevariant R44A	Escherichia coli	dihydrofolate	3.05	0.0016	1.9 × 10³	15 °C	Open record: dihydrofolate reductase variant R44A, dihydrofolate
dihydrofolate reductasevariant R44C	Escherichia coli	dihydrofolate	2.43	0.0011	2.2 × 10³	15 °C	Open record: dihydrofolate reductase variant R44C, dihydrofolate
dihydrofolate reductasevariant R44D	Escherichia coli	dihydrofolate	0.69	0.003	230	15 °C	Open record: dihydrofolate reductase variant R44D, dihydrofolate
dihydrofolate reductasevariant R44E	Escherichia coli	dihydrofolate	0.89	0.0019	470	15 °C	Open record: dihydrofolate reductase variant R44E, dihydrofolate
dihydrofolate reductasevariant R44F	Escherichia coli	dihydrofolate	1.39	0.0027	500	15 °C	Open record: dihydrofolate reductase variant R44F, dihydrofolate
dihydrofolate reductasevariant R44G	Escherichia coli	dihydrofolate	1.78	0.0015	1.2 × 10³	15 °C	Open record: dihydrofolate reductase variant R44G, dihydrofolate
dihydrofolate reductasevariant R44H	Escherichia coli	dihydrofolate	1.59	7 × 10⁻⁴	2.5 × 10³	15 °C	Open record: dihydrofolate reductase variant R44H, dihydrofolate
dihydrofolate reductasevariant R44I	Escherichia coli	dihydrofolate	1.15	0.0021	560	15 °C	Open record: dihydrofolate reductase variant R44I, dihydrofolate
dihydrofolate reductasevariant R44K	Escherichia coli	dihydrofolate	5.7	0.0025	2.3 × 10³	15 °C	Open record: dihydrofolate reductase variant R44K, dihydrofolate
dihydrofolate reductasevariant R44L	Escherichia coli	dihydrofolate	0.98	0.0014	680	15 °C	Open record: dihydrofolate reductase variant R44L, dihydrofolate
dihydrofolate reductasevariant R44M	Escherichia coli	dihydrofolate	3.8	0.0023	1.6 × 10³	15 °C	Open record: dihydrofolate reductase variant R44M, dihydrofolate
dihydrofolate reductasevariant R44N	Escherichia coli	dihydrofolate	3.3	0.0025	1.3 × 10³	15 °C	Open record: dihydrofolate reductase variant R44N, dihydrofolate
dihydrofolate reductasevariant R44P	Escherichia coli	dihydrofolate	0.3	0.0029	100	15 °C	Open record: dihydrofolate reductase variant R44P, dihydrofolate
dihydrofolate reductasevariant R44Q	Escherichia coli	dihydrofolate	1.9	0.0024	800	15 °C	Open record: dihydrofolate reductase variant R44Q, dihydrofolate
dihydrofolate reductasevariant R44S	Escherichia coli	dihydrofolate	5.8	0.0031	1.9 × 10³	15 °C	Open record: dihydrofolate reductase variant R44S, dihydrofolate
dihydrofolate reductasevariant R44T	Escherichia coli	dihydrofolate	2	0.0025	800	15 °C	Open record: dihydrofolate reductase variant R44T, dihydrofolate
dihydrofolate reductasevariant R44V	Escherichia coli	dihydrofolate	1.31	8 × 10⁻⁴	1.7 × 10³	15 °C	Open record: dihydrofolate reductase variant R44V, dihydrofolate
dihydrofolate reductasevariant R44W	Escherichia coli	dihydrofolate	2.41	0.0037	660	15 °C	Open record: dihydrofolate reductase variant R44W, dihydrofolate
dihydrofolate reductasevariant R44Y	Escherichia coli	dihydrofolate	0.89	0.001	900	15 °C	Open record: dihydrofolate reductase variant R44Y, dihydrofolate